Rotator cuff (RC) attaches to humerus across a triphasic yet continuous tissue zones (bone-fibrocartilage-tendon), termed "enthesis". Regrettably, rapid and functional enthesis regeneration is challenging after RC tear. The existing grafts bioengineered for repair are insufficient, as they were engineered by scaffold that did not mimic normal in morphology, composition, tensile property, meanwhile cannot simultaneously stimulate the formation of bone-fibrocartilage-tendon tissues. Herein, an...

Cardiac fibroblasts (CFs) are the primary cell type responsible for cardiac fibrosis during pathological myocardial remodeling. Several studies have illustrated that pirfenidone (5-methyl-1-phenyl-2-[1H]-pyridone) attenuates in different animal models. However, effects of on fibroblast behavior not been examined. In this study, we investigated whether directly modulates is important remodeling such as proliferation, myofibroblast differentiation, migration and cytokine secretion. Fibroblasts...

Background: A repaired rotator cuff (RC) often heals with interposed scar tissue, making repairs prone to failure. Urine-derived stem cells (USCs), robust proliferation ability and multilineage differentiation, can be isolated from urine, avoiding invasive painful surgical procedures for harvesting the cells. These advantages make it a novel cell source autologous transplantation enhance RC healing. Hypothesis: Implantation of an autogenous USC sheet injury site will Study Design: Controlled...

Anterior cruciate ligament (ACL) rupture is a common sports injury, which causes knee instability and abnormal joint kinematics. The current ACL graft was single-phasic, not convenient for the formation of enthesis-like tissue in bone tunnel, resulting poor integration graft-to-bone. A band-shaped acellular tendon (BAT) prepared as core component reconstruction at first, while sleeve-shaped cartilage (SAC) or (SAB) fabricated using vacuum aspiration system (VAS)-based decellularization...

Abstract Background This present study is aimed to retrospectively evaluate the efficacy and safety of a novel personalized navigation template in proximal femoral corrective osteotomy for treatment DDH. Methods Twenty-nine consecutive patients with DDH who underwent were evaluated between August 2013 June 2017. Based on different surgical methods, they divided into conventional group ( n = 14) 15). The degrees, radiation exposure, operation time compared two groups. Results No major...

Achilles tendon (AT) defects often occur in traumatic and chronic injuries. Currently, no graft can satisfactorily regenerate parallel tendinous tissue at the defect site to completely restore AT function.To develop a cell-free functional by tethering bone morphogenetic protein 12 (BMP-12) on book-shaped decellularized matrix (BDTM) determine whether this is more beneficial for healing than an autograft.Controlled laboratory study.Canine patellar was sectioned into book shape fabricate BDTM....

Functional and rapid enthesis regeneration remains a challenge after arthroscopic rotator cuff (RC) repair. Tissue-engineering large-size biomimetic scaffold may be an adjuvant strategy to improve this clinical dilemma. Herein, we developed optimized protocol decellularize as scaffolds for augmenting RC tear.A novel vacuum aspiration system (VAS) was set up, which can provide negative pressure suck out cellular substances from tissue blocks without using chemical detergents. Large-size...

Rapid and functional bone-tendon (B-T) healing remains a difficulty in clinical practice. Tissue engineering has emerged as promising strategy to address this problem. However, the majority of tissue scaffolds are loaded with stem cells enhance regenerability B-T healing, which is complicated inconvenient for application. Accordingly, developing cell-free scaffold chemotactic function chondrogenic inducibility may be an effective approach. In study, collagen affinity peptide derived from A3...

Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising to humans exists significant hurdle. For solving this problem, canine has been developed as new large animal model bridge the gap from rodents humans. In study, we reported isolation and characterization urine-derived stem cells (USCs) mature healthy beagle dogs. The isolated showed fibroblast-like morphology had good clonogenicity...

Rotator cuff tear (RCT) is among the most common shoulder injuries and prone to rerupture after surgery. Selecting suitable subpopulations of stem cells as a new specific cell type mesenchymal has been increasingly used potential therapeutic tool in regenerative medicine. In this study, murine adipose-derived SSEA-4+CD90+PDGFRA+ subpopulation were successfully sorted, extracted, identified. These showed good proliferation differentiation potential, especially direction tendon...

Abstract Background Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide natural three-dimensional scaffold with tissue-specific orientations of molecules for regeneration, however, the distributions collagen PGs content book-shaped scaffolds rabbit rotator cuff by SR-FTIR have not been reported. Methods Native tissues...

Tendon-bone interface (TBI) healing is a clinical dilemma that closely relevant to new bone formation and remodeling at the repair site. Previous studies showed metformin an osteogenic inducer for stem cells in vitro capable of stimulating regeneration vivo.Metformin would be effective promoting TBI by enhancing remodeling.Controlled laboratory study.Canine marrow (BMSCs) were cultured with various concentrations (0, 10, 50, 100, 200 μM). The effect on differentiation canine BMSCs was...

Atrophic nonunion is one of the most difficult complications fracture. The cellular factors that contribute to atrophic are poorly understood, and mesenchymal stem cells (MSCs) recognized as key contributor bone formation. This study aimed characterize MSCs isolated from fibrotic tissue (AN-MSCs) perspective proliferation, differentiation potential, senescence, paracrine function.Human was obtained four donors aged 29-37 for isolating AN-MSCs, donor-matched marrow acquired iliac crest...

Abstract Purpose To assess the preoperative planning of visualized simulative surgery (VSS) and clinical outcomes based on computer-aided design (CAD) 3D reconstruction for proximal femoral varus osteotomy DDH. Methods A total 31 consecutive patients (23 females 8 males) with DDH who underwent were retrospectively reviewed between June 2014 July 2018. Patients divided into conventional group ( n = 15) VSS 16) according to different surgical methods. In group, 16 evaluated preoperatively aid...

This study aimed to assess the clinical and radiologic outcomes of a functional brace in combination with physical therapy (FBPT) for early correction cubitus varus young children.Eighteen consecutive patients secondary supracondylar fractures were enrolled between July 2017 March 2019. We used FBPT technique correct sagittal plane deformity children. The evaluation included measurement angulation, plane, range motion at three, six, twelve months post-intervention. radiographic results...

The aim of this study is to retrospectively evaluate the efficacy 3D navigational template for Salter osteotomy DDH in children.Thirty-two consecutive patients with who underwent were evaluated between July 2014 and August 2017, they divided into conventional group (n = 16) navigation according different surgical methods. corrective acetabular degrees, radiation exposure, operation time compared two groups.No nerve palsy or redislocation was reported group. Compared group, had advantages...

<title>Abstract</title> <bold>Background</bold>: 8-plate hemiepiphysiodesis is widely used for treatment of genu varum. This present study aimed to retrospectively explore the feasibility and efficacy a novel computer-assisted drill guide template guided correction varum in children. <bold>Methods</bold>: Twenty-eight patients who underwent due from June 2014 2018 were analyzed our hospital. All cases treated with ultra-low-dose computed tomography (CT) scans knee joint before surgery. Novel...

Background The aim of this study was to assess the clinical and radiographic outcomes cubitus varus treatments based on different fixation methods: Locking plate vs. Kirschner-wires (K-wires) cast fixation. Methods This retrospective 28 patients performed in lateral-wedge osteotomy for deformity our hospital from July 2018 2020. 14 group A were treated by locking after lateral closing-wedge osteotomy, whereas other K-wires B. We measured bony union carrying angle. assessed according...

This study was aimed to assess the feasibility and efficacy of 3D printing digital template for treatment cubitus varus deformity.