A5026433435- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Prostate Cancer Treatment and Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Urinary Bladder and Prostate Research
- Prostate Cancer Diagnosis and Treatment
- Reconstructive Surgery and Microvascular Techniques
- Chronic Myeloid Leukemia Treatments
- Pelvic floor disorders treatments
- Vascular Procedures and Complications
- Peripheral Artery Disease Management
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Urologic and reproductive health conditions
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Telomeres, Telomerase, and Senescence
Dalian Medical University
2011-2024
Second Affiliated Hospital of Dalian Medical University
2011-2024
North Sichuan Medical University
2024
Affiliated Hospital of North Sichuan Medical College
2024
First Affiliated Hospital of Xi'an Jiaotong University
2015-2022
Xi'an Jiaotong University
2015-2020
University of Oslo
2004-2006
Norwegian Cancer Society
2005-2006
Oslo University Hospital
2004
A therapeutic strategy that targets multiple proinflammatory factors in inflammatory bowel disease (IBD) with minimal systemic side effects would be attractive. Here, we develop a drug-free, biodegradable nanomedicine acts against IBD by scavenging cell-free DNA (cfDNA) and reactive oxygen species (ROS). Polyethylenimine (PEI) was conjugated to antioxidative diselenide-bridged mesoporous organosilica nanoparticles (MONs) formulate (MON-PEI) exhibited high cfDNA binding affinity...
Inflammatory bowel disease (IBD) is characterized by chronic relapsing inflammation of the gastrointestinal tract. Oxidative stress plays a pivotal role in pathogenesis IBD. Selenium-containing amino acids reportedly have anti-oxidative and anti-inflammatory properties, but it remains unknown if selenium-containing can be used to treat This study aimed investigate effects two - selenocysteine selenocystine on oxidative mouse model dextran sulfate sodium (DSS)-induced IBD.C57BL/6 mice were...
Here, we present results from a clinical trial employing new vaccination method using dendritic cells (DCs) transfected with mRNA allogeneic prostate cancer cell lines (DU145, LNCaP and PC-3). In all, 20 patients were enrolled 19 have completed vaccination. Each patient received at least four weekly injections 2 × 107 DCs either intranodally or intradermally. Safety feasibility of determined. Immune responses measured as delayed-type hypersensitivity by in vitro immunoassays including...
MicroRNAs, a kind of small non-coding RNAs, can regulate gene expression by targeting mRNAs for translational repression or degradation. Much evidence has suggested that miR-218 was tumor suppressor in many human cancers including prostate cancer. However, the underlying role angiogenesis and mechanisms PCa other remains to be unclear. Here this present study, we demonstrated inhibited cells vitro vivo. RICTOR, mTOR component 2, direct target miR218-RICTOR-VEGFA axis mechanism inhibiting...
Abstract Tumor cells dying after cytotoxic therapy are a potential source of antigen for T-cell priming. Antigen-presenting (APC) can cross-present MHC I–restricted peptides the uptake cells. Depending on nature surrounding environmental signals, APCs then orchestrate spectrum responses ranging from immune activation to inhibition. Previously, we had demonstrated that combining radiation with either agonistic monoclonal antibody (mAb) CD40 or systemically administered TLR7 agonist could...
Abstract With the aim of producing large quantities mRNA‐transfected monocyte‐derived dendritic cells (DCs) to be used as cancer vaccines, a new clinical grade procedure has been developed. Peripheral blood mononuclear (PBMCs) obtained by leukapheresis were enriched for monocytes immunomagnetic depletion CD19 + B and CD2 T employing ISOLEX 300i device. After 5 days culture in gas permeable Teflon bags, using serum‐free medium supplemented with granulocyte/macrophage‐colony stimulating factor...
Prostate cancer remains one of the most common and deadliest forms cancer, generally respond well to radical prostatectomy associated interventions, up 30% individuals will suffer disease relapse. Although BUB1B was found be essential for cell growth proliferation, even in several kinds tumor cells, specific importance mechanistic role prostate remain unclear.Quantitative Real-Time PCR Western-blot were used detection mRNA protein expression. Lentivirus infection overexpression or knock down...
