A5030832172- Radiopharmaceutical Chemistry and Applications
- Prostate Cancer Treatment and Research
- Urinary Bladder and Prostate Research
- RNA modifications and cancer
- Prostate Cancer Diagnosis and Treatment
- Hormonal and reproductive studies
- Peptidase Inhibition and Analysis
- Antifungal resistance and susceptibility
- Lung Cancer Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Antibiotic Resistance in Bacteria
- Fungal Infections and Studies
- Urological Disorders and Treatments
- Cancer-related molecular mechanisms research
- Diet and metabolism studies
- Genetics, Aging, and Longevity in Model Organisms
- Muscle metabolism and nutrition
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Estrogen and related hormone effects
- Alcohol Consumption and Health Effects
- Pelvic floor disorders treatments
University of Wisconsin–Madison
2018-2024
The University of Texas Southwestern Medical Center
2021
University of Pittsburgh
2014-2016
University at Albany, State University of New York
2012
Albany State University
2012
Le Bonheur Children's Hospital
2005-2010
University of Tennessee at Knoxville
2010
University of Tennessee Health Science Center
2005-2009
Children's Medical Center
2005-2009
Molecular Sciences Institute
2008
Constitutive overexpression of the MDR1 (multidrug resistance) gene, which encodes a multidrug efflux pump major facilitator superfamily, is frequent cause resistance to fluconazole and other toxic compounds in clinical Candida albicans strains, but mechanism upregulation has not been resolved. By genome-wide gene expression analysis we have identified zinc cluster transcription factor, designated as MRR1 regulator), that was coordinately upregulated with drug-resistant, C. isolates....
Antifungal agents exert their activity through a variety of mechanisms, some which are poorly understood. We examined changes in the gene expression profile Candida albicans following exposure to representatives four currently available classes antifungal used treatment systemic fungal infections. Ketoconazole increased genes involved lipid, fatty acid, and sterol metabolism, including NCP1, MCR1, CYB5, ERG2, ERG3, ERG10, ERG25, ERG251, that encoding azole target, ERG11. also several...
In the pathogenic yeast Candida albicans, zinc cluster transcription factor Upc2p has been shown to regulate expression of ERG11 and other genes involved in ergosterol biosynthesis upon exposure azole antifungals. encodes lanosterol demethylase, target enzyme this antifungal class. Overexpression UPC2 reduces susceptibility, whereas its disruption results hypersusceptibility azoles reduced accumulation exogenous sterols. leads increased production which contributes resistance clinical...
We present a comprehensive map of over 1 million polyadenylation sites and quantify their usage in major cancers tumor cell lines using direct RNA sequencing. built the Expression Polyadenylation Database to enable visualization maps various facilitate discovery novel genes gene isoforms that are potentially important tumorigenesis. Analyses indicate large fraction (∼30%) mRNAs contain alternative 3' untranslated regions, independent type. The shortest region preferentially upregulated...
A major mechanism of azole resistance in Candida albicans is overexpression the genes encoding ATP binding cassette transporters Cdr1p and Cdr2p due to gain-of-function mutations Tac1p, a transcription factor zinc cluster family. To identify Tac1p regulon, we analyzed four matched sets clinical isolates representing development CDR1- CDR2-mediated by using gene expression profiling. We identified 31 that were consistently up-regulated with CDR1 CDR2, including TAC1 itself, 12 down-regulated...
ABSTRACT Cap1p, a transcription factor of the basic region leucine zipper family, regulates oxidative stress response (OSR) in Candida albicans . Alteration its C-terminal cysteine-rich domain (CRD) results Cap1p nuclear retention and transcriptional activation. To better understand function C. , we used genome-wide location profiling (chromatin immunoprecipitation-on-chip) to identify targets vivo. A triple-hemagglutinin (HA 3 ) epitope was introduced at C terminus wild-type (Cap1p-HA or...
ObjectivesThe aim of this study was to identify changes in the gene expression profile Streptococcus pneumoniae response a subinhibitory concentration penicillin an effort better understand mechanisms by which organism copes with stress.
Prostate disease incidence, both benign and malignant, directly correlates with age. Men under 40 years of age are rarely diagnosed or malignant prostate disease, while 90% men over the 80 have histological evidence (benign prostatic hyperplasia; BPH). Although rodent models been invaluable in study progression treatment efficacy, effect is often not considered. In examining aged (24-month-old) mice, we observed changes within lower urinary tract that typically associated dysfunction (LUTD)...
The aim of this study was to identify changes in the gene expression profile Candida albicans upon exposure hydroxypyridone anti-infective agent ciclopirox olamine an effort better understand its mechanism action.C. SC5314 exposed either medium alone or at a concentration equivalent IC50 (0.24 mg/L) for 3 h. RNA isolated and profiles were compared using DNA microarrays. Differential select genes confirmed by real-time reverse transcription (RT)-PCR. Mutants disrupted CDR2 both CDR1 CDR2, as...
MUC7 12-mer is a cationic antimicrobial peptide derived from the N-terminal region of human low-molecular-weight salivary mucin. In order to gain new insights into modes action against opportunistic fungal pathogen Candida albicans, we examined changes in gene expression profile C. albicans upon exposure this peptide. Cells at an early logarithmic phase were exposed 6 μM and grown until OD600 nm approximately 0.4 was reached. Changes determined by microarray analysis showed that 19 out total...
