A5058760209- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Protein Interaction Studies and Fluorescence Analysis
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Antioxidant Activity and Oxidative Stress
- Organ Transplantation Techniques and Outcomes
- Diet and metabolism studies
- Anesthesia and Sedative Agents
- Drug-Induced Hepatotoxicity and Protection
- Dialysis and Renal Disease Management
- Neonatal Health and Biochemistry
- Electron Spin Resonance Studies
- Hemoglobin structure and function
- Peroxisome Proliferator-Activated Receptors
- Vitamin C and Antioxidants Research
- Radical Photochemical Reactions
- Advanced Glycation End Products research
- Stress Responses and Cortisol
- Epilepsy research and treatment
- Blood Pressure and Hypertension Studies
- Drug Transport and Resistance Mechanisms
- Porphyrin Metabolism and Disorders
- Cholesterol and Lipid Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
Medical University of Graz
2013-2023
University College London
2021
Canon (Japan)
2016
European Foundation for the Study of Chronic Liver Failure
2016
University of Graz
1997-2011
National Security Agency
2007
Acute-on-chronic liver failure (ACLF) in cirrhosis is characterized by acute decompensation (AD), organ failure(s), and high short-term mortality. Recently, we have proposed (systemic inflammation [SI] hypothesis) that ACLF the expression of an exacerbation SI already present decompensated cirrhosis. This study was aimed at testing this hypothesis included 522 patients with (237 ACLF) 40 healthy subjects. assessed measuring 29 cytokines redox state circulating albumin (HNA2), a marker...
Background: Patients with acutely decompensated cirrhosis (AD) may or not develop acute-on-chronic liver failure (ACLF). ACLF is characterized by high-grade systemic inflammation, organ failures (OF) and high short-term mortality. Although patients AD exhibit distinct clinical phenotypes at baseline, they have low mortality, unless develops during follow-up. Because little known about the association of profile inflammation cirrhosis, we aimed to investigate a battery markers in these...
•In a first-in-man, randomized-controlled trial of DIALIVE vs. standard care, the primary endpoint safety was met.•DIALIVE achieved acceptable performance characteristics for albumin exchange and reduction in endotoxin.•DIALIVE significantly reduced time to resolution ACLF improved prognostic scores compared with care.•DIALIVE had greater impact on pathophysiologically relevant biomarkers associated ACLF. Background & AimsAcute-on-chronic liver failure (ACLF) is characterized by severe...
alpha-Tocopherol (alpha-T), an important anti-oxidant of plasma lipoproteins and cell membranes, is secreted from liver together with very-low-density into the blood stream. Other serum lipoprotein classes gain alpha-T by exchange transfer processes. We show here that lipoprotein-free d > 1.22 g/ml fraction human or pig increases rate a factor 2-4 as compared spontaneous exchange/transfer. The exchange/transfer (alpha-TET) activity was purified multiple-step column chromatography. It...
Albumin is the most abundant plasma protein. Critical illness often associated with altered, predominately decreased, serum albumin levels. This hypoalbuminaemia usually corrected by administration of exogenous albumin. study aimed to track concentration-dependent influence on blood coagulation in vitro. Whole (WB) samples from 25 volunteers were prepared contain low (19.3 ± 7.7 g/L), physiological (45.2 7.8 and high (67.5 18.1 g/L) levels Haemostatic profiling was performed using a platelet...
The question arises as to whether oxidative stress has a primary role in neurodegeneration or is secondary end-stage epiphenomenon. aim of the present study was determine parameters like malondialdehyde (MDA), carbonyl proteins (CP) and Albumin-disulphide (Alb-SSR) relate these immune parameter neopterin, folic acid vitamin B12 vitamins homocysteine patients with neuro-degenerative diseases (NDD), namely mild cognitive impairment (MCI) Alzheimer's disease (AD) compared an aged matched...
