A5010744605- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Blood transfusion and management
- Hemoglobinopathies and Related Disorders
- Blood donation and transfusion practices
- Renal Transplantation Outcomes and Treatments
- Blood groups and transfusion
- Acute Myeloid Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Platelet Disorders and Treatments
- Hepatitis B Virus Studies
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- COVID-19 Impact on Reproduction
- Corneal Surgery and Treatments
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Antibiotics Pharmacokinetics and Efficacy
- Erythropoietin and Anemia Treatment
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
Inserm
2017-2025
Université de Bordeaux
2011-2025
Établissement Français du Sang
2016-2025
Bordeaux Population Health
2017-2025
Maladies Rares: Génétique et Métabolisme
2023-2024
Institut National de la Transfusion Sanguine
2023
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2021-2022
Hôpital Pellegrin
2004-2022
Laboratoire Excell
2017
Limousin Regional Council
2007-2016
In normal individuals, γδ T cells account for less than 6% of total peripheral lymphocytes and mainly express T-cell receptor (TCR) Vδ2-Vγ9 chains. We have previously observed a dramatic expansion in the blood renal allograft recipients only when they developed cytomegalovirus (CMV) infection. This increase was long lasting (more 1 year), associated with an activation cells, concerned Vδ1 or Vδ3 subpopulations. Analysis TCR junctional diversity revealed that CMV infection these patients...
Thanks to its unique biological properties, the human amniotic membrane (AM) has shown promising results for guided bone regeneration (GBR), but displays some limitations such as poor space-maintaining ability. This study thus aimed develop a new amnion/chorion (ACM), with better mechanical properties well comparable or improved GBR. We first developed decellularization method of ACM (DL-ACM) which was validated by DNA staining and quantification, cytocompatibility established in vitro. The...
γδ T cells undergo massive expansion in the peripheral blood of renal transplant recipients who are infected with cytomegalovirus (CMV). In a 3-year prospective study, relationship between evolution CMV infection and kinetics cell amplification was followed for 10 months after transplantation. Patients late (⩾45 days) had significantly longer (P<.0001) higher (P<.0003) pp65 antigenemia more-symptomatic disease than did patients early expansion. Analysis data each patient showed that is...
We have characterized the heterogeneity of human blood NK cell subsets defined by expression KIR, lectin like receptors and differentiation markers within a cohort 51 healthy Caucasian individuals. High inter-individual variability in surface most is observed. Range values defining donors were further used as references to characterize 14 patients with NK-type lymphoproliferative disease granular lymphocytes (NK-LDGL). Alterations KIR repertoire noted all NK-LDGL patients. expansions...
The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O(2) concentration would be most favorable for maintenance of hematopoietic stem (HSCs) in course ex vivo expansion grafts. To test this hypothesis, we performed a co-culture cord blood CD34(+) with or without MSC presence cytokines 10 days at 20%, 5%, 1.5% assessed impact on total cells, committed progenitors (colony-forming cells-CFC) activity (pre-CFC Scid repopulating...
We developed a clinical-scale cord blood (CB) cell ex vivo procedure to enable an extensive expansion of committed progenitors—colony-forming cells (CFCs) without impairing very primitive hematopoietic stem (HSCs). CD34 ++ cells, selected from previously cryopreserved and thawed CB units, were cultured in two steps (diluted 1:4 after 6 days) the presence factor (SCF), fms-related tyrosine kinase 3 ligand (Flt-3L), megakaryocyte growth development (MGDF) (100 ng/ml each), granulocyte-colony...
Low O(2) concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both at 20% O(2), we studied its effects in cultures 1% O(2). The pre-CFC activity Lin- population mouse bone marrow was analyzed following 10 days serum-free culture medium (LC1) supplemented with IL-3 without IL-6, 20 phenotypic differentiation proliferative history monitored. receptor expression initiation VEGF-A synthesis were also investigated....
Pharmaceutical development and manufacturing process optimization work was undertaken in order to propose a potential paediatric rectal formulation of azithromycin as an alternative existing oral or injectable formulations. The target product profile be easy-to-use, cheap stable tropical conditions, with bioavailability comparable forms, rapidly achieving maintaining bactericidal concentrations. PEG solid solution suppositories were characterized vitro using visual, HPLC, DSC, FTIR XRD...
