A5015704722- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- Neurogenesis and neuroplasticity mechanisms
- Amyotrophic Lateral Sclerosis Research
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Neurogenetic and Muscular Disorders Research
- Autophagy in Disease and Therapy
- Retinal Development and Disorders
- Neurological disorders and treatments
- Cholinesterase and Neurodegenerative Diseases
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- S100 Proteins and Annexins
- Genetics and Plant Breeding
- Tea Polyphenols and Effects
- Morinda citrifolia extract uses
- RNA Interference and Gene Delivery
The University of Texas Southwestern Medical Center
2020-2024
South China Agricultural University
2020-2024
Center for Neuroscience and Regenerative Medicine
2024
Key Laboratory of Guangdong Province
2022
Peking University
2013-2020
Guangzhou Experimental Station
2017
Chinese Academy of Tropical Agricultural Sciences
2017
Aims: Parkinson's disease (PD) heavily affects humans and little is known about its cause pathogenesis. Sirtuin 3 (Sirt3) plays a key role in regulating mitochondrial dysfunction, which the main of DAergic neuronal loss PD. We investigated mechanisms neuroprotective Sirt3 survival. Results: was reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP)-treated neurons with overexpression being neuroprotective. identified that interacted manganese superoxide dismutase (SOD2) adenosine...
Rice (Oryza sativa L.) is a major dietary source of cadmium (Cd). Developing rice varieties with reduced Cd levels in the grain cost-effective and practical approach to enhance food safety, particularly regions high contamination. However, genetic mechanisms underlying accumulation grains are not fully understood. In this study, we identified eight quantitative trait loci (QTLs) associated through substitution mapping using single segment lines (SSSLs). These QTLs, named qCd‐2‐1, qCd‐3‐1,...
PTEN-putative kinase 1 (PINK1)-Parkin-mediated mitophagy is crucial for the clearance of damaged mitochondria. However, mechanisms underlying PINK1-Parkin-mediated are not fully understood. The goal this study to identify new regulators and elucidate regulatory mitophagy.Quantitative mitochondrial proteomic analysis revealed that 63 proteins showed increased levels 36 decreased in cells subjected carbonyl cyanide m-chlorophenyl hydrazone (CCCP) treatment. Peroxiredoxin 6 (PRDX6 or Prx6), a...
DYT1 dystonia is a hereditary neurologic movement disorder characterized by uncontrollable muscle contractions. It caused heterozygous mutation in Torsin A ( TOR1A ), gene encoding membrane-embedded ATPase. While animal models provide insights into disease mechanisms, significant species-dependent differences exist since animals with the identical fail to show pathology. Here, we model using human patient-specific cholinergic motor neurons (MNs) that are generated through either direct...
Amyloid plaques are crucial for the pathogenesis of Alzheimer disease (AD). Phagocytosis fibrillar β-amyloid (Aβ) by activated microglia is essential Aβ clearance in disease. However, mechanism underlying remains unclear. In this study, we performed stable isotope labeling amino acids cultured cells quantitative proteomics analysis to determine changes protein expression BV2 treated with or without Aβ. Among 2742 proteins identified, six were significantly up-regulated and seven...
Abstract Effective therapeutics is much needed for amyotrophic lateral sclerosis (ALS), an adult-onset neurodegenerative disease mainly affecting motor neurons. By screening chemical compounds in human patient-derived and aging-relevant neurons, we identify a neuroprotective compound show that MAP4Ks may serve as therapeutic targets treating ALS. The lead broadly improves survival function of neurons directly converted from ALS patients. Mechanistically, it works inhibitor MAP4Ks, regulates...
Abstract HOTAIR (homeobox transcript antisense RNA), one of the prototypical long non-coding RNAs, has been verified overexpressed in multiple carcinomas and emerged as a promising novel anticancer target. Its well-established role is acting predictor poor prognosis promoting cancer cell metastasis. Recently, another important mission was uncovered that targeting caused apoptosis. Nevertheless, so far there no published data elaborating mechanism. Here, we report microRNA miR-125a-5p...
Grain size is a key factor influencing grain yield and appearance quality in rice. We identified twelve quantitative trait loci (QTL) for length (GL), nine width (GW), 1000-kernel weight (TKW) using GLU-SSSLs, which are single-segment substitution lines with Oryza glumaepatula as donor parent Huajingxian 74 (HJX74) recipient parent. Among the QTL, qGL1-2, qGL1-4, qGL9-2, qGW2-2, qGW9-1 qTKW9-2 contributed to high yield. GL9 was candidate gene qGL9-2 by map-based cloning sequencing, novel...
