A5102890630- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Liver physiology and pathology
- Transplantation: Methods and Outcomes
- T-cell and B-cell Immunology
- Pulmonary Hypertension Research and Treatments
- RNA Research and Splicing
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Mechanical Circulatory Support Devices
- RNA and protein synthesis mechanisms
- Meat and Animal Product Quality
- Clinical Nutrition and Gastroenterology
- Drug Transport and Resistance Mechanisms
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Phosphodiesterase function and regulation
- Complement system in diseases
Betta Pharmaceuticals (China)
2022-2023
Kyoto University
2010-2023
North China University of Technology
2022
Hebei Agricultural University
2018-2022
Henan Provincial Chest Hospital
2022
Augusta University Health
2019
Dalian University of Technology
2019
Tongji University
2017
Southwest Medical University
2017
Affiliated Hospital of Southwest Medical University
2017
Integrin alpha v beta 3 is distinct in its capacity to recognize the sequence Arg-Gly-Asp (RGD) many extra-cellular matrix (ECM) components. Here, we demonstrate that addition recognition of ECM components, can interact with neural cell adhesion molecule L1-CAM; a member immunoglobulin superfamily (IgSF). M21 melanoma cells displayed significant Ca(++)-dependent and spreading on immunopurified rat L1 (NILE). This was found be dependent expression v-integrin subunit could significantly...
Antibody-mediated rejection (AMR) remains a refractory after donor-specific antibody (DSA)-positive or blood-type incompatible liver transplantation (LT), even in the era of pre-transplant rituximab desensitization. This is due to lack not only effective post-transplant treatments but also robust animal models develop/validate new interventions. Orthotopic LT from male Dark Agouti (DA) Lewis (LEW) rats was used develop rat LT-AMR model. LEW were pre-sensitized by preceding skin DA 4–6 weeks...
Some experimental transplant-tolerance models have shown that the presence of regulatory T cells within grafts is important for development tolerance (Tol). METHODS.: To determine if correlates with graft acceptance in living-donor liver-transplantation tolerance, expression Foxp3 mRNA and CD4, CD8, were quantified biopsies from tolerant recipients by real-time polymerase chain reaction immunohistochemistry immunofluorescent staining (Gr-Tol). The results compared on maintenance...
Background. Operational tolerance is defined as long-term acceptance of a transplanted organ after complete cessation immunosuppression (IS), but may not always protect against antigen-dependent changes in graft morphology. Method. IS free patients living-donor liver transplantation (LDLT) underwent protocol biopsy (tolerance group [Gr-Tol]) and were evaluated for rejection fibrosis. The degree fibrosis was compared with those the on maintenance (Gr-IS) base line normal (Gr-BS). When...
Intestinal transplantation (ITx) remains challenged by frequent/severe rejections and immunosuppression-related complications (infections/malignancies/drug toxicity). We developed the Leuven Immunomodulatory Protocol (LIP) in lab translated it to clinics. LIP consists of experimentally proven maneuvers, destined promote T-regulatory (Tregs)-dependent graft-protective mechanisms: donor-specific blood transfusion (DSBT); avoiding high-dose steroids/calcineurin-inhibitors; minimizing...
To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.We describe surgical procedures rat OLT model, determined operator curves. The various factors that contributed to surgery were determined. evaluated between unsuccessful surgeries.Learning curve data indicated 50 cases required training start a study. Operative time, blood loss, warm ischemic anhepatic phase, unstable systemic hemodynamic state, body...
T-cell receptor Vδ2 γδ T cells (Vδ2 cells) participate in host defense, whereas Vδ1 (Vδ1 may regulate immune responses. appear to play a role fetomaternal tolerance and our aim was examine their liver transplant tolerance.To determine whether increase within accepted grafts after semiallogeneic pediatric transplantation, the Vδ1/Vδ2 ratio assessed at transcriptional level complementarity-determining region 3 loop of δ chain sequenced biopsies from immunosuppression-free (n=6) or almost free...
Polyamines are essential for cell growth and differentiation. They play important roles in protection from liver damage promotion of regeneration. However, little is known about the effect oral exogenous polyamine administration on This study investigated impact polyamines (spermidine spermine) ischemia/reperfusion injury (IRI) We used a rat model which 70% hepatectomy after 40 minutes ischemia was performed to mimic clinical condition living donor partial transplantation (LT). Male Lewis...
