A5075766769- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Fungal Infections and Studies
- Curcumin's Biomedical Applications
- Natural product bioactivities and synthesis
- Glycosylation and Glycoproteins Research
- NF-κB Signaling Pathways
- Adenosine and Purinergic Signaling
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Folate and B Vitamins Research
- Genomics, phytochemicals, and oxidative stress
- Neuroinflammation and Neurodegeneration Mechanisms
- Nutritional Studies and Diet
- Synthesis and biological activity
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Biochemical and Molecular Research
- Radiopharmaceutical Chemistry and Applications
- Tryptophan and brain disorders
- T-cell and B-cell Immunology
- Respiratory Support and Mechanisms
- IL-33, ST2, and ILC Pathways
- Diet and metabolism studies
- Monoclonal and Polyclonal Antibodies Research
University Health Network
2019-2020
Krembil Research Institute
2019-2020
University of Oklahoma Health Sciences Center
2009-2019
Pharmaceutical Biotechnology (Czechia)
2012
Chiba University
2010
The novel coronavirus SARS-CoV-2 has become a global health concern. morbidity and mortality of the potentially lethal infection caused by this virus arise from initial viral subsequent host inflammatory response. latter may lead to excessive release pro-inflammatory cytokines, IL-6 IL-8, as well TNF-α ultimately culminating in hypercytokinemia ("cytokine storm"). To address immuno-inflammatory pathogenesis, multiple clinical trials have been proposed evaluate anti-inflammatory biologic...
The accumulation and deposition of β-amyloid (Aβ) is one postulated cause Alzheimer's disease (AD). In addition to its direct toxicity on neurons, Aβ may induce neuroinflammation through the concomitant activation microglia. Emerging evidence suggests that microglia-mediated plays an important role in pathogenesis AD. As brain macrophages, microglia engulf misfolded-Aβ by phagocytosis. However, accumulated toxic paradoxically "hyper-activate" into a neurotoxic proinflammatory less...
Abstract Synthetic curcuminoid EF24 was studied for its effect on the maturation and inflammatory response in murine bone marrow derived immortalized JAWS II dendritic cells (DCs). reduced expression of LPS-induced MHC class II, CD80 CD86 molecules. It also abrogated appearance dendrites, a typical characteristic mature DCs. These effects were accompanied by inhibition activation transcription factor nuclear kappa-light-chain enhancer activated B (NF-κB). Simultaneous reduction...
Abstract Background Coccidioidomycosis or Valley fever is caused by a highly virulent fungal pathogen: Coccidioides posadasii immitis . Vaccine development against of contemporary interest because large number relapses and clinical failures are reported with antifungal agents. An efficient Th1 response engenders protection. Thus, we have focused on developing dendritic cell (DC)-based vaccine for coccidioidomycosis. In this study, investigated the immunostimulatory characteristics an...
Unresolved and prolonged inflammation is a pathological basis of many disorders such as cancer multiple organ failure in shock. Interleukin-1 receptor (IL-1R) superfamily consists IL-1R1 pathogen pattern recognition toll-like receptor-4 (TLR4) which, upon ligand binding, initiate pro-inflammatory signaling. The study objective was to investigate the effect diphenyldifluoroketone EF24 on expression TLR4 lipopolysaccharide (LPS)-stimulated dendritic cells (DCs). Immortalized murine bone...
Severe blood loss in victims of trauma creates an exaggerated inflammatory background that contributes to the development intravascular coagulopathy and multiple organ dysfunction syndrome. We hypothesized treatment with diphenyldifluoroketone EF24, inhibitor nuclear factor kappa-B, would have salutary effects hemorrhagic shock. The objective this study was investigate effect EF24 on expression interleukin-1 receptor (IL-1R) superfamily a rat model hypovolemic Hypovolemia induced by...
Abstract The dendritic cells (DCs) have an important role in antigen presentation and regulation of host defense. Immunization with vitro modified DCs has been shown to activate protective immune responses. Among various approaches used for modifying DCs, the nonviral genetic transfection is easy safe as compared viral-mediated methods. In this study, we investigated T cell stimulatory ability nonvirally, gene-transfected primary murine DCs. We isolated bone marrow derived transfected...
Abstract Dendritic cells (DCs) are the most potent antigen-presenting that link antigen-specific innate host responses with adaptive immunity. They recognize pathogens, pathogen-ligands and inflammatory signals via pattern recognition receptors. Inflammatory induce DC maturation release of cytokines, chemokines chemical mediators. We synthesized EF24, a known more analog curcumin, studied its immunomodulatory properties using immortalized murine DCs (JAWS II). hypothesized pharmacological...
Abstract Dendritic cell (DC)-based vaccines have shown promising results in cancer and infectious diseases. Success of the DC-vaccine depends on multiple factors including host genetic background, trafficking through tissues, interaction activation naïve immune cells. We studied immunostimulatory ability BALB/c mice, extremely-susceptible to Coccidioides infection. was prepared by transfecting DCs with a plasmid DNA encoding Coccidioides-Ag2/PRA-cDNA (protective epitope). Secreted...
major amastin antigen in BALB/c mice model.We evaluated the immunogenicity and protective efficacy of plasmid DNA vaccines encoding amastin-Enhanced Green Fluorescent Protein (EGFP) VP22-amastin-EGFP.Optimal cellmediated immune responses were observed immunized with VP22-amastin-EGFP as assessed by cytokine gene expression analysis using real time RT-PCR.Vaccination fusion construct elicited significantly higher IFN-g response upon stimulation splenocytes from compared to administration VP22...
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the ‘Save PDF’ action button.