Abstract Background Alzheimer’s disease (AD) is an adult-onset mental disorder with aging as a major risk factor. Early and progressive degeneration of basal forebrain cholinergic neurons (BFCNs) contributes substantially to cognitive impairments AD. An aging-relevant cell model BFCNs will critically help understand AD identify potential therapeutics. Recent studies demonstrate that induced directly reprogrammed from adult human skin fibroblasts retain aging-associated features. However,...
ABSTRACT In vivo reprogramming of glia into functional neurons emerges as potential regeneration-based therapeutics for neural injuries or neurological diseases. Recent studies show that AAV-based manipulation certain factors can rapidly and highly efficiently convert resident with brain region-specificity precise connectivity. Using NEUROD1 an example, we here the presumed astrocytes-converted are essentially endogenous in adult mouse brain. AAV-mediated co-expression a reporter indeed...
Litchi (Litchi chinensis Sonn.) is an important fruit that widely cultivated in tropical and subtropical areas. In this study, we used RNA-Seq iTRAQ technologies to compare the transcriptomes proteomes of pollinated (polLFs) parthenocarpic (parLFs) litchi fruits during early development (1 day, 2 days, 4 days 6 days). We identified 4,864 DEGs polLFs 3,672 parLFs, which 2,835 were shared 1,051 specifically parLFs. Compared po1LFs, 768 analysis 551 DEPs 1,021 305 526 exclusively found 1,127...
Abstract Epistasis plays an important role in manipulating rice tiller number, but epistatic mechanism still remains a challenge. Here we showed the process of analysis between tillering QTLs. A half diallel mating scheme was conducted based on 6 single segment substitution lines and 9 dual pyramiding to allow 4 components. Additive-additive, additive-dominance, dominance-additive, dominance-dominance effects were estimated at stages development via unconditional QTL simultaneously....
Fas apoptosis inhibitory molecule (FAIM) is a highly conserved anti-apoptotic protein which plays important roles in cells. There are two isoforms of FAIM, the short isoform FAIM-S broadly expressed all tissues, whereas long FAIM-L exclusively nervous system. No structure human FAIM has been reported to date and detailed molecular mechanisms underlying function remain unknown. Here, crystal N-terminal domain (NTD) NMR solution C-terminal (CTD) were determined. The structures revealed that...
The mitogen-activated protein kinase kinases (MAP4Ks) signaling pathway plays a pivotal role in axonal regrowth and neuronal degeneration following insults. Whether targeting this is beneficial to brain injury remains unclear. In study, we showed that adeno-associated virus-delivery of the Citron homology domain MAP4Ks effectively reduces traumatic injury-induced reactive gliosis, tauopathy, lesion size, behavioral deficits. Pharmacological inhibition replicated ameliorative effects observed...
ABSTRACT Effective therapeutics is much needed for amyotrophic lateral sclerosis (ALS), an adult-onset neurodegenerative disease mainly affecting motor neurons. By screening chemical compounds in human patient-derived and aging-relevant neurons, we identify a neuroprotective compound show that MAP4Ks may serve as therapeutic targets treating ALS. The lead broadly improves survival function of neurons directly converted from ALS patients. Mechanistically, it works inhibitor MAP4Ks, regulates...
Abstract DYT1 dystonia is a hereditary neurological disease caused by heterozygous mutation in torsin A ( TOR1A ). While animal models provide insights into mechanisms, significant species-dependent differences exist since mice with the identical fail to show pathology. Here, we model using human patient-derived motor neurons. These neurons markedly thickened nuclear lamina, disrupted morphology, and impaired nucleocytoplasmic transport, whereas they lack perinuclear “blebs” that are often...
Abstract Grain size and grain number play extremely important roles in rice yield. Here, we identify GW10 , which encodes a P450 subfamily protein controlls by using Lemont ( tropical japonica ) as donor parent HJX74 indica recipient parent. The locus was mapped into 20.1 kb region on the long arm of Chromosome 10. Lower expression gw10 panicle is contributed to shorter narrower grain, increased grains per panicle. Furthermore, higher levels some brassinosteroid (BR) biosynthesis response...
In recent years, Millettia specisoa Champ has attracted extensive attention because of its high medicinal and edible value. This paper will summarize the composition application Champ, analyze advantages existing problems in developing industry Guangdong Province according to demand for present situation Province, put forward reasonable countermeasures suggestions.