A5019853124- Veterinary Oncology Research
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- Gut microbiota and health
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Environmental Science and Water Management
- Microbial infections and disease research
- Environmental Science and Technology
- Glioma Diagnosis and Treatment
- AI in cancer detection
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Cancer Genomics and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Lung Cancer Diagnosis and Treatment
- Cutaneous Melanoma Detection and Management
- Cell Image Analysis Techniques
National Cancer Institute
2015-2025
National Institutes of Health
2018-2025
Center for Cancer Research
2018-2025
National Cancer Institute
2024
Purdue University West Lafayette
2014-2022
University of Georgia
2013-2014
Abstract Background Lung cancer is the leading diagnosis worldwide and number one cause of deaths. Exposure to cigarette smoke, primary risk factor in lung cancer, reduces epithelial barrier integrity increases susceptibility infections. Herein, we hypothesize that somatic mutations together with smoke generate a dysbiotic microbiota associated carcinogenesis. Using tissue from 33 controls 143 cases, conduct 16S ribosomal RNA (rRNA) bacterial gene sequencing, RNA-sequencing data cases The...
The National Cancer Institute-led multidisciplinary Comparative Brain Tumor Consortium (CBTC) convened a glioma pathology board, comprising both veterinarian and physician neuropathologists, conducted comprehensive review of 193 cases canine glioma. immediate goal was to improve existing classification methods through creation histologic atlas features, thus yielding greater harmonization phenotypic characterization. long-term support future incorporation clinical outcomes genomic data into...
Cellular senescence and the senescence-associated secretory phenotype (SASP) may contribute to development of radiation therapy–associated side effects in lung blood vessels by promoting chronic inflammation. In brain, inflammation contributes neurologic disease, including Alzheimer's disease. this study, we investigated roles cellular Δ133p53, an inhibitory isoform p53, radiation-induced brain injury. Senescent cell types irradiated human were identified with immunohistochemical labeling...
Abstract Background Osteosarcoma (OS) is a malignant bone tumor that often develops during the period of rapid growth associated with adolescence. Despite successful primary control accompanied by adjuvant chemotherapy, death from pulmonary metastases occurs in approximately 30% patients within 5 years. As overall survival remains unchanged over last 30 years, urgent needs for novel therapeutic strategies exist. Cancer metastasis characterized complex molecular events which result...
Abstract Canine osteosarcoma is increasingly recognized as an informative model for human osteosarcoma. Here we show in one of the largest clinically annotated canine transcriptional datasets that two previously reported, well de novo gene signatures devised through single sample Gene Set Enrichment Analysis (ssGSEA), have prognostic utility both and patients. Shared molecular pathway alterations are seen immune cell signaling activation including TH1 TH2 signaling, interferon inflammatory...
Tau accumulation is a core component of Alzheimer’s disease and other neurodegenerative tauopathies. While tau’s impact on neurons major area research, the effect extracellular tau astrocytes largely unknown. This article summarizes our recent studies showing that astrocyte senescence plays critical role in diseases integrates into regulatory loop senescent astrocyte-mediated neurotoxicity. Human vitro undergoing were shown to acquire inflammatory senescence-associated secretory phenotype...
ABSTRACT Osteosarcoma is the most common malignant bone tumor in young patients and remains a significant clinical challenge, particularly context of metastatic disease. Despite extensive documentation genomic alterations osteosarcoma, studies detailing immunosuppressive mechanisms within osteosarcoma microenvironment are lacking. Our objective was to characterize spatial transcriptional landscape reveal these identify promising therapeutic targets. Here, we performed profiling on cohort...
Osteosarcoma is the most common malignant bone tumor in young patients and remains a significant clinical challenge, particularly at metastatic stage. Studies detailing immunosuppressive mechanisms within osteosarcoma microenvironment are needed to elucidate cellular communities that support growth identify therapeutic approaches target crosstalk between cancer cells microenvironment. Here, we performed spatial transcriptional profiling on cohort of pulmonary metastases from pediatric...
