A5100673921- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Animal Virus Infections Studies
- RNA Research and Splicing
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Chromosomal and Genetic Variations
- Prostate Cancer Treatment and Research
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Prostate Cancer Diagnosis and Treatment
- Ovarian cancer diagnosis and treatment
- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- Statistical Methods in Clinical Trials
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- Soil erosion and sediment transport
- Immune Response and Inflammation
- Genetic Mapping and Diversity in Plants and Animals
- Estrogen and related hormone effects
- Landslides and related hazards
- Microbial infections and disease research
Tongji Hospital
2024
Huazhong University of Science and Technology
2024
University of Hong Kong
2022
Sichuan Animal Science Academy
2015-2021
Second Affiliated Hospital of Nanjing Medical University
2020
China Medical University
2019
Fourth Affiliated Hospital of Harbin Medical University
2015
Harbin Medical University
2015
First Affiliated Hospital of Fujian Medical University
2013
Fujian Medical University
2013
How often micro-RNA (miRNA) genes emerged and how fast they evolved soon after their emergence are some of the central questions in evolution miRNAs. Because most known miRNA ancient highly conserved, these can be best answered by identifying newly genes. Among 78 Drosophila reported before 2007, only 5 confirmed to genus (although many more found data set; e.g., Ruby et al. 2007; Stark Lu 2008). These new have undergone numerous changes, even normally invariant mature sequences. Four them...
Toll-like receptor 4 (TLR4)-tumor necrosis factor 6 (TRAF6) signaling is activated in atherosclerosis (AS), inducing inflammatory mediators. Because miR-146a, a TLR4 microRNA (miRNA), can regulate during responses, this study investigated the effects of aerobic exercise on TLR4-targeted miRNAs AS. Apolipoprotein E-null mice fed high-fat diet for 12 weeks were separated into 3 groups: (i) no treatment (ii) statin (AD), or (iii) (AE). Plaques and foam cells observed untreated control groups,...
The tumor protein D52 (TPD52) is an oncogene overexpressed in breast cancer. Although the oncogenic effects of TPD52 are well recognized, how its expression and role migration/invasion still not clear. This study tried to explore regulative microRNA-34a (miR-34a), a suppressive miRNA, on miR-34a was found significantly decreased cancer specimens with lymph node metastases cell lines. clinicopathological characteristics analyzed showed that lower levels were associated advanced clinical...
Growth performance and meat quality are important traits for the pig industry consumers. Adipose tissue is main site at which fat storage fatty acid synthesis occur. Therefore, we combined high-throughput transcriptomic sequencing in adipose muscle tissues with quantification of corresponding phenotypic features using seven Chinese indigenous breeds one Western commercial breed (Yorkshire). We obtained data on 101 traits, from principal component analysis distinguished two groups: associated...
Background: Transmembrane-4-L-six-family-1 (TM4SF1) functions to regulate cell growth and mobility TM4SF1 expression was upregulated in pancreatic cancer. This study further investigated the role of regulating cancer epithelial-mesenchymal transition (EMT) angiogenesis underlying molecular events.Methods: Tissue specimens were collected from 90 patients for immunohistochemical qRT-PCR analysis miR-141 levels, respectively. Pancreatic lines used vitro assays, while nude mice vivo...
It has been demonstrated that aberrant androgen receptor (AR) signaling contributes to the pathogenesis of prostate cancer (PCa). To date, most efficacious strategy for treatment PCa remains target AR axis. However, numerous patients still face issue overtreatment or undertreatment. The establishment a precise risk prediction model is urgently needed distinguish with high-risk and select appropriate modalities.
Summary Elucidation of the pig micro RNA ome is essential for interpreting functional elements genome and understanding genetic architecture complex traits. Here, we extracted small s from skeletal muscle adipose tissue, compared their expression levels between one Western breed (Yorkshire) seven indigenous Chinese breeds. We detected 172 known porcine (mi s) 181 novel mi s. Differential analysis found 92 12 differentially expressed in tissue respectively. that different breeds shared common...
Transforming growth factor beta 1 (TGF-β1) was of importance in the pathogenesis porcine reproductive and respiratory syndrome virus (PRRSV). To determine whether knockdown TGF-β1 gene expression could facilitate control PRRSV infection, specific sequences for expressing shRNA targeted to were synthesized cloned into pSilencer 3.1-H1 neovector. Then they used transfect peripheral blood mononuclear cells Tibetan pig (Tp-PBMCs) followed by inoculation. The positive recombinant plasmids...
In order to develop cost-effective immunomodulator, the recombinant Pichia pastoris were firstly constructed co-express porcine IL-2/4/6 genes, and then fermented feed 45-days Tibetan piglets at different doses evaluate its effects on immunity of PRRS vaccination, which simultaneously received intramuscular injection inactivated vaccine. The results found that leukocytes, IgG specific antibody PRRSV, Th Tc cells increased significantly in blood treated comparison with those control (P <...
Gene expression profiles of blood can reflect the physiopathologic status immune system. The dynamic microRNA (miRNA) peripheral from pigs at different developmental stages, and how differential miRNAs might relate to system development, are unknown. In this study, samples taken five stages were used construct 15 miRNA libraries (three biological replicates/stage): 0 days (newborn), 30 (weaning), 60 (weaned), 180 360 (puberty). We identified 295 known mature miRNAs. Hierarchical clustering...
SNF5 is a key protein in regulating cell proliferation and apoptosis various cancers. However, the physiological roles of Endometrial carcinoma (EC), which one most frequent malignancies female reproduction worldwide, remains unclear. This study aims to investigate role its correlation with clinicopathologic characteristics EC. We performed immunohistochemistry detect expression 46 endometrial carcinomas 20 normal endometrium (non-EC) specimens, as well analyzed correlations between features...
IL-15 cDNA of Tibetan pig was firstly cloned from its activated lymphocytes, and then sub-cloned into VR1020 to construct recombinant VRTIL-15 plasmid study the in vitro vivo biological effects on animal. The entrapped with chitosan modified PEG-PEI (CS-PEG-PEI) transfect HEK293 cells for preliminary expression eukaryotic cells. total RNA cell isolated 48 h, successful detected by RT-PCR supernatant found stimulate significant proliferation lymphoblasts pig. Subsequently, packed CS-PEG-PEI...
Background: Estrogen-ERα signaling is an oncogenic pathway plays a crucial role in endometrial carcinogenesis. However, modulation of ERα action Endometrial cancer (ECa) remains to be elusive. Absent, small or homeotic 2-like protein (ASH2L) core component multimeric histone methyltransferase complex, which involved maintenance the active chromatin states. What function ASH2L on E2-ERα induced transactivation ECa needs further understood.Methods: Immunoprecipitation, Immunofluorescence...
Abstract Breast cancer is the most frequent malignancy in females worldwide, causing more than 680,000 deaths 2020. Dysregulated microRNAs have been linked etiologically with breast cancer, controlling a wide range of cellular pathways. Dual luciferase reporter assay was used to confirm link between miR-499a-5p and SOX6. Studies including proliferation, migration invasion were carried out investigated functional roles. Animal model introduced for vivo investigation. We found high expression...