A5086940073- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- interferon and immune responses
- Melanoma and MAPK Pathways
- Pancreatic function and diabetes
- Cytomegalovirus and herpesvirus research
- Biochemical and Molecular Research
- Animal Disease Management and Epidemiology
- Protein purification and stability
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Radio Frequency Integrated Circuit Design
- Wnt/β-catenin signaling in development and cancer
- Systemic Lupus Erythematosus Research
- 14-3-3 protein interactions
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Rheumatoid Arthritis Research and Therapies
- Cancer therapeutics and mechanisms
- Metabolism, Diabetes, and Cancer
- Calcium signaling and nucleotide metabolism
- Protein Structure and Dynamics
- Trypanosoma species research and implications
- Vector-Borne Animal Diseases
Sun Yat-sen University
2019-2021
NYU Langone Health
2011-2020
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2019
New York University
2008-2018
Harbin Veterinary Research Institute
2016-2017
Chinese Academy of Agricultural Sciences
2016-2017
Obihiro University of Agriculture and Veterinary Medicine
2012-2014
Nanjing Medical University
2014
Anhui Medical University
2012-2013
China Medical University
2013
Significance The KRAS oncogene is mutated more frequently in human cancer than any other. transcript alternatively spliced to give rise two products, K-Ras4A and K-Ras4B, both of which are oncogenic when mutated. We detected significant amounts each tumor cells colorectal carcinomas. found that targeted the plasma membrane by dual targeting motifs distinct from those K-Ras4B. Because interfering with association Ras proteins remains one most attractive approaches anti-Ras therapy, efforts...
Rac1 regulates a wide variety of cellular processes. The polybasic region the C terminus functions both as plasma membrane–targeting motif and nuclear localization sequence (NLS). We show that triproline N-terminal to contributes NLS, which is cryptic in sense it strongly inhibited by geranylgeranylation adjacent cysteine. Subcellular fractionation demonstrated endogenous nucleus Triton X-114 partition revealed this pool prenylated. Cell cycle–blocking agents, synchronization cells stably...
Ras guanosine triphosphatases (GTPases) regulate signaling pathways only when associated with cellular membranes through their C-terminal prenylated regions. proteins move between membrane compartments in part via diffusion-limited, fluid phase transfer the cytosol, suggesting that chaperones sequester polyisoprene lipid from aqueous environment. In this study, we analyze nature of pool endogenous found cytosol. The majority consists farnesylated, but not palmitoylated, N-Ras is a high...
The P2X7 receptor is a ligand-gated cationic channel that actived by ATP and normally expressed variety of immune system cells, including macrophages lymphocytes. Because it leads to release IL-1β cell death apoptosis or necrosis, potential therapeutic target for autoimmune inflammatory diseases, such as systemic lupus erythematosus (SLE). P2X7R gene highly polymorphic, many single-nucleotide polymorphisms (SNPs) have been detected. A case–control study was performed investigate the...
The mitogen-activated protein kinase kinase/extracellular regulated (MEK1/2/ERK1/2) cascade is involved in the replication of several members Flaviviridae family, including hepatitis C virus and dengue virus. effects on classical swine fever (CSFV), a fatal pestivirus pigs, remain unknown. In this study, MEK2 was identified as novel binding partner E2 CSFV using yeast two-hybrid screening. E2-MEK2 interaction confirmed by glutathione S-transferase pulldown, coimmunoprecipitation, laser...
In an attempt to improve the antitumor activity and reduce side effects of irinotecan (2), novel prodrugs SN-38 (3) were prepared by conjugating amino acids or dipeptides 10-hydroxyl group via a carbamate linkage. The synthesized compounds completely generated in pH 7.4 buffer human plasma, while remaining stable under acidic conditions. All prodrug demonstrated much greater vitro activities against HeLa cells SGC-7901 than irinotecan. most active compounds, 5h, 7c, 7d, 7f, exhibited IC50...
Cultured cells are useful for studying the subcellular distribution of proteins, including peripheral membrane proteins. Genetically encoded fluorescently tagged proteins have revolutionized study protein distribution. However, it is difficult to quantify with fluorescent microscopy, especially when partially cytosolic. Moreover, often important endogenous Biochemical assays such as immunoblots remain gold standard quantification after fractionation. Although there commercial kits that aim...
The resistance of Babesia parasites to current anti-babesiosis drugs is an issue major concern. inosine 5′-monophosphate dehydrogenase (IMPDH) gibsoni has been identified and characterized as a molecular drug target in our previous studies. In the present study, inhibitory effects IMPDH inhibitors (mycophenolate mofetil, mizoribine, ribavirin, 7-nitroindole, mycophenolic acid) were evaluated vitro or vivo. inhibition assay recombinant B. activity, mycophenolate mofetil was most potent...
Inhibiting membrane association of RAS has long been considered a rational approach to anticancer therapy, which led the development farnesyltransferase inhibitors (FTIs). However, FTIs proved ineffective against KRAS -driven tumors. To reveal alternative therapeutic strategies, we carried out genome-wide CRISPR-Cas9 screen designed identify genes required for KRAS4B association. We identified five enzymes in prenylation pathway and SAFB, nuclear protein with both DNA RNA binding domains....
Cultured cells are useful for studying the subcellular distribution of proteins, including peripheral membrane proteins. Genetically encoded fluorescently tagged proteins have revolutionized study protein distribution. However, it is difficult to quantify with fluorescent microscopy, especially when partially cytosolic. Moreover, often important endogenous Biochemical assays such as immunoblots remain gold standard quantification after fractionation. Although there commercial kits that aim...
Ras is the best-studied member of superfamily small GTPases because its role in cancer. proteins transmit signals for proliferation, differentiation and survival. Three RAS genes encode 4 isoforms. All isoforms have long been considered membrane bound, a localization required function. Our recent study revealed that N-Ras differs from all other being largely cytosolic even following modification with prenyl lipid. Endogenous, chromatographed both high low molecular weight pools, pattern...
CD8 + T cells are major components of adaptive immunity and confer robust protective cellular immunity, which requires adequate T-cell numbers, targeted migration, efficient proliferation. Altered homeostasis impaired proliferation result in dysfunctional immune response to infection or tumorigenesis. However, intrinsic factors controlling remain largely elusive. Here, we demonstrate the prominent role Brd4 on cell response. By upregulating Myc GLUT1 expression, facilitates glucose uptake...
This paper proposes a novel RF front-end tunable impedance matching network based on 65nm CMOS process, aiming at solving the instability brought by low-cost bonding wire. The pre-simulation shows that return loss is below -10 dB from 19.8 - 32 GHz, which suitable for K and Ka bands. Its 1dB bandwidth exceeds 12 GHz insertion 0.227 center frequency of 26GHz. Meanwhile, compression point (IP <inf xmlns:mml="http://www.w3.org/1998/Math/MathML"...