Background: Determining mitotic index by counting figures (MFs) microscopically from tumor areas with most abundant MF (hotspots [HS]) produces a prognostically useful grading biomarker. However, interobserver concordance identifying and HS can be poorly reproducible. Immunolabeling MF, coupled computer-automated image analysis, improve reproducibility. A computational system for obtaining values across digitized whole-slide images (WSIs) was sought that would minimize impact of artifacts,...
Validating digital pathology as substitute for conventional microscopy in diagnosis remains a priority to assure effectiveness. Intermodality concordance studies typically focus on achieving the same by display of whole slide images and microscopy. Assessment discrete histological features images, such mitotic figures, has not been thoroughly evaluated diagnostic practice. To further gauge interchangeability with primary diagnosis, 12 pathologists examined 113 canine naturally occurring...
Abstract Osteosarcoma is a highly metastatic primary bone tumour that occurs spontaneously in both pet dogs and humans. Patterns of metastasis to organs beyond the most common site (lung) are poorly characterised it unknown whether specific associations between patterns progression patient features exist. This retrospective study necropsy findings 83 receiving standardised therapy clinical monitoring prospective trial setting document correlate outcomes with these other tumour‐specific...
Following preoperative chemotherapy of 9-18 weeks duration limb salvage procedures were performed instead ablative surgery in about 1/2 the patients (pts). Overall continuous disease-free survival rate is 69% (80/115) at 37 (21-51) months. 5 pts died from therapy related complications, 4 developed a local failure (2 following amputation and 2 each) 26 pulmonary metastases. The incidence metastases after en bloc resection, but not shank rotation plasty, was found to be significantly increased...
Thyroid transcription factor-1 (TTF-1) is a specific and sensitive marker for canine pulmonary tumors but also expressed in thyroid carcinomas, which commonly metastasize to lung. Napsin A surfactant protein (SP-A) are used the histologic diagnosis of non-small-cell lung cancer humans have not been thoroughly evaluated neoplasms dogs. The objective this study was compare efficacy immunohistochemistry SP-A, napsin A, TTF-1 carcinomas. TTF-1, SP-A antibodies were applied 67 formalin-fixed,...
Abstract Development of animal models that accurately recapitulate human cancer is an ongoing challenge. This particularly relevant in the study osteosarcoma (OS), a highly malignant bone tumor diagnosed approximately 1000 pediatric/adolescent patients each year. Metastasis leading cause patient death underscoring need for metastatic OS. In this study, we describe how existing OS mouse can be interrogated time-course context to determine kinetics spontaneous metastasis from orthotopically...
The metabolic dependencies of cancer cells have substantial potential to be exploited improve the diagnosis and treatment cancer. Creatine riboside (CR) is identified as a urinary metabolite associated with risk prognosis in lung liver However, source high CR levels patients well their implications for these aggressive cancers remain unclear. By integrating multiomics data on cancer, we shown that cell-derived metabolite. Global metabolomics gene expression analysis human tumors matched...
Osteosarcomas (OSs) are aggressive bone tumors with many divergent histologic patterns. During pathology review, OSs subtyped based on the predominant pattern; however, often demonstrate multiple This high tumor heterogeneity coupled scarcity of samples compared other types render histology-based prognosis challenging. To combat lower case numbers in humans, dogs spontaneous have been suggested as a model species. Herein, convolutional neural network was adversarially trained to classify...
Following publication of the original paper [1], authors submitted a new Additional file 5 to replace one containing formatting issues. The updated is published in this correction.
Abstract Osteosarcoma is a relatively rare but aggressive cancer of the bones with shortage effective biomarkers. Although less common in humans, Osteosarcomas are fairly adult pet dogs and have been shown to share many similarities their human analogs. In this work, we analyze bulk transcriptomic data 213 primary 100 metastatic samples from 210 enrolled nation-wide clinical trials uncover three Tumor Microenvironment (TME)-based subtypes: Immune Enriched (IE), Dense Extra-Cellular...