A5021841659- Gut microbiota and health
- Neuroendocrine regulation and behavior
- Autism Spectrum Disorder Research
- Child Nutrition and Feeding Issues
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
- Diet and metabolism studies
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Parkinson's Disease Mechanisms and Treatments
- Dialysis and Renal Disease Management
- Maternal Mental Health During Pregnancy and Postpartum
- Pancreatitis Pathology and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological diseases and metabolism
- Bacterial Identification and Susceptibility Testing
- Drug Transport and Resistance Mechanisms
- Advanced Neuroimaging Techniques and Applications
- Cerebrospinal fluid and hydrocephalus
- Immune responses and vaccinations
California Institute of Technology
2018-2024
Indiana University – Purdue University Indianapolis
2022-2024
Indiana University School of Medicine
2022-2024
Indiana University
2023
The University of Texas at Austin
2012-2015
Amyloids are a class of protein with unique self-aggregation properties, and their aberrant accumulation can lead to cellular dysfunctions associated neurodegenerative diseases. While genetic environmental factors influence amyloid formation, molecular triggers and/or facilitators not well defined. Growing evidence suggests that non-identical proteins may accelerate reciprocal aggregation in prion-like fashion. humans encode ~30 amyloidogenic proteins, the gut microbiome also produces...
Despite its highly inflammatory nature, LPS is a molecule with remarkable therapeutic potential. Lipid A glycolipid that serves as the hydrophobic anchor of and constitutes potent ligand Toll-like receptor (TLR)4/myeloid differentiation factor 2 innate immune system. less toxic mixture monophosphorylated lipid species (MPL) recently became first new Food Drug Administration-approved adjuvant in over 70 y. Whereas wild-type Escherichia coli provokes strong MyD88 (myeloid primary response gene...
Autism spectrum disorder (ASD) is a neurodevelopmental condition with hallmark behavioral manifestations including impaired social communication and restricted repetitive behavior. In addition, many affected individuals display metabolic imbalances, immune dysregulation, gastrointestinal dysfunction, altered gut microbiome compositions.We sought to better understand nonbehavioral features of ASD by determining molecular signatures in peripheral tissues through mass spectrometry methods...
Autism spectrum disorder (ASD) is a neurodevelopmental characterized by heterogeneous cognitive, behavioral and communication impairments. Disruption of the gut-brain axis (GBA) has been implicated in ASD although with limited reproducibility across studies. In this study, we developed Bayesian differential ranking algorithm to identify ASD-associated molecular taxa profiles 10 cross-sectional microbiome datasets 15 other datasets, including dietary patterns, metabolomics, cytokine human...
Here we implement ultraviolet photodissociation (UVPD) in an online liquid chromatographic tandem mass spectrometry (MS/MS) strategy to support analysis of complex mixtures lipid A combinatorially modified during development vaccine adjuvants. UVPD at 193 nm was utilized characterize the structures and fragment ion types from Escherichia coli, Vibrio cholerae, Pseudomonas aeruginosa using Orbitrap spectrometer. The ions generated by were compared those collision induced dissociation (CID)...
ABSTRACT The adaptation of Pseudomonas aeruginosa to its environment, including the host, is tightly controlled by network regulatory systems. two-component system PhoPQ has been shown play a role in virulence and polymyxin resistance P. as well several other Gram-negative species. Dysregulation this demonstrated clinical isolates, yet how it affects unknown. To investigate this, an assay was used whereby bacteria were cocultured with human bronchial epithelial cells. interaction wild-type...
ABSTRACT The Vps/VacJ ABC transporter system is proposed to function in maintaining the lipid asymmetry of outer membrane. Mutations vps or vacJ Shigella flexneri resulted increased sensitivity lysis by detergent sodium dodecyl sulfate (SDS), and vpsC mutant showed minor differences its phospholipid profile compared wild type. mutants were unable form plaques cultured epithelial cells, but this was not due a failure invade, replicate intracellularly, polymerize actin via IcsA for movement...
Gram-negative bacteria have an outer membrane containing LPS. LPS is constituted of oligosaccharide portion and a lipid-A moiety that embeds this molecule within the membrane. pathogen-associated molecular pattern, several pathogens modify their as stealth strategy to avoid recognition by innate immune system gain resistance host factors disrupt bacterial cell envelope. An essential feature Salmonella enterica Typhimurium pathogenesis its ability replicate vacuoles in professional...
How do bacteria regulate their cellular physiology in response to starvation? Here, we present a detailed characterization of Escherichia coli growth and starvation over time-course lasting two weeks. We have measured multiple components, including RNA proteins at deep genomic coverage, as well lipid modifications flux through central metabolism. Our study focuses on the physiological E. stationary phase result being starved for glucose, not genetic adaptation utilize alternative nutrients....
Ultraviolet photodissociation mass spectrometry provides an unprecedented level of structural information about intact lipooligosaccharides.
ABSTRACT p -Cresol sulfate ( CS) and indoxyl (IS), gut microbiome-derived metabolites, are traditionally associated with cardiovascular disease (CVD) risks in the setting of impaired kidney function. While pharmacologic provision CS or IS can promote pro-thrombotic phenotypes, neither microbial enzymes involved nor direct production have been linked to CVD. Untargeted metabolomics was performed on a discovery cohort n = 1,149) relatively preserved function, followed by stable...
Abstract Autism is a highly heritable neurodevelopmental disorder characterized by heterogeneous cognitive, behavioral and communication impairments. Disruption of the gut-brain axis (GBA) has been implicated in autism, with dozens cross-sectional microbiome other omic studies revealing autism-specific profiles along GBA albeit little agreement composition or magnitude. To explore functional architecture we developed an age sex-matched Bayesian differential ranking algorithm that identified...
ABSTRACT Autism Spectrum Disorder (ASD) is a neurodevelopmental condition with hallmark behavioral manifestations including impaired social communication and restricted repetitive behavior. In addition, many affected individuals display metabolic imbalances, immune dysregulation, gastrointestinal (GI) dysfunction, altered gut microbiome compositions. We sought to better understand non-behavioral features of ASD by determining molecular signatures in peripheral tissues. Herein, we present the...
: Preclinical studies reveal that the microbiome broadly affects immune responses and deposition and/or clearance of amyloid-beta (Aβ) in mouse models Alzheimer's disease (AD). Whether shapes central peripheral profiles AD remains unknown.
A microbial metabolite influences myelination in the brain.