A5085107380- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Retinal Development and Disorders
- Aquatic Ecosystems and Phytoplankton Dynamics
- Biocrusts and Microbial Ecology
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Pulmonary Hypertension Research and Treatments
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Cardiac Fibrosis and Remodeling
- Marine Toxins and Detection Methods
- RNA Interference and Gene Delivery
- Cardiovascular Function and Risk Factors
- Respiratory Support and Mechanisms
- Retinal Diseases and Treatments
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Magnetic properties of thin films
National Cerebral and Cardiovascular Center
2013-2025
Osaka University
1997-2024
Nara Institute of Science and Technology
2023
Hitachi (Japan)
2005-2019
Ibaraki University
1989-2011
Meijo University
2011
Lung Institute
2009
Nagoya University
2009
Imperial College London
2009
Kyushu University
2008
Journal Article Polyketide Synthase Gene Coupled to the Peptide Synthetase Module Involved in Biosynthesis of Cyclic Heptapeptide Microcystin Get access Tomoyasu Nishizawa, Nishizawa *Dwiswn Biotechnology, School Agriculture, Ibaraki UniversityAmi, 300–0393 Search for other works by this author on: Oxford Academic PubMed Google Scholar Akiko Ueda, Ueda Munehiko Asayama, Asayama Kiyonaga Fujii, Fujii †Faculty Pharmacy, Meijo UniversityTempaku, Nagoya 468–8503 Ken-ichi Harada, Harada Kozo...
ABSTRACT The rae28 gene is a mouse homologue of the Drosophila polyhomeotic (Nomura, M., Takihara, Y. and Shimada, K. (1994) Differentiation 57, 39-50), which member Polycomb group (Pc-G) genes (DeCamillis, Cheng, N., Pierre, D. Brock, H.W. (1992) Genes Dev. 6, 223-232). Pc-G are required for correct expression Homeotic complex segment specification during embryogenesis larval development. To study role in development, we generated rae28-deficient mice by targeting embryonic stem cells....
Abstract The origin and developmental mechanisms underlying coronary vessels are not fully elucidated. Here we show that myocardium-derived angiopoietin-1 (Ang1) is essential for vein formation in the developing heart. Cardiomyocyte-specific Ang1 deletion results defective of subepicardial veins, but had no significant effect on intramyocardial arteries. endothelial cells (ECs) sinus venosus (SV) heterogeneous population, composed APJ-positive APJ-negative ECs. Among these, ECs migrate from...
Significance Inflammatory signals are thought to be crucial for the pathogenesis of PAH; however, underlying mechanism is still largely unknown. In this study, we demonstrate that AHR makes a causal contribution PAH, activating focal inflammatory response in lungs and promoting infiltration immune cells from bone marrow. Furthermore, found PAH patients with higher agonistic activity sera more susceptible severe clinical events than those lower activity. Because conventional therapy pulmonary...
Pulmonary arterial hypertension (PAH) is characterized by stenosis and occlusions of small pulmonary arteries, leading to elevated pressure right heart failure. Although accumulating evidence shows the importance interleukin (IL)-6 in pathogenesis PAH, target cells IL-6 are poorly understood. Using mice harboring floxed allele gp130 , a subunit receptor, we found substantial Cre recombination all hematopoietic cell lineages from primitive stem level SM22α-Cre mice. We also revealed that CD4...
During early heart development, Tbx2 gene expression is initiated in the cardiac crescent and then becomes restricted to outflow tract atrioventricular region. We identified a regulatory region, enriched multiple Smad sites, sufficient reproduce patterns overlapping Bmp2 Bmp4 activity heart. The role of cardiogenesis was analyzed by using Cre-LoxP activated transgenic misexpression chamber myocardium. Ventricular exhibited an abnormally narrow lumen owing expansion Hyaluronan synthase 2 ECM...
<title>Abstract</title> The plasticity of blood mononuclear cells (MCs) and their role in vascular remodeling have been the focus many studies; however, <italic>in vitro</italic> differentiation efficiency remains poorly understood. Herein, we demonstrate that inflammatory response accelerates MCs into endothelial-like through chemical cues vitro</italic>. RT-PCR RNA sequencing revealed differentiated exhibited upregulated pathways associated with regeneration. In contrast, collected from...
All samples of cyanobacterial blooms collected from 1986 to 1989 Lake Kasumigaura, Ibaraki Prefecture, Japan, were hepatotoxic. The 50% lethal doses (LD50s) the mice ranged 76 556 mg/kg body weight. Sixty-eight Microcystis cell clones (67 aeruginosa and 1 M. viridis) isolated blooms. Twenty-three strains (including viridis strain) toxic. However, ratio toxic nontoxic among varied (6 86%). Microcystins examined in six strains. Five produced microcystin-RR, -YR, -LR, with RR being dominant...
Abstract Subarachnoid hemorrhage due to rupture of an intracranial aneurysm has a quite poor prognosis after the onset symptoms, despite modern technical advances. Thus, mechanisms underlying lesions should be clarified. To this end, we obtained gene expression profile data and identified neutrophil-related enriched terms in rupture-prone using Gene Ontology analysis. Next, validate role neutrophils lesions, granulocyte-colony stimulating factor (G-CSF) was administered rat model, which more...
AbstractPurpose: The effects of the heat shock protein 90 (Hsp90) inhibitor geldanamycin (GA) were examined on radiosensitivity and signal transduction pathways in human tumour cell lines.Materials methods: Two lines, SQ‐5 DLD‐1, derived from lung carcinoma colon adenocarcinoma, respectively, incubated for 16 h at 37°C medium containing 0.2 µM GA. cells then irradiated with X‐rays GA a further 8 h. Radiation sensitivity was determined by clonogenic assays levels Western blotting.Results:...
