A5087929637- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Gastrointestinal motility and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Biofuel production and bioconversion
- Adipose Tissue and Metabolism
- Probiotics and Fermented Foods
- RNA modifications and cancer
- Fungal and yeast genetics research
- Hepatitis B Virus Studies
- Tryptophan and brain disorders
- Cancer-related molecular mechanisms research
- Polysaccharides and Plant Cell Walls
- Immune Response and Inflammation
- Microbial Metabolic Engineering and Bioproduction
- Inflammasome and immune disorders
- NF-κB Signaling Pathways
- Diet and metabolism studies
- Reproductive System and Pregnancy
- Diabetes Treatment and Management
- Enzyme Production and Characterization
- Galectins and Cancer Biology
- Circular RNAs in diseases
- Geomagnetism and Paleomagnetism Studies
Xuzhou Medical College
2016-2024
Chongqing Medical University
2020
Shenzhen University
2020
Shandong University
2011-2014
State Key Laboratory of Microbial Technology
2014
Proper perception of the extracellular insoluble cellulose is key to initiating rapid synthesis cellulases by cellulolytic Trichoderma reesei. Uptake soluble oligosaccharides derived from hydrolysis represents a potential point control in induced cascade. In this study, we identified major facilitator superfamily sugar transporter Stp1 capable transporting cellobiose reconstructing assimilation system Saccharomyces cerevisiae. The absence T. reesei resulted differential response Avicel...
Abstract Antibiotic-induced dysbiosis is a major risk factor for Clostridioides difficile infection (CDI), and fecal microbiota transplantation (FMT) recommended treating CDI. However, the underlying mechanisms remain unclear. Here, we show that Tritrichomonas musculis ( T.mu ), an integral member of mouse gut commensal microbiota, reduces CDI-induced intestinal damage by inhibiting neutrophil recruitment IL-1β secretion, while promoting Th1 cell differentiation IFN-γ which in turn enhances...
ABSTRACT Appropriate perception of cellulose outside the cell by transforming it into an intracellular signal ensures rapid production cellulases cellulolytic Hypocrea jecorina . The major extracellular β-glucosidase BglI (CEL3a) has been shown to contribute efficient induction cellulase genes. Multiple β-glucosidases belonging glycosyl hydrolase (GH) family 3 and 1, however, exist in H. Here we demonstrated that CEL1b, like CEL1a, was displaying vitro transglycosylation activity. We then...
Th17 cells and interleukin-17 (IL-17) have been found to play an important role in the pathology of multiple sclerosis (MS) its animal model, experimental autoimmune encephalomyelitis (EAE). Response IL-17, reactive astrocytes accompany with immune infiltration axonal damage MS/EAE. However, regulatory mechanism IL-17-activated inflammation EAE process still remain largely unknown. Here, we elucidated that miR-409-3p miR-1896, as co-upregulated microRNAs activated mice, targeted suppressor...
Probiotics might offer an attractive alternative to prevent and control Clostridium difficile (C. difficile) infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains inhibit C. difficile. This study examined anti-clostridial effects Bifidobacterium longum JDM301 (JDM301), a widely commercial probiotic strain in China, vitro vivo. In evaluation revealed significant reduction counts when was co-cultured with difficile, which correlated...
Lactose (1,4-O-β-d-galacto-pyranosyl-d-glucose) induces cellulolytic enzymes in Trichoderma reesei and is fact one of the most important soluble carbon sources used to produce cellulases on an industrial level. The mechanism underlying induction is, however, not fully understood. In this study, we investigated cellular functions intracellular β-glucosidases CEL1a CEL1b cellulase genes by lactose T. reesei. We demonstrated that while were functionally equivalent mediating induction,...
It is considered that intestinal barrier dysfunction and systemic endotoxemia drive obesity its related complications. However, what causes remains to be elucidated. Here, we showed the gut microbiota from high-fat diet (HFD)-fed mice had impaired ability degrade dietary flavonoids, in correspondence, microbial-derived flavonoid metabolite desaminotyrosine (DAT) was reduced. Supplementation of DAT drinking water able counter HFD-induced body fat mass accumulation weight increment. This...
Background: Obesity is characterized by increased adipose tissue mass that results from fat cell size (hypertrophy) and number (hyperplasia). The molecular mechanisms govern the regulation differentiation of adipocytes play a critical role for better understanding pathological mechanism obesity. However, adipocyte still unclear. Objective: present study aims to compare gene expression changes during in transcriptomic level, which may help understand differentiation. Methods: RNA sequencing...
Interleukin-7 receptor (IL-7R) is involved in the abnormal function of solid tumors, but role and regulatory mechanisms IL-7R HBV-related hepatocellular carcinoma (HCC) are still unclear.Gene protein expression levels were examined hepatoma cells transfected with hepatitis B virus (HBV) plasmids multifunctional nonstructural X (HBX). The HBX was measured by immunohistochemical analysis HCC tissues. NF-κB Notch1 pathways HBX-mediated examined. Activation downstream intracellular signaling...
Our current understanding of the host-microbiota interaction in gut is dominated by studies focused primarily on prokaryotic bacterial communities. However, there an underappreciated symbiotic eukaryotic protistic community that integral part mammalian microbiota. How commensal protozoan bacteria might interact to form a stable microbial remains poorly understood. Here, we describe murine commensal, phylogenetically assigned as Tritrichomonas musculis, whose colonization resulted reduction...
