Expression of the transmembrane-4-L-six-family-1 (TM4SF1) is high in human pancreatic cancer cells, but underlying mechanism remains unclear. In this study, we aimed to identify and characterize microRNAs that regulate TM4SF1 expression PC cells. Western blot analysis quantitative polymerase chain reaction were used detect hsa-miR-141 levels four cell lines. SW1990 BxPc-3 cells transfected with inhibitor miR-141, negative control, miR-141 mimic control; invasion, migration, proliferation,...

Drought stress inhibits the development of maize ears. Abscisic acid (ABA) is a plant hormone that can regulate physicology metabolism under abiotic stress. In this study, varieties Zhengdan 958 (ZD958) and Xianyu 335 (XY335) with different filling stages were used as materials. Three treatments set in period: normal irrigation (CK), drought (stress); exogenous ABA + (ABA+stress). They to study physiological regulation on ears during Exogenous inhibited bald tip decline biomass, increased...

MicroRNAs have emerged as crucial regulators of tumorigenesis. However, the mechanism by which miR‑203 is involved in pathogenesis pancreatic cancer (PC) remains elusive. In present study, PC cell lines were used an experimental model to investigate expression and functional role PC. mimic virus, miRNA negative control virus Survivin shRNA transfected into line, CFPAC‑1. mRNA protein levels detected using qPCR western blot analysis. Proliferation, apoptosis cycle profiles MTT assay flow...

MicroRNAs are a class of small noncoding RNA which play important regulatory roles in variety cancers. MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, we combined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulated with computational techniques the sera ovarian cancer patients while using healthy serum as control. Among above, four (miR-22, miR-93, miR-106b, miR-451) were validated by...

MicroRNAs (miRNAs) are non-coding, single-stranded RNAs of approximately 22 nt and constitute a novel class gene regulators that found in both plants animals. Several studies have demonstrated serum miRNAs could serve as potential biomarkers for the detection various cancers other diseases. A few documents regarding stability liver cancer-related available. systemic analysis miRNA is quite necessary. The purpose this study was to evaluate from three different sources, cultured cancer Huh-7...

Background: Transmembrane-4-L-six-family-1 (TM4SF1) functions to regulate cell growth and mobility TM4SF1 expression was upregulated in pancreatic cancer. This study further investigated the role of regulating cancer epithelial-mesenchymal transition (EMT) angiogenesis underlying molecular events.Methods: Tissue specimens were collected from 90 patients for immunohistochemical qRT-PCR analysis miR-141 levels, respectively. Pancreatic lines used vitro assays, while nude mice vivo...

Pancreatic cancer is a highly malignant neoplastic disease of the digestive system. In present study, dataset GSE62165 was downloaded from Gene Expression Omnibus (GEO) database. contained data 118 pancreatic ductal adenocarcinoma samples (38 early‑stage tumors, 62 lymph node metastases and 18 advanced tumors) 13 control samples. Differences in expression levels genes between normal tissues tumors were investigated. A total 240 differentially expressed (DEGs) identified using R software 3.5...

Candida albicans is among the most prevalent invasive fungal pathogens for immunocompromised individuals and novel therapeutic approaches that involve immune response modulation are imperative. Absent in melanoma 2 (AIM2), a pattern recognition receptor DNA sensing, well recognized its involvement inflammasome formation crucial role safeguarding host against various pathogenic infections. However, of AIM2 defense C. infection remains uncertain. This study reveals gene expression induced...

Streptococcus mutans (S. mutans) is one of the primary pathogens responsible for dental caries. gordonii gordonii) early colonizers plaque and can compete with S. growth. In present analysis, we explored key target genes against in using 80 clinical isolates varying capabilities gordonii. A principal coordinate analysis revealed significant genetic diversity differences between antagonistic non-antagonistic groups. Genomic comparisons 33 61 that were, respectively, positively negatively...

Background Gastric cancer (GC) is a prevalent with high mortality and strong invasiveness, the entire regulatory networks of GC still unclear. Objective The aim this study was to explore specific mechanism effect nucleolar protein 6 (NOL6) on proliferation apoptosis cells. Methods human gastric adenocarcinoma cell line HGC-27 AGS were cultured. qRT-PCR used verify expression level NOL6 in cells; MTT EdU test proliferation; TUNEL staining Flow cytometry detect apoptosis; downstream genes...

Abstract Background Mammary Pathogenic Escherichia coli (MPEC) is an important pathogen that can escape the attack of host immune system through biofilm formation and proliferate in mammary gland continuously, resulting mastitis cows causing enormous economic losses. As effector AI-2 quorum sensing, LsrR extensively affects expression levels hundreds genes related to multiple biological processes model E. strain. However, regulatory role MPEC whether it involved pathogenesis has been seldom...

The composite score for insulin resistance (IR), known as the Metabolic Score of Insulin Resistance (METS-IR), serves an assessment tool IR and has been previously linked to symptomatic intracranial hemorrhage poor functional outcomes in patients with acute ischemic stroke (AIS). Despite these associations, impact METS-IR on early neurological deterioration (END) minor AIS who underwent intravenous administration recombinant tissue-type plasminogen activator (IV-rtPA) remains inadequately...

Serine/threonine-protein kinase-1 (SMG-1) belongs to the phosphatidylinositol 3‑kinase‑related kinase family. Altered expression of SMG-1 contributes human carcinogenesis and cancer progression. The present study detected levels in normal cancerous pancreatic tissues then assessed effects SMG-1-knockdown cell lines vitro. A tissue array were used detect a lentivirus expressing either or negative control short hairpin (sh)RNA knock down lines. Western blot, proliferation, Cell Counting kit-8,...

Abstract Purpose Ectopic fat accumulation plays a significant role in obesity-related metabolic dysfunction, and few studies have reported an association between ectopic gastric risk factors. We aim to fulfill this need by assessing the degree of submucosal pathologic sections 190 sleeve gastrectomy specimens. Methods Study patients were divided into two groups (D1 D2) based on whether exceeded 1/3 submucosa stomach. Demographic factors compared groups. Metabolic variables that might be...

Abstract Background Mammary Pathogenic Escherichia coli (MPEC) is an important pathogen that can escape the attack of host immune system through biofilm formation and proliferate in mammary gland continuously, resulting mastitis cows causing enormous economic losses. As effector AI-2 quorum sensing, LsrR extensively affects expression levels hundreds genes related to multiple biological processes model E. strain. However, regulatory role MPEC whether it involved pathogenesis has been seldom...

Abstract Background Streptococcus mutans ( S. ) is an important pathogenic bacterium that causes dental caries, while gordonii a non-cariogenic inhibits the growth of . The SepM protein can promote inhibitory ability against by cleaving CSP-21 and activating ComDE two-component system. This study was designed to explore sepM mutation in clinical isolates related function regulation interactions with Methods strains inhibit constitute group. 286 C-serotype were categorized into n = 114)...