Abstract Background Whole‐organ engineering provides a new alternative source of donor organs for xenotransplantation. Utilization decellularized whole‐organ scaffolds, which can be created by detergent perfusion, is strategy tissue engineering. In this article, our aim to scale up the decellularization process human‐sized liver and kidney generate matrix with optimal stable characteristics on clinically relevant scale. Methods Whole porcine were perfusion using different detergents (1% SDS...

Abstract Decellularized xenogeneic whole-liver matrices are plausible biomedical materials for the bioengineering of liver transplantation. A common method to reduce inflammatory potential is crosslinking. Nevertheless, a comprehensive analysis immunogenic features cross-linked decellularized tissue still lacking. We aimed immunogenicity porcine matrix through crosslinking with glutaraldehyde or genipin, new natural agent and investigated mechanism immune-mediated responses. The histologic...

Probiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains inhibit C. difficile. This study examined anti-clostridial effects Bifidobacterium longum JDM301 (JDM301), a widely commercial probiotic strain in China, vitro vivo. In evaluation revealed significant reduction counts when was co-cultured with difficile, which correlated...

Abstract The blood–brain barrier (BBB) contributes to the maintenance of brain homeostasis. Gut microbiome composition affects BBB development and expression tight junction proteins in rodents. However, we still do not know if there is any direct effect gut microbial on permeability function normal adult animals. In current study, determined temporal spatial changes rhesus monkeys receiving amoxicillin‐clavulanic acid (AC) by monitoring cerebrospinal fluid/serum albumin ratio volume transfer...

This study aims to evaluate the effectiveness and safety of spheroid reservoir bioartificial liver (SRBAL) with porcine hepatocyte organoids in a preclinical nonhuman primate model acute failure (ALF).Methods: Thirty healthy rhesus monkeys were infused α-amanitin lipopolysaccharide randomized into five groups (ALF alone control group; sham no-cell SRBAL treatment A, B C started at 12 h, 24 h 36 after induction ALF, respectively).Animals continuously treated device for 6 followed up 336...

Three-dimensional (3D) culture via micropattern arrays to generate cellular spheroids seems a promising in vitro biomimetic system for liver tissue engineering applications, such as drug screening. Recently, organ-derived decellularized extracellular matrix emerges arguably the most bioink. Herein, (DLM)-derived array chips were developed fabricate size-controllable and arrangement-orderly HepG2 The porcine DLM was obtained by removal of components then ground into powder, followed...

The immobilization of heparin, a new and versatile approach to the surface modification decellularized tissues, has potential greatly improve hemocompatibility engineered tissue constructs derived from organs. We report on porcine liver scaffolds (DLSs) heparinized by end-point attachment (EPA) technique. DLSs (HEP-DLSs) have ability bind slowly release heparin-binding growth factors. hypothesized that DLS-immobilized heparin acts as an antithrombotic coating reagent binds vascular...

In the present study, we aimed to determine whether combination of aggregate culture and decellularized liver scaffolds (DLSs) promoted hepatic differentiation murine bone marrow-derived mesenchymal stem cells (BM-MSCs) into high yields mature hepatocytes in vitro. Four culturing methods for [single cell (2D), spheroids (3D), 2D + DLS 3D DLS] were studied. To stages MSCs, RT-qPCR hepatocyte genes, immunostaining markers, functional analyses all performed. Compared with other groups,...

This study aims to evaluate ECM-coated micropattern arrays derived from decellularization of native porcine lungs as a novel three-dimensional cell culture platform.ECM was exploited prepare hydrogels. Then, dECM-coated were fabricated at four different diameters (50, 100, 150 and 200 ​μm) using polydimethylsiloxane (PDMS). Two lung cancer lines, A549 H1299, tested on array platform for adhesion, distribution, proliferation, viability, phenotype expression, drug screening the cytotoxicity...