Altered expression of microRNA (miRNA) is associated with the occurrence and metastasis various tumors. We previously found that miR-218 inhibits tumor angiogenesis through RICTOR/VEGFA axis in prostate cancer (PCa). In this study, we determined also had a negative effect on cell growth, migration, invasion ability PCa. Our data showed bound to Grb2-associated binding protein 2 (GAB2) 3'-UTR region inhibited GAB2 expression. As novel downstream target miR-218, has been reported be involved...
MicroRNA (miRNA) is a class of non‑coding single‑stranded RNA, able to regulate tumor‑associated genes via binding the 3'‑UTR target gene mRNA. Previous publications have demonstrated that miRNA‑218 (miR‑218) acts as tumor‑suppressive miRNA in various types human cancer, including prostate cancer (PCa). However, role miR‑218 regulating PCa cell stemness and epithelial‑mesenchymal transition remains unknown requires further research. In present study, it was downregulated 2 lines could...
BACKGROUND:The aim of this study was to compare the clinical safety and effectiveness transurethral bipolar plasmakinetic enucleation prostate (PKEP) vs. resection (PKRP) in treatment benign hyperplasia (BPH) more than 80 ml. MATERIAL AND METHODS:From June 2015 February 2019, 179 BPH patients with volume greater ml were enrolled separated into a PKEP (n=81) group PKRP (n=98). The 2 groups followed up for 6 months. We collected analyzed data from international Prostate Symptom Score (IPSS),...
Enriched CD34(+) peripheral blood progenitor cells (PBPC) are frequently used as stem cell support in cancer patients following high dose therapy. Since precursor dendritic (DCs) originate from haematopoietic cells, purified might also serve starting for ex- vivo production of DC. In the present study we developed a clinical grade procedure DC derived enriched cells. Different concentrations were grown gas-permeable Teflon bags with different serum-free and serum-containing media...
The aim of this study was to explore the value prostate-specific antigen (PSA) levels, ratio free PSA total (fPSA/TPSA), density (PSAD), digital rectal examination (DRE), transrectal prostate ultrasound (TRUS), and multiparameter MRI (MP-MRI) in differential diagnosis benign prostatic hyperplasia (BPH) cancer (PCa).From February 2016 September 2019, data from 620 patients who underwent systematic ultrasound-guided biopsy (STURS-PB) our hospital were retrospectively collected, including...
Renal cell carcinoma (RCC) is one of the most common malignant cancers in adult urinary system worldwide. Tumor angiogenesis a critical process during cancer progression, as it modulates carcinogenesis and metastasis. In recent years, microRNA‑218 (miR‑218) has been confirmed to play crucial role tumor suppression. However, miR‑218 RCC remains unclear. present study, was found that expression decreased tissues lines detected by real‑time PCR analysis. Tube formation assays migration also...
The aim of this study was to assess the immunogenic potential irradiated lymphoma cells in vivo and determine whether immunogenicity can be enhanced by modulation host immune system. Syngeneic murine models ex were used as an orthotopic cellular vaccination prior challenge with viable tumor cells. We demonstrate that are poorly protective anti-tumor CD8 T-cell responses require addition immunostimulatory monoclonal antibody adjuvant, increased frequency antigen exposure multiple...
Background: Colorectal cancer (CRC) is one of the leading causes cancer-related death, and up to 50% individuals will suffer relapse. Although transmembrane immunoglobulin domain-containing protein 1 (TMIGD1) was found be a protective factor in several renal intestinal diseases, specific role TMIGD1 CRC remains unclear. Objective: To determine tumor suppressor expression its function inhibiting CRC. Methods: We analyzed three Gene Expression Omnibus (GEO) datasets through GEO2R online tool...
Background: Prostate cancer is one of the most commonly diagnosed cancers and common causes cancer-related deaths among men worldwide. Patients who are with localized prostate treated radical prostatectomy often respond well to therapy. The current standard therapy for involves maximal surgical resection, followed by radiotherapy chemotherapy. Clarifying molecular mechanism tumor proliferation recurrence becomes more important clinical therapies cancer. Methods: Quantitative Real-Time PCR...
The development of prostate cancer is complicated and involves a number tumor-associated gene expression level abnormalities. Gene chip technology high-throughput method that can detect levels in different tissues cells on large scale. In the present study, was used to screen differentially expressed genes PC-3 human following GRB-associated binding protein 2 (GAB2) knockdown, corresponding biological information analyzed investigate role GAB2 cancer. cell knocked out hybridization...