A5017814895- Nanoparticle-Based Drug Delivery
- Chemical Synthesis and Analysis
- Extracellular vesicles in disease
- Characterization and Applications of Magnetic Nanoparticles
- Advanced biosensing and bioanalysis techniques
- Alzheimer's disease research and treatments
- Geomagnetism and Paleomagnetism Studies
- Nanoplatforms for cancer theranostics
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Autophagy in Disease and Therapy
- Advanced Nanomaterials in Catalysis
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Bone Tissue Engineering Materials
- Iron Metabolism and Disorders
- Supramolecular Self-Assembly in Materials
- Peptidase Inhibition and Analysis
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Hippo pathway signaling and YAP/TAZ
- Chronic Kidney Disease and Diabetes
- Biomarkers in Disease Mechanisms
National Center for Nanoscience and Technology
2016-2025
Chinese Academy of Sciences
2016-2023
First Affiliated Hospital of Jinan University
2023
University of Chinese Academy of Sciences
2019-2023
Jinan University
2022
Peking University
2012-2013
Abstract Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs the potential blood contact. However, so far, most studies are not systematic and provided insights into mechanisms of AgNPs. In this study, we biological effects, including hemolysis, lymphocyte proliferation, platelet aggregation, coagulation complement activation, 20 nm with two different surface coatings...
Hypoxia is one of the most important factors that limit effect radiotherapy, and abundant H2O2 in tumor tissues will also aggravate hypoxia-induced radiotherapy resistance. Delivering catalase to decompose into oxygen an effective strategy relieve hypoxia However, low stability limits catalase's vivo application, which common limitations for almost all proteins' internal utilization. Here, we develop containing E. coli membrane vesicles (EMs) with excellent protease resistance a long time....
PTEN-putative kinase 1 (PINK1)-Parkin-mediated mitophagy is crucial for the clearance of damaged mitochondria. However, mechanisms underlying PINK1-Parkin-mediated are not fully understood. The goal this study to identify new regulators and elucidate regulatory mitophagy.Quantitative mitochondrial proteomic analysis revealed that 63 proteins showed increased levels 36 decreased in cells subjected carbonyl cyanide m-chlorophenyl hydrazone (CCCP) treatment. Peroxiredoxin 6 (PRDX6 or Prx6), a...
Abstract Rheumatoid arthritis (RA) seriously impairs the quality of life sufferers. It has been shown that Lycium barbarum polysaccharide (LBP), a natural active indigestible ingredient with medicinal and edible functions, can effectively relieve RA, however, whether this effect is related to gut microbiota not known. This study aimed explore RA alleviating mechanism LBP mediated by using collagen-induced rat model. The results showed significantly changed microflora structure accompanied...
The targeted elevation of the labile iron pool (LIP) represents most direct and effective strategy to induce ferroptosis in cancer cells. However, efficiency increasing LIP via supplementation is controversial due homeostasis between storage pool, leading poor effects serious safety concerns. In this study, a bio-nanocomplex named AbDA-Lim, composed albumin, polydopamine, limonene, prepared promote non-canonical cells by destroying balance. Albumin avidity drives AbDA-Lim entering...
A universal strategy for efficient, mild, and purification-free synthesis in situ screening of functional polymer-peptide nanomaterials is described. More than 1000 polymer–peptide conjugates (PPCs) with various chemical structures, compositions, therapeutic efficacy are created. According to this strategy, the structure–function relationship PPCs revealed, antitumor efficacies top performing evaluated vivo. As a service our authors readers, journal provides supporting information supplied...
Abstract Defective rhodopsin homeostasis is one of the major causes retinal degeneration, including disease Retinitis pigmentosa. To identify cellular factors required for biosynthesis rhodopsin, we performed a genome-wide genetic screen in Drosophila mutants with reduced levels rhodopsin. We isolated loss-of-function alleles endoplasmic reticulum membrane protein complex 3 ( emc3 ), emc5 , and emc6 each which exhibited defective phototransduction photoreceptor cell degeneration. EMC3, EMC5,...