Current evidence to support extensive use of probiotics in inflammatory bowel disease is limited and factors that contribute the inconsistent effectiveness clinical probiotic therapy are not completely known. Here, we used Bifidobacterium longum JDM 301 as a model study potential may influence effect experimental colitis. We found B. tempering colitis varied across individual mice even with same genetic background. The efficacy was highly correlated host gut microbial community features....
The physiologic signals that regulate beige adipogenesis remain incompletely understood, especially those limit browning and prevent overexpenditure of energy. In this study, the TNF family member cytokine lymphotoxin-like inducible protein competes with glycoprotein D for herpesvirus entry on T cells (LIGHT), also known as super 14 (TNFSF14), can inhibit adipose precursor differentiation into adipocytes. acute cold stress, LIGHT deficiency in mice accelerated subcutaneous white tissue...
Background/Aims: Multiple sclerosis (MS) is an autoimmune disease in the central nervous system associated with demyelination and axonal injury. Astrocyte activation involved pathogenesis of MS experimental encephalomyelitis (EAE), animal model MS. This study was designed to find potential lncRNAs EAE mice activated astrocytes. Methods: we performed microarray analysis from brain tissues primary mouse astrocytes treated IL-9(50 ng/ml). 12 were validated through real-time PCR. Gene ontology...
The metabolic alterations of amino acid metabolism are closely associated with inflammatory response. However, relatively little is known about the roles phenylalanine (Phe)/tyrosine (Tyr) catabolites during inflammation. Nitisinone (NTBC) an orphan drug used to treat hereditary tyrosinemia type I potentially by changing Phe/Tyr flow. In this study, we NTBC as a tool investigate potential role catabolic pathway in responses. We found that was effective tempering bacterial endotoxin...
Background Toxoplasmosis affects a quarter of the world’s population. Toxoplasma gondii ( T . ) is an intracellular parasitic protozoa. Macrophages are necessary for proliferation and spread by regulating immunity metabolism. Family with sequence similarity 96A (Fam96a; formally named Ciao2a) evolutionarily conserved protein that highly expressed in macrophages, but whether it play role control infection unknown. Methodology/principal findings In this study, we utilized myeloid cell-specific...
ABSTRACT Delta‐like homolog 2 (DLK2) plays a crucial role in adipogenesis, chondrogenic differentiation, and the progression of certain cancers. However, key roles DLK2 underlying hepatocellular carcinoma (HCC) remain ambiguous. In current study, we demonstrate that is upregulated HCC, significantly correlated with clinicopathological variables serves as an independent diagnostic marker. Functional assays reveal facilitates malignant HCC vitro vivo models. Mechanistically, binds to EGFR...
Although hepatitis B virus (HBV) infection is responsible for liver cancer, the exact mechanism of its action remains unclear. μ1 adaptin an intrinsic part clathrin adaptor AP-1 complex. In addition to canonical biological function that involves cargo sorting and vesicular transport, recent studies have demonstrated participates in cell growth proliferation. The aim present study was investigate effects complex subunit mu-1 (AP1M1) on cancer reports first time AP1M1 upregulated...
LIM and SH3 domain protein 1 (LASP‐1) is known to participate in the progression of hepatocellular carcinoma (HCC). We previously showed that ectopic expression hepatitis B virus (HBV) X (HBX) enhanced LASP‐1, which promoted proliferation migration HCC cells. Here, we further demonstrated molecular mechanism underlying upregulation mediated by HBX, HBV‐infected Through a luciferase activity assay, discovered LASP‐1 promoter region regulated HBX contained an AP‐1 binding element human...
Gluconolactone, an inhibitor of β-glucosidase, is capable inducing cellulase gene expression in cellulolytic filamentous fungus<italic>Trichoderma reesei</italic>.
The magnetotactic bacterium Magnetospirillum magneticum AMB-1 can grow at variable oxygen concentrations, although the intracellular magnetic structures, magnetosomes, are only synthesized under microaerobic or anaerobic conditions. Three members of peroxiredoxin family were identified in M. AMB-1. All purified recombinant proteins displayed thiol-dependent peroxidase activities. Allelic replacement mutagenesis revealed that, absence three genes had no effect on either growth formation...
Abstract LIM and SH3 domain protein (LASP-1) is responsible for the development of several types human cancers via interaction with other proteins; however, precise biological functions proteins interacting LASP-1 are not fully clarified. Although role in hepatocarcinogenesis has been reported, implication interactors HBV-related hepatocellular carcinoma (HCC) clearly evaluated. We obtained information regarding from public databases published studies. Via bioinformatics analysis, we found...
Aurora-A kinase, a serine/threonine mitotic kinase involved in mitosis, is overexpressed several human cancers.A recent study showed that mediates glucose metabolism via SOX8/FOXK1 ovarian cancer.However, the roles of metabolic diseases remain unclear.This found Aurka loss intestinal epithelium promoted age-induced obesity and enlargement lipid droplets parallel with an increase infiltrated macrophages white adipocyte tissue (WAT) male mice.Moreover, induced expression regulatory genes,...
Recent progress indicates that the gut microbiota plays important role in regulating host's glucose homeostasis. However, mechanisms remain unclear. Here, we reported one integral member of murine microbiota, protozoan Tritrichomonas musculis could drive metabolic imbalance. Using metabolomics analysis and vivo assays, found mechanistically this influences host metabolism by facilitating production a significant amount free choline. Free choline be converted sequentially choline-utilizing...