A5081244824- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- RNA modifications and cancer
- Cellular transport and secretion
- RNA Research and Splicing
- Plant Reproductive Biology
- Crop Yield and Soil Fertility
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Iron Metabolism and Disorders
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Gastric Cancer Management and Outcomes
- Esophageal Cancer Research and Treatment
- Wnt/β-catenin signaling in development and cancer
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Light effects on plants
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Plant responses to water stress
- Phytoplasmas and Hemiptera pathogens
- Phytase and its Applications
Institute of Crop Sciences
2016-2024
Chinese Academy of Agricultural Sciences
2016-2024
Shaanxi Provincial People's Hospital
2016-2024
Shanxi Medical University
2016-2024
China Agricultural University
2019-2021
Institute of Crop Science
2018
Second Affiliated Hospital of Xi'an Jiaotong University
2015
Second Peoples Hospital of Shaanxi Province
2013
The regulation of adaptive responses to phosphorus (P) deficiency by the microRNA399 (miR399)/PHOSPHATE2 (PHO2) pathway has been well studied in Arabidopsis (Arabidopsis thaliana) but not maize (Zea mays). Here, we show that miR399 transcripts are strongly induced phosphate (Pi) deficiency. Transgenic plants overexpressed MIR399b accumulated excessive amounts P their shoots and displayed typical Pi-toxicity phenotypes. We reannotated ZmPHO2 with an additional 1,165 bp 5′ untranslated region....
Summary Recent studies on nitrate transporters ( NRT s) have greatly increased our knowledge of the mechanisms regulating nitrogen (N) homeostasis in plants. However, an understanding how these s are regulated is still lacking. The limitation adaptation nla ) mutant hypersensitive to N limitation. In mutant, 15 N‐nitrate spotted old leaves preferentially accumulated youngest leaves. Analysis leaf cross‐sections indicated that NLA expression was expressed sieve element and companion cell...
Iron (Fe) deficiency remains widespread among people in developing countries. To help solve this problem, breeders have been attempting to develop maize cultivars with high yields and Fe concentrations the kernels. We conducted a genome-wide association study identified gene, ZmNAC78 (NAM/ATAF/CUC DOMAIN TRANSCRIPTION FACTOR 78), that regulates cultivated varieties both yield their kernels by using molecular marker developed from 42-base pair insertion or deletion (indel) promoter of...
Although microRNA408 (miR408) is a highly conserved miRNA, the miR408 response to salt stress differs among plant species. Here, we show that transcripts are strongly repressed by and methyl viologen treatment in maize (Zea mays). Application of N, N1-dimethylthiourea partly relieved NaCl-induced down-regulation miR408. Transgenic overexpressing MIR408b hypersensitive stress. Overexpression enhanced rate net Na+ efflux, caused locate inter-cellular space, reduced lignin accumulation, number...
Lodging is a major problem limiting maize yield worldwide. However, the mechanisms of lodging resistance remain incompletely understood for maize. Here, we evaluated 443 accessions in field. Five lodging-resistant and five lodging-sensitive were selected further research. The leaf number, plant height, stem diameter, rind penetrometer similar between -sensitive inbred lines. average thickness sclerenchymatous hypodermis layer was thicker vascular area larger lines compared with Although...
Phosphorus (P) stress is a global problem in maize production. Although macro/microarray technologies have greatly increased our general knowledge of responses to P stress, greater understanding the diversity genotypes still needed.In this study, we first evaluated tolerance low 560 accessions under field conditions, and selected P-tolerant line CCM454 P-sensitive 31778 for further research. We then generated 24 strand-specific RNA libraries from shoots roots that had been subjected 2 8...
MicroRNAs regulate gene expression at the mRNA and translational levels. Although our previous research showed that of miR169 one its targets, NFYA5, is down- up-regulated by drought stress, respectively, current study shows both NFYA5 are induced dehydration shock. Unlike overexpression MIR169a/b, MIR169i/l did not decrease transcripts but increased protein abundance also in mir169a knock-out mutants 35S::MIR169l double mutants. When bulge #11 #16 miR169/miR169* duplex were mutated,...
Abstract Recent findings have revealed the important roles of microRNAs (miRNAs) in secondary responses to oxidative damage caused by iron (Fe) excess. However, functional importance miRNAs plant Fe deficiency remains be explored. Here, we show that expression level miR164 Arabidopsis (Arabidopsis thaliana) roots was repressed deficiency. Primary root length, lateral number, ferric reductase activity, and mRNA abundance IRON-REGULATED TRANSPORTER1 (IRT1) FERRIC REDUCTION OXIDASE2 (FRO2) were...
Although the interaction between P and Zn has long been recognized in plants, physiological molecular mechanisms underlying interactions are poorly understood. We show here that supply decreases concentration maize shoots roots. Compared to +P + (addition of both Zn), +P-Zn reduced -P-Zn increased total length 1° lateral roots (LRs). Under Zn, concentrations were lower sl1 mutant than wild-type (WT) roots, accumulation did not reduce ll1 Transcriptome profiling showed auxin signaling pathway...