In patients with cerebral infarction (CI), elevated serum uric acid (UA) level may exacerbate the occurrence and development of carotid atherosclerosis (AS). Our study intended to explore underlying mechanism. We enrolled 86 CI, divided them into four groups: Non-AS, AS-mild, AS-moderate, AS-severe groups; levels UA oxidative stress-related factors in were detected. The middle artery occlusion (MCAO) model was used stimulate CI rats, different doses administrated. Hematoxylin & eosin (H&E)...
Antibody-mediated rejection (AMR) is a refractory after ABO blood-type incompatible (ABOi) or donor-specific antibody (DSA)-positive liver transplantation (LT). Pretransplant rituximab desensitization dramatically reduced posttransplant AMR development; however, risk factors for in the era remain unclear both ABOi living-donor LT (ABOi-LDLT) and preformed DSA-positive (pDSA-LT). Of our 596 adult LDLTs (≥18 y) introduction (2004–2019), 136 were ABOi-LDLT (22.8%). After excluding retransplants...
CD4+CD25++CD45RA+ cells (naïve regulatory T [naïve-Tregs]) have been identified as a functionally premature form of CD4+CD25+++CD45RA(-) (conventional-Tregs). However, their contribution to transplant tolerance remains be elucidated.We examined the frequency and function conventional naive-Tregs in peripheral blood derived from operationally tolerant patients after pediatric living-donor liver (Gr-tol). The data were compared with those who unable weaned off immunosuppression due rejection...
Although checkpoint inhibitors (CPIs) have changed the paradigm of cancer therapy, low response rates and serious systemic adverse events remain challenging. In situ vaccine (ISV), intratumoral injection immunomodulators that stimulate innate immunity at tumor site, allows for development vaccines in patients themselves. K3-SPG, a second-generation nanoparticulate Toll-like receptor 9 (TLR9) ligand consisting K-type CpG oligodeoxynucleotide (ODN) wrapped with SPG (schizophyllan), integrates...
Ulcerative colitis (UC) is an idiopathic inflammatory disease. We intend to explore the mechanism of Rutin in therapy UC. Disease activity index (DAI) and hematoxylin-eosin staining were employed assess therapeutic effect on dextran sulfate sodium-stimulated mice. The proliferation was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. Oxidative stress (OS) assessed measuring reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD)....
Abstract The Hippo signaling pathway is critical for the regulation of organ development, tissue homeostasis, and tumorigenesis by controlling activation status yes-associated protein (YAP) or its homolog PDZ-binding motif (TAZ). As a major downstream effector, transcription factor TEAD activated forming complex with YAP/TAZ. aberrations, such as NF2-deficiency LATS1/2 mutations leading to hyperactivation YAP/TAZ subsequent TEAD, have been reported in many cancers, including mesothelioma,...
Background & AimsAcute liver failure (ALF) is a life-threatening condition with limited treatment alternatives. ALF pathogenesis seemingly involves the complement system. However, no complement-targeted intervention has been clinically applied. In this study, we aimed to investigate potential of Complement-5 (C5)-targeted treatment.MethodsALF was induced in C5-knockout (KO, B10D2/oSn) mice and their wild-type (WT) counterparts (B10D2/nSn) through intraperitoneal lipopolysaccharide (LPS)...
The outcomes of liver transplantation (LT) from donation after cardiac death (DCD) donors remain poor due to severe warm ischemia injury. Perfluorocarbon (PFC) is a novel compound with high oxygen carrying capacity. In the present study, rat model simulating DCD LT was used, and impact improved graft oxygenation provided by PFC addition on ischemia/reperfusion injury (IRI) survival investigated. Orthotopic transplants were performed in male Lewis rats, using grafts preserved cold University...
Donor-recipient human leukocyte antigen (HLA) compatibility has not been considered to significantly affect liver transplantation (LT) outcomes; however, its significance in living-donor LT (LDLT), which is mostly performed between blood relatives, remains unclear. This retrospective cohort study included 1954 LDLTs at our institution (1990-2020). The primary and secondary endpoints were recipient survival the incidence of T cell-mediated rejection (TCMR) after LDLT, respectively, according...