To investigate the role of reduced-intensity allogeneic (RIC-allo) stem cell transplant (SCT) as postremission therapy in adult intermediate-risk patients with acute myelogenous leukemia (AML) FLT3-ITD or wild-type NPM1 and CEBPA without FLT3-ITD, we conducted a single-center retrospective study between January 2001 December 2010. Sixty-six were included: 37 treated RIC-alloSCT 29 nonallogeneic SCT therapies. Both groups comparable concerning age, WBC count at diagnosis, gender, karyotype,...
Medication-related osteonecrosis of the jaw (MRONJ) is a complication certain pharmacological treatments such as bisphosphonates, denosumab, and angiogenesis inhibitors. There are currently no guidelines on its management, particularly in advanced stages. The human amniotic membrane (hAM) has low immunogenicity exerts anti-inflammatory, antifibrotic, antimicrobial, antiviral, analgesic effects. It source stem cells growth factors promoting tissue regeneration. hAM acts an anatomical barrier...
Used leuko-depletion filters (LDFs), containing billions of white blood cells (WBCs), are discarded. Because the steady-state contains low quantities stem and progenitor that retained in LDFs, viability functional properties mononuclear (MNCs) CD 34+ recovered from LDFs were investigated.WBCs by use a closed system. MNCs isolated freshly LDF-recovered WBCs or after their overnight incubation. The enumerated, as well number colony-forming unit (CFU)-granulocyte-macrophage, burst-forming...
NK cell receptors for MHC class I molecules (MHC-NKR) can be expressed by T subsets. The restricted repertoire and phenotypic characteristics of MHC-NKR+ cells indicate that expression MHC-NKR is acquired upon antigenic challenge might promote expansion cells. Previous studies performed on in vitro generated αβ clones concluded was not a clonal attribute. Here, we examined massive monoclonal non-leukemic γδ population found the peripheral blood lung-transplanted patient who suffered from...
BACKGROUND: The classification of patients into “good” or “poor” mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte–colony‐stimulating factor (G‐CSF) injection. We hypothesized that, apart from mobilization marrow to the blood, response G‐CSF cells also includes activation proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB purified samples obtained by cytapheresis multiple myeloma non‐Hodgkin's lymphoma...
Perinatal derivatives are drawing growing interest among the scientific community as an unrestricted source of multipotent stromal cells, stem cellular soluble mediators, and biological matrices. They useful for treatment diseases that currently have limited or no effective therapeutic options by means developing regenerative approaches. In this paper, to generate a complete view state art, comprehensive 10-years compilation clinical-trial data with common denominator PnD usage has been...
BACKGROUND: Mobilized PBPCs, detectable on the basis of CD34 expression, can be collected various cell separators. The CD34+ collection efficiencies two separators (CS‐3000+ and Amicus, Baxter) were tested comparable groups oncology patients. STUDY DESIGN AND METHODS: Leukapheresis assisted by standard manufacturer's software variables settings was performed in 37 (CS‐3000+) 34 (Amicus) patients (total 83 67 collections, respectively) after chemotherapy plus G‐CSF treatment. RESULTS: total...
Abstract Matching for HLA‐A, ‐B, ‐C, and ‐DRB1 loci (8/8 match) is currently the gold standard unrelated donor hematopoietic cell transplantation (HCT). In Europe, patients are also matched at HLA‐DQB1 (10/10 match). However, there increasing evidence that matching HLA‐DRB3/4/5 may help to lower transplant‐related morbidity mortality. We therefore investigated impact of mismatches on outcomes in 1975 who received a first 10/10 (MUD) HCT France from 2000 2012 hematological malignancy....
We performed a retrospective study to identify pretransplantation risk factors for steroid-refractory (SR) acute graft-versus host disease (aGVHD) after allogeneic stem cell transplantation from matched donors in 630 adult patients who underwent at our center between 2000 and 2012. The cumulative incidence (CI) of SR aGVHD was 11.3% ± 2.3%. identified independent were unrelated donor (hazard ratio [HR], 2.52; P = .001), female male recipient (HR, 1.84; .023) absence antithymocyte globulin...