Although nitrogen (N) is known to affect mineral element homeostasis in plants, the molecular mechanisms of interactions between N and other nutrients remain largely unclear. We report here that supply affects ion maize. Berberine hemisulfate staining a propidium iodide penetration assay showed luxury significantly delayed Casparian strip (CS) formation maize roots. further demonstrated N-mediated CS was independent RBOHF-activated H2O2 production. induced expression ZmmiR528 whole roots...
Summary Ribosome biogenesis is a fundamental process in eukaryotic cells. Although Urb2 protein has been implicated ribosome yeast, the domain loosely conserved between plants and function of remains unknown. Here, we isolated maize mutant, designated as urb2 , with defects kernel development vegetative growth. Positional cloning transgenic analysis revealed that encodes an domain‐containing protein. Compared wild‐type ( WT ), mutant showed decreased ratios 60S/40S 80S/40S increased...
Gastric carcinoma is one of the most common human cancers and has a poor prognosis. Receptor tyrosine kinase-like orphan receptor 2 (ROR2), which non-canonical Wnt signaling pathway, been reported to be deregulated in numerous types cancers, including gastric carcinoma. However, exact role ROR2 regulation malignant phenotypes carcinoma, as well underlying molecular mechanism, remains largely unclear. The present study demonstrated that was recurrently downregulated tissues, compared with...
Approximately one-third of the global population is suffering from anaemia, with half cases being iron-deficiency anaemia (IDA). IDA has become a public health problem that particularly affects children, pregnant women and people in developing countries.1, 2 In China, government aiming at controlling prevalence children under 5 years old below 10% by 2030.3 Although iron supplements can quickly improve nutrition human, costs are relatively high. Increasing content daily consumed crops would...
The roles of C-terminal Eps15 homology domain (EHD) proteins in clathrin-mediated endocytosis plants are poorly understood. Here, we isolated a maize (Zea mays) mutant, designated ehd1, which showed defects kernel development and vegetative growth. Positional cloning transgenic analysis revealed that ehd1 encodes an EHD protein. Internalization the endocytic tracer FM4-64 was substantially reduced mutant ZmEHD1 knockout mutants. We further demonstrated ZmAP2 σ subunit physically interact at...
Colorectal cancer (CRC) is the third most common and fourth leading cause of mortality, twin studies have shown that approximately 35% variation in susceptibility to CRC involves inherited genetic differences. We sought investigate potential associations between some single nucleotide polymorphisms (SNPs) risk Chinese Han population.We conducted a case-control study including 269 cases 309 controls. Sixteen SNPs associated with were selected from previous genome-wide association genotyped...
Although previous researches have greatly increased our general knowledge on plant responses to nitrogen (N) stress, a comprehensive understanding of the different in crop genotypes is still needed. This study evaluated 304 maize accessions for low-N tolerance under field conditions, and selected sensitive Ye478 tolerant Qi319 further investigations. After 5-day treatment, typical N-deficient phenotype with yellowing older leaves was observed but not Qi319. 16 RNA libraries from leaf root...
Ribosome biogenesis is a fundamental process in all eukaryotic cells and coupled with the processing maturation of pre-rRNAs. Maize primary staple crop across world, but little known about exact pre-rRNA sites pathways this species. In study, we present detailed model pathway by identifying critical endonucleolytic cleavage determining intermediates circular reverse-transcription PCR northern blot analysis. We demonstrate that two coexist maize to promote 35S pre-rRNA, 27SA can proceed via...
Dual-specificity protein phosphatases (DUSP) negatively regulate members of the mitogen-activated kinase superfamily, which is associated with cellular proliferation and differentiation. A recent study suggests that DUSP10 frequently upregulated in colorectal cancer (CRC). Our aimed to assess whether contributes risk CRC. We analyzed nine tag single nucleotide polymorphisms (tSNPs) a case–control Han Chinese by χ2-test SHEsis software. found two tSNPs (rs908858, P=0.00004; rs11118838,...
The kernel serves as the storage organ and harvestable component of maize, it plays a crucial role in determining crop yield quality. Understanding molecular genetic mechanisms development is considerable importance for maize production. In this study, we obtained mutant, which designated defective 407 (dek407), through ethyl methanesulfonate mutagenesis. dek407 mutant exhibited reduced size weight, well delayed grain filling compared with those wild type. Positional cloning an allelism test...
Tubular anastomosis is commonly used in proximal gastrectomy; however, its use stage II esophagogastric conjugate cancer currently unclear. In this study, we investigated the short- and long-term clinical outcomes of Siewert II/III adenocarcinoma junction after modified gastrectomy with tubular compared total Roux-en-Y reconstruction. We collected data patients who underwent (PG-TEA) reconstruction (TG-RY) from October 2015 to 2018. The characteristics, postoperative quality life,...