Exposure to atmospheric particulate matter (PM) is a frequent occurrence humans, and their adverse outcomes have become global concern. Although PM-induced inflammation common phenomenon, clear picture of the mechanisms underlying exosome-mediated PM has not yet emerged. Here, we show that exosomes can mediate cascade reactions for transfer inflammatory responses macrophages. Specifically, two fine PM2.5, namely F1 (<0.49 μm) F2 (0.95–1.5 μm), stimulated substantial release from macrophages...
To identify peptides with high affinity and specificity against human epidermal growth factor receptor 2 (HER2), a series of were designed based on the structure HER2 its Z(HER2:342) affibody.By using combination protocol molecular dynamics modeling, MM/GBSA binding free energy calculations, decomposition analysis, two novel 27 residues, pep27 pep27-24M, successfully obtained.Immunocytochemistry flow cytometry analysis verified that both can specifically bind to extracellular domain protein...
Breast cancer is the most common malignant disease in women worldwide. Early diagnosis and treatment can greatly improve management of breast cancer. Liquid biopsies are becoming convenient detection methods for diagnosing monitoring due to their noninvasiveness ability provide real-time feedback. A range liquid biopsy markers, including circulating tumor proteins, cells, nucleic acids, have been implemented prognosis, with each having its own advantages limitations. Circulating...
Abstract pH‐dependent peptide biomaterials hold tremendous potential for cell delivery and tissue engineering. However, identification of responsive self‐assembling sequences with specified secondary structure remains a challenge. In this work, An experimental procedure based on the one‐bead one‐compound (OBOC) combinatorial library is developed to rapidly screen β‐sheet peptides at neutral aqueous solution (pH 7.5) disassemble weak acidic condition 6.5). Using hydrophobic fluorescent...
Derived from magnetotactic bacteria (MTB), magnetosomes consist of magnetite crystals enclosed within a lipid bilayer membrane and are known to possess advantages over artificially synthesized nanoparticles because the narrow size distribution, uniform morphology, high purity crystallinity, single magnetic domain, good biocompatibility, easy surface modification. These unique properties have increasingly attracted researchers apply bacterial (BMs) in fields biology medicine as MRI imaging...
Amyloid-beta (Aβ) aggregation plays an important role in the development of Alzheimer's disease (AD). In AD brain, amyloid plaques are surrounded by reactive astrocytes, and many essential functions astrocytes have been reported to be mediated protein secretion. However, roles activated progression under intense debate. To provide in-depth view secretomes we present this study a quantitative profile rat hippocampal astrocyte at multiple time points after both brief sustained Aβ(1-42)...
Nanoscaled polymer-peptide conjugates (PPCs) containing both functional peptides and synthetic polymer comprise a new family of biomaterials that can circumvent the limitation alone. Our previous work showed PPCs with therapeutic peptide KLAK, especially shorter PEG spacers higher degree polymerization, exhibit enhanced antitumor effects through disrupting mitochondrial membranes. However, as have spherical nanostructure (45-60 nm), this may other besides conjugated KLAK itself when they...
Although the toxicity and molecular mechanisms of graphene oxide (GO) have been reported for several cell types, no proteomic study GO has yet conducted on macrophage cells. In this study, we used proteomics based stable isotope labeling with amino acids in culture (SILAC) to quantify changes RAW 264.7 cells following treatment. We found 73 proteins that were significantly dysregulated after The down-regulated included many ribosomal subunit proteins, indicating affected growth. most...
It is well known that when exposed to human blood plasma, nanoparticles are predominantly coated by a layer of proteins, forming corona will mediate the subsequent cell interactions. Magnetosomes protein-rich membrane which synthesized magnetic bacteria; these have gained lot attention owing their unique and biochemical characteristics. Nevertheless, whether bacterial magnetosomes after interacting with how such affects nanoparticle-cell interactions yet be elucidated. The aim this study was...
The One-Bead One-Compound (OBOC) library screening is an efficient technique for identifying targeting peptides. However, due to the relatively large bead size, it challenging OBOC method be applied in vivo screening. Herein, we report Localized Instillation Beads (LIB) discover peptides with technique. Inspired by localized instillation, constructed a cavity inside of transplanted tumor mouse. Then, heptapeptide was injected and incubated cavity. After elution process, retained beads were...