Age is the greatest risk factor for lower urinary tract symptoms attributed to benign prostatic hyperplasia (LUTS/BPH). Although LUTS/BPH can be managed with pharmacotherapy, treatment failure has been putatively numerous pathological features of BPH (eg, fibrosis, inflammation). Mitochondrial dysfunction a hallmark aging; however, its impact on remains unknown. Publicly available gene array data were analyzed. Immunohistochemistry examined mitochondrial proteins in human prostate. The...
The highly conserved, multifunctional YB-1 is a powerful breast cancer prognostic indicator. We report on pervasive role for in which it associates with thousands of nonpolyadenylated short RNAs (shyRNAs) that are further processed into small (smyRNAs). Many these have previously been identified as functional noncoding (http://www.johnlab.org/YB1). novel, abundant, 3′-modified RNA antisense to Dicer1 (Shad1) colocalizes P-bodies and stress granules. expression Shad1 was shown correlate whose...
<h3>Importance</h3> Benign prostatic hyperplasia (BPH) in older men can cause lower urinary tract symptoms (LUTS), which are increasingly managed with medications. Frailty may contribute to both symptom progression and serious adverse events (SAEs), shifting the balance of benefits harms drug therapy. <h3>Objective</h3> To assess association between a deficit accumulation frailty index clinical BPH or SAE. <h3>Design, Setting, Participants</h3> This cohort study used data from Medical...
Abstract Background Older men frequently develop lower urinary tract symptoms attributed to benign prostatic hyperplasia (LUTS/BPH). Risk factors for LUTS/BPH include sedentary lifestyle, anxiety/depression, obesity, and frailty, which all increase with age. Although physical exercise may reduce the progression and/or severity of LUTS/BPH, age-related mechanisms responsible remain unknown. Methods Voiding symptoms, body mass, frailty were assessed after 4-weeks voluntary wheel running in...
Patients with prostate cancer treated androgen deprivation therapy (ADT) eventually develop castrate-resistant (CRPC). 1,25-Dihydroxyvitamin D3 (1,25D3/calcitriol) is a potential adjuvant that confers antiproliferative and pro-differentiation effects in vitro, but has had mixed results clinical trials. The impact of the tumor microenvironment on 1,25D3 patients CRPC not been assessed. Transforming growth factor β (TGFβ), which associated development tumorigenic "reactive stroma" cancer,...
Benign prostatic hyperplasia (BPH) is an age-related disease that affects millions of aging males globally. While the pathogenesis BPH remains incompletely understood, emerging evidence suggests a pivotal role for androgen receptor (AR) in mediating prostate growth and function. Understanding AR signaling alteration may inform novel treatments. Here, we analyzed protein expressions AR, NKX3.1, Ki-67 young (2 months) aged (24 mice. We also examined potential mechanism expression. Compared to...
Current pharmacotherapies for symptomatic benign prostatic hyperplasia (BPH), an androgen receptor-driven, inflammatory disorder affecting elderly men, include 5α-reductase (5AR) inhibitors (i.e. dutasteride and finasteride) to block the conversion of testosterone more potent receptor ligand dihydrotestosterone. Because dihydrotestosterone is precursor estrogen β (ERβ) ligands, 5AR could potentially limit ERβ activation, which maintains prostate tissue homeostasis. We have uncovered...
The void spot assay (VSA) is a cost-effective method for evaluating and quantifying mouse urinary voiding phenotypes. VSA has been used to differentiate behaviors between experimental groups, but not as diagnostic assay. To build toward this goal, we the define patterns of male mice with diabetic diuresis (BTBR.Cg-Lepob /WiscJ mice), irritative dysfunction (E. coli UTI89 tract infection), obstructive (testosterone estradiol slow-release implants) compared their respective controls. Many...
Abstract Low protein (LP) diets promote health and longevity in diverse species. Although the precise components of an LP diet that mediate its beneficial effects have not been defined, reducing dietary levels three branched-chain amino acids (BCAAs) leucine, isoleucine valine promotes metabolic both sexes, increases lifespan while frailty male, but female, C57BL/6J mice. Each BCAA has unique effects, we recently showed restriction is sufficient to required for benefits male Here, tested...
You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (PD03)1 May 2024PD03-06 MITOCHONDRIAL COMPLEX I PROTEIN NDUFS3 IS DECREASED IN BENIGN PROSTATIC HYPERPLASIA PATIENTS TREATED WITH CELECOXIB AND/OR FINASTERIDE Chandler N. Hudson, Teresa T. Liu, Taro Igarashi, Nathalie El-Khoury, Nnamdi Ihejirika, Kelsey Paxton, Juliann Jaumotte, Rajiv Dhir, Joel B. Nelson, Donald DeFranco, William A. Ricke, Naoki Yoshimura, Zhou Wang, and Laura E. Pascal...
Abstract Background Benign prostatic hyperplasia (BPH) is a condition generally associated with advanced age in men that can be accompanied by bothersome lower urinary tract symptoms (LUTS) including intermittency, weak stream, straining, urgency, frequency, and incomplete bladder voiding. Pharmacotherapies for LUTS/BPH include alpha‐blockers, which relax urethral smooth muscle 5ɑ‐reductase inhibitors such as finasteride, block conversion of testosterone to dihydrotestosterone thereby...