A5036819913- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- CRISPR and Genetic Engineering
- Respiratory viral infections research
- DNA Repair Mechanisms
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- RNA Research and Splicing
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- COVID-19 and Mental Health
- Long-Term Effects of COVID-19
- Autoimmune and Inflammatory Disorders Research
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Protist diversity and phylogeny
- Planetary Science and Exploration
- IL-33, ST2, and ILC Pathways
- Listeria monocytogenes in Food Safety
- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- Metalloenzymes and iron-sulfur proteins
Klinikum Dortmund
2024
Umeå University
2021-2022
Newcastle University
2017-2019
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2016
University of Sussex
1991
Bacterial RNA polymerase is able to initiate transcription with adenosine-containing cofactor NAD+, which was proposed result in a portion of cellular RNAs being ‘capped’ at the 5′ end reminiscent eukaryotic cap. Here we show that, apart from another FAD and highly abundant uridine-containing cell wall precursors, UDP-Glucose UDP-N-acetylglucosamine are efficiently used vitro. We that affinity NAD+ UDP-containing factors during initiation much lower than their concentrations, them stimulates...
IL-22 plays a critical role in defending against mucosal infections, but how production is regulated incompletely understood. Here, we show that mice lacking IL-33 or its receptor ST2 (IL-1RL1) were more resistant to Streptococcus pneumoniae lung infection than wild-type animals and single-nucleotide polymorphisms IL33 IL1RL1 associated with pneumococcal pneumonia humans. The effect of on S. was mediated by negative regulation innate lymphoid cells (ILCs) independent ILC2s as well IL-4 IL-13...
Community-acquired pneumonia (CAP) is a major global cause of death and hospitalization. Bacteria or community-acquired viruses (CARVs) CAP. COVID-19 associated restrictions effectively reduced the circulation CARVs. The aim this study was to analyze proportion CARVs in adult patients with CAP from mid-2020 mid-2023. Specifically, we aimed compare rate influenza virus, SARS-CoV-2, RSV detections aged 18–59 years ≥60 years. We 21 respiratory three atypical bacteria (Bordetella pertussis,...
HflX is a ubiquitous bacterial GTPase that splits and recycles stressed ribosomes. In addition to HflX, Listeria monocytogenes contains second homolog, HflXr. Unlike HflXr confers resistance macrolide lincosamide antibiotics by an experimentally unexplored mechanism. Here, we have determined cryo-EM structures of L. HflXr-50S HflX-50S complexes as well 70S ribosomes in the presence absence lincomycin. While overall geometry on 50S subunit similar loop within N-terminal domain HflXr, which...
Stringent factors orchestrate bacterial cell reprogramming through increasing the level of alarmones (p)ppGpp. In Beta- and Gammaproteobacteria, SpoT hydrolyzes (p)ppGpp to counteract synthetase activity RelA. However, structural information about how controls levels is missing. Here we present crystal structure hydrolase-only from Acinetobacter baumannii uncover mechanism