Microcystis strains (2 toxic and 18 nontoxic to mice) were isolated from waterblooms that had been collected Lake Kasumigaura, Ibaraki Prefecture, Japan, in August 1985. Thirteen of the 11 nontoxic) aeruginosa, 2 (nontoxic) wesenbergii, other 5 difficult identify. Six (1 4 M. aeruginosa 1 wesenbergii) these 20 established as axenic cultures. A strain K-139, was used study relationship between growth conditions toxicity. Cells early-to-mid-log phase showed highest toxicity (50% lethal dose,...
The formation of muscle fibers involves sequential expression many proteins that regulate key steps during myoblast‐to‐myotube transition. Myogenin is a major player in the initiation and maintenance myogenic differentiation mouse myoblast cell line, C2C12. RNA‐binding bind to specific target RNA sequences gene post‐transcriptional manner. This study demonstrates motif protein 24 (Rbm24) interacts with 3′‐untranslated region myogenin mRNA affects its half‐life C2C12 myogenesis. Knockdown...
The gene cluster involved in producing the cyclic heptadepsipeptide micropeptin was cloned from genome of unicellular cyanobacterium Microcystis aeruginosa K-139. Sequencing revealed four genes encoding non-ribosomal peptide synthetases (NRPSs) that are highly similar to cyanopeptolins biosynthesis. According predictions based on consensus code, order mcnABCE NPRS modules well consistent with biosynthetic assembly peptides. biochemical analysis a McnB(K-139) adenylation domain and knock-out...
Poly-beta-hydroxybutyrate (PHB) synthesis in a cyanobacterium, Synechococcus sp. strain MA19, is controlled at the enzyme level and dependent on C/N balance culture medium. The control involves least two enzymes. first PHB synthase. Little synthase activity was detected crude extracts from cells grown under nitrogen-sufficient conditions (MA19(+N)). exclusively membrane fractions nitrogen-deprived (MA19(-N)) light but not dark conditions. shift insensitive to chloramphenicol, which suggests...
The sre gene (ORF469) of the R4 phage encodes a protein similar to resolvase-DNA invertase family proteins. Insertional disruption prevented lysogen from entering lytic cycle, implying that Sre is site-specific recombinase needed for excision prophage genome (M. Matsuura, T. Noguchi, Aida, M. Asayama, H. Takahashi, and Shirai, J. Gen. Appl. Microbiol. 41:53-61, 1995). To determine whether this also necessary integration reaction, we studied its function by plasmid analysis. When deletions,...
The Polycomb-group (PcG) gene Rae28 is a mammalian homologue of the Drosophila polyhomeotic. PcG genes are known to maintain transcription states, once initiated, probably by regulating chromatin structure. Since homozygous Rae28-deficient (Rae28–/–) mice displayed cardiac anomalies similar congenital heart diseases in humans, we examined role morphogenesis at molecular level. In Rae28–/– embryos, expression selector Nkx2.5/Csx (Nkx2.5) was initiated properly but not sufficiently sustained...
The paucity of specific surface markers for cardiomyocytes and their progenitors has impeded the development embryonic or pluripotent stem cell-based transplantation therapy. Identification relevant may also enhance our understanding mechanisms underlying differentiation.Here, we show that cellular prion protein (PrP) serves as an effective marker isolating nascent well cardiomyogenic progenitors.Embryonic (or embryo-derived) cells were analyzed using flow cytometry to detect expression PrP...
Breast reconstruction is crucial for patients who have undergone mastectomy breast cancer. Our bioabsorbable implants comprising an outer poly-l-lactic acid mesh and inner component filled with collagen sponge promote retain adipogenesis in vivo without the addition of exogenous cells or growth factors. In this study, we evaluated over time histologically at gene expression level using implant a rodent model. The were inserted inguinal dorsal regions animals. At 1, 3, 6, 12 months...
Abstract Retinitis pigmentosa (RP) and macular dystrophy (MD) cause severe retinal dysfunction, affecting 1 in 4000 people worldwide. This disease is currently assumed to be intractable, because effective therapeutic methods have not been established, regardless of genetic or sporadic traits. Here, we examined a RP mouse model which the Prominin-1 ( Prom1 ) gene was deficient investigated molecular events occurring at outset dysfunction. We extracted Prom1-deficient retina subjected light...
Microcystis aeruginosa is a common cyanobacterium in water blooms that appear widely nutrient-rich, fresh, and brackish waters, its toxic cause the death of domestic animals. The administration crude cell extract M. K-139 to mice can produce tumor necrosis factor (TNF) prompt severe physiological disturbances, especially liver damage, which lead death. vitro production TNF-alpha by peritoneal macrophages was observed after stimulation with or purified toxin from cells. expression mRNA also...
The Polycomb-group (PcG) gene Rae28 is a mammalian homologue of the Drosophila polyhomeotic. PcG genes are known to maintain transcription states, once initiated, probably by regulating chromatin structure. Since homozygous Rae28-deficient (Rae28–/–) mice displayed cardiac anomalies similar congenital heart diseases in humans, we examined role morphogenesis at molecular level. In Rae28–/– embryos, expression selector Nkx2.5/Csx (Nkx2.5) was initiated properly but not sufficiently sustained...
Effective diagnostic biomarkers for aortic aneurysm (AA) that are detectable in blood tests required because early detection and rupture risk assessment of AA can provide insights into medical therapy preventive treatments. However, known lack specificity reliability clinical diagnosis.We performed proteome analysis serum samples from patients with atherosclerotic thoracic (TAA) healthy control (HC) subjects to identify AA. Serum were separated low-density lipoprotein, high-density protein...