Systemic inflammation (SI) is involved in the pathogenesis of acute decompensation (AD) and acute‐on‐chronic liver failure (ACLF) cirrhosis. In other diseases, SI activates tryptophan (Trp) degradation through kynurenine pathway (KP), giving rise to metabolites that contribute multiorgan/system damage immunosuppression. current study, we aimed characterize KP patients with cirrhosis, whom this poorly known. The serum levels Trp, key (kynurenine kynurenic quinolinic acids), cytokines (SI...
Decompensated cirrhosis is characterized by exuberant systemic inflammation. Although the inducers of this feature remain unknown, presence circulating forms oxidized albumin, namely human nonmercaptalbumin 1 (HNA1) and HNA2, a common finding in cirrhosis. The aim study was to explore ability these albumin induce inflammation triggering activation peripheral leukocytes. We observed significantly higher plasma levels HNA1 HNA2 patients with (n = 256) compared healthy volunteers 48), which...
Advanced oxidation protein products (AOPPs) are carried by oxidized plasma proteins, especially albumin and accumulate in subjects with renal disease coronary artery disease. AOPPs represent an excellent novel marker of oxidative stress their roles the development cardiovascular might be great importance. Here, we show that vitro-generated AOPP-albumin binds high affinity to high-density lipoprotein (HDL) receptor scavenger class B type I (SR-BI). Already equimolar concentration HDL blocked...
The purpose of this study was to investigate the effect single bouts exercise at three different intensities on redox state human serum albumin (HSA) and carbonyl groups protein (CP) concentrations in plasma. Trained men [ n = 44, maximal oxygen consumption (V̇o 2max ): 55 ± 5 ml·kg −1 ·min , nonsmokers, 34 years age] from a homogenous population, volunteers police special forces unit, were randomly assigned perform cycle ergometer either 70% ( 14), 75% or 80% 16) V̇o for 40 min. Blood...
Abstract Albumin is the major plasma protein with several important biological functions. Various disturbances of albumin function have been demonstrated in end‐stage liver disease. These functional may be related to oxidative modifications at cysteine‐34, including irreversibly oxidized human nonmercaptalbumin‐2 ( HNA2 ). The aim present study was relate modification short‐term prognosis chronic failure. Patients advanced cirrhosis N = 39), acute‐on‐chronic failure 15), and healthy controls...
Abstract Human serum albumin (HSA) constitutes the primary transporter of fatty acids, bilirubin, and other plasma compounds. The binding, transport, release its cargos strongly depend on conformation, which is affected by bound ligands induced physiological pathological conditions. HSA both highly oxidized heavily loaded with acids bilirubin in chronic liver disease. By employing small-angle X-ray scattering we show that from disease patients undergoes a distinct opening compared to healthy...
Oxidative stress is believed to play an important role in acute-on-chronic liver failure (AoCLF). Albumin, transport vehicle, was found be severely oxidized AoCLF patients. Extracorporeal support systems may exert beneficial effects via removal of albumin-bound toxins. At present, two are commercially available, the molecular adsorbents recirculating system (MARS) and fractionated plasma separation, adsorption dialysis (FPAD, also known as Prometheus). The aim this study compare effect MARS...
To assess the effects of different exercise intensities and antioxidant supplementation on plasma protein modification.Trained men (n = 41) from a homogenous population were randomly assigned to perform cycle ergometer either at 70% or 80% individual .VO2max. Each intensity group was receive juice powder concentrate (JPC 70%, n 11; JPC 80%, 10) placebo (Plac 10; Plac capsules for 28 wk. Four controlled bouts blood collections conducted baseline study weeks 4, 16, 28. Blood samples drawn...
We have previously shown that the modification of high-density lipoprotein subclass 3 (HDL(3)) by HOCl transformed an anti-atherogenic into a high-uptake form for macrophages and caused significant impairment cholesterol efflux capacity [Panzenboeck, Raitmayer, Reicher, Lindner, Glatter, Malle Sattler (1997) J. Biol. Chem. 272, 29711-29720]. To elucidate consequences treatment with OCl(-) on distinct regions in apolipoprotein A-I (apo A-I), lipid-free lipid-associated apo were modified...