Poly (β-amino ester) (PBAE) is a class of biodegradable polymers containing ester bonds in their main chain, extensively investigated as cationic polymer carriers for siRNA. Most current PBAE rely on termination with hydrophilic or charged amines. In this study, platform consisting 168 hydrophobic alkyl chain terminals constructed through sequential aza-Michael addition. A large number effective are identified vitro screening the siLuc delivery to HeLa-Luc cells. Specifically, PA8-C6 and...

An individualized, tissue-engineered liver suitable for transplanting into a patient with disease would be of great benefit to the and healthcare system. The possess functions original healthy organ. Two fields study, (i) using decellularized tissue as cell scaffolding, (ii) stem differentiation functional cells, are coming together make this concept feasible. scaffolds (DLS) can interact cells promote signal transduction three-dimensional (3D) aggregations maintain phenotypes improve after...

Liver organoids have recently been applied as models for liver disease and drug screening, especially when combined with liver-on-a-chip technologies. Compared to hepatocyte-like cells, primary hepatocytes high functionality but cannot maintain their function cultured in vitro. Mesenchymal stem cells (MSCs) enhance hepatocyte metabolism co-cultured hepatocytes. MSCs can help induced pluripotent generate an organoid structure via the MSC-based traction force triggered by extracellular matrix...

Abstract Successful porcine hepatocyte isolation is crucial for the development of bioartificial liver devices and transplantation. Serva collagenase NB grades are formulated collagenases that suitable various tissue applications. N‐acetylcysteine ( NAC ) can improve viability human hepatocytes. The aim this study was to compare effectiveness two effect on from tissue. Porcine hepatocytes were isolated using perfusion method Bama mini pigs assigned 4 group (n=6), 8 or 8+ (n=6). Viability...

One of the key steps using CRISPR/Cas9 to obtain gene-edited cells used in generating animals combined with somatic cell nuclear transplantation (SCNT) is harvest monoclonal genetic modifications. However, primary as donors always grow slowly and fragile after a series gene-editing operations. The extracellular matrix (ECM) formulated directly from different organs comprises complex proteins growth factors that can improve regulate cellular functions cells. Herein, sodium lauryl ether...

The development of novel culture systems that mimic the in vivo microenvironment may be beneficial for inducing differentiation stem cells and promoting liver function. In present study, spheroid cultures decellularized scaffolds (DLSs) were utilized to obtain differentiated hepatocyte‑like cells. Mouse bone marrow (BM)‑derived mesenchymal (MSCs) self‑aggregated into spheroids under low‑attachment conditions implanted DLSs via a negative pressure suction device. Albp‑ZsGreen adenoviral...

The challenge of using bioengineered liver lies in sustaining the quantity high-quality hepatocytes and vasculature for blood perfusion. We characterized heparinization a porcine decellularized scaffold (DLS) as carrier to support hepatocyte angiogenesis, thereby developing functional vascularized hepatic tissue useful treat injury.The DLS was obtained by removal cellular components then subjected end-point attachment technique. heparinized DLSs were recellularized with rat spheroids...

Genetic constructs with promoters fused to reporter genes for simultaneous monitoring of cellular events have been the focus attention in recent years. Adenoviral vectors, which distinctive characteristics, used monitor differentiation stem cells vitro. In present study, a modified adenoviral vector was constructed, containing mouse, rat, and human general albumin promoter sequence ZsGreen gene, evaluated its efficiency different cell types. Two hepatocyte lines (Hepa1-6 HepG2), rat primary...

Objective: To evaluate the efficacy and safety of cell sheets containing allogeneic keratinocytes fibroblasts in treatment partial-thickness burn wounds. Methods: The were constructed using polyurethane biofilm as carrier. Then gross observation histological conducted. From April 2016 to December 2017, Changhai Hospital Naval Medical University recruited patients with acute wounds that met inclusion criteria for this prospective positively self-controlled clinical trial. Recruitment 40...

The traditional user behavior analysis method lacks the support of big data technology and demands are not subdivided, which causes mismatch marketing demands.In view different standards mobile users, personalized services have been accurately customized.At same time, network users based on Hadoop, Hive, Zookeeper other technologies has proposed.Based this method, system including download program module call bill log, processing classification scheduler task statistics storage result...