Hypoxia-inducible factor (HIF) is critical in the modulation of tumour angiogenesis response to hypoxia. In present study, mechanisms underlying basic fibroblast growth (bFGF)-induced activation HIF-1 and subsequent release vascular endothelial (VEGF) a human breast cancer cell line (T47D) under normoxic conditions were explored. The data show that HIF-1α expression induced by bFGF dose- time-dependent fashion, while increased protein transactivity are due phosphorylation Akt bFGF, as...

Aims Calcified chondroid mesenchymal neoplasm (CCMN) is a recently identified category of soft tissue neoplasms defined by cartilage or cartilaginous matrix formation and FN1 gene fusions. Its rarity similarities to other tumours pose diagnostic challenges. This study aims deepen understanding CCMN, highlighting molecular pathology’s role in diagnosis reduce misdiagnosis, overdiagnosis overtreatment. Methods We conducted clinicopathological analysis five newly CCMN cases reviewed 87...

Malignancy in giant cell tumor of bone (GCTB) is a rare with relevant literature being sparse. In primary malignant GCTB, distinct areas benign GCTB are juxtaposed high-grade sarcoma, while secondary sarcoma occurs at the site previously managed GCTB. This study assesses distinguishing characteristics patients this condition, time interval for development outcome treatment, and explores factors associated oncologic outcomes. retrospective case series from prospectively collected...

Although many studies have reported the negative effects of microplastics on aquatic organisms, most research is focused individual scales. Individual highlight harm mechanisms, but understanding broader ecological necessitates evidence from multiscale perspectives, particularly those based interspecific interactions. Therefore, in this study, we investigated impacts different microplastic concentrations (0, 0.4, 2, and 10 mg/L) characteristics (physiology, behavior, grazing rate) population...

H3F3A mutations and the expression of glycine 34 to tryptophan (G34W) mutants in giant cell tumors bone (GCTBs) other were detected compare mutation types G34W-mutant protein order provide a theoretical basis for using as diagnostic differential-diagnostic tool GCTBs. A total 366 tumor cases investigated. The involved 215 men 151 women, whose median age was 29 years (3-84). included GCTB (n=180), recurrent (n=19), with lung metastasis (n=5), pediatric (n=15), primary malignant...

Abstract Background The clinical and molecular characteristics of osteofibrous dysplasia (OFD)-like adamantinoma (AD) differ from those classic AD. Most reports about OFD-like AD are case or small series. More cases different centers still warranted. Methods orthopedic oncology database our institution was searched to identify patients with the long bones were retrospectively analyzed. Between December 1999 August 2016, 23 treated for AD, comprising seven 16 outcomes compared between...

Dedifferentiated central chondrosarcoma (DCCS) is a rare cartilage tumor with invasive biological behavior and poor prognosis. To better understand the morphological characteristics of this type its internal mechanism dedifferentiation, we retrospectively analyzed 57 cases DCCS. A total 29 female 28 male patients were included, ranging in age from 20 to 76 years, median 54 years. Fifty-seven DCCS occurred pelvis ( n = 29), femur 17), scapula 4), tibia 2), humerus metatarsals 1), fibula...

Osteoid osteoma is type of benign bone tumor, characterized by a well-demarcated core with typical size <1 cm and distinctive surrounding zone reactive formation. The tumor can occur anywhere in the cortex or medulla skeleton. However, lesion usually affects long bones lower extremities. present study describes two cases osteoid osteomas located rib.

Abstract Background Recently, a novel group of CD34 and S100 co-expression spindle cell tumors with distinctive stromal perivascular hyalinization harboring recurrent gene fusions involving RET , RAF1 BRAF NTRK1/2 has been identified. Case presentation In this study, we reported two Chinese male patients soft tissue presenting in the right knee joint left thigh, respectively. For both patients, were completely excised clear margin. Microscopically, case 1showed morphological overlap...

Objective: To investigate the clinicopathological characteristics, histogenesis, immunophenotypes and molecular genetic features of primary intraosseous Rosai-Dorfman disease (RDD) for improving diagnostic accuracy differential diagnosis. Methods: This retrospective study included 14 RDD cases diagnosed from January 2009 to 2019 at Beijing Jishuitan Hospital, China. The immunohistochemical staining S-100, cyclin D1, CD1a CD207 expression was analyzed. BRAF V600E KRAS mutation analyses were...

Objective: To study the clinicopathologic features of dedifferentiated liposarcoma extremities. Methods: Nine cases extremities diagnosed at Beijing Jishuitan Hospital from 2009 to 2017 were selected. The histological evaluated by HE and immunohistochemistry, together with clinical radiological features. Flourescence in situ hybridization(FISH) was used detect MDM2 amplification. Results: They located thigh (6 cases), calf (2 cases) buttock (1 case). There six females three males. Patients'...

To the Editor: In recent years, total joint replacement has become most effective method to preserve function. However, with increase in numbers of surgeries, attention post-operative complications increased dramatically. The main complication is loosening prosthesis. Loosening can be caused by periprosthetic infection (PJI).[1] histopathology tissue one commonly used methods for diagnosing PJI. Since neutrophils reflect body's response infection, diagnosis PJI determined neutrophil counts...

透明细胞肉瘤（clear cell sarcoma，CCS）是一种少见的恶性间叶性肿瘤，占所有软组织肉瘤的1%，常发生于足踝关节深部，与肌腱和腱膜相关，原发于骨内的CCS极其罕见。由于CCS组织形态多样，且与黑色素瘤具有相似的组织学和免疫组织化学特性，因此极易与黑色素瘤混淆。本文报道1例误诊为黑色素瘤的骨内原发性CCS，介绍其临床表现、影像学检查、组织学特征、细胞遗传学及其鉴别诊断要点。.

Hydrogen sulfide (H₂S) is a novel gas transmitter signaling molecule. H₂S synthesized by cystathionine β-synthase (CBS), γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST). There have been no reports about the roles of these enzymes in osteosarcoma its metastases. We detected synthase expression levels human primary lung metastatic osteosarcoma.Immunohistochemistry was performed (n=19), (n=11), osteoblastoma (n=10) bony callus (n=2). The CBS, CSE, MST defined as negative,...

Objective: To identify the clinical outcome and prognostic factors of synchronous multicentric osteosarcoma (SMOS). Methods: The data 2 602 conventional patients admitted to Beijing Jishuitan Hospital from January 1995 June 2018 were retrospectively analyzed. Finally, 56 (2.1%) cases confirmed as SMOS according imaging database, medical record pathological results.All epidemiological cases,initial diagnosis time, tumor site, number lesions, chemotherapy, surgical treatment, alkline...

Objective: To investigate the radiological and histopathological features of giant cell tumor bone treated with RANKL inhibitor denosumab. Methods: Eleven cases were retrieved from surgical pathology records between March 2015 June 2017 in Beijing Jishuitan Hospital. Formalin fixed, paraffin embedded specimens collected histological evaluated. The imaging including X ray, magnetic resonance imaging, computed tomography also reviewed. Results: These 11 derived five female six male patients,...

Objective: To investigate the subtypes of H3F3A DNA mutation in H3.3 immunohistochemistry (IHC) negative giant cell tumors bone (GCTB). Methods: IHC expression G34W mutated protein was evaluated 181 cases GCTB. In cases, Sanger sequencing analysis used to detect mutations. Results: Overall, 164 (90.61%) GCTB showed nuclear H3.3, and 17 were negative. These subjected analysis; results that eight presented rare occurring at glycine 34 leucine (G34L, 3/181, 1.66%), valine (G34V, 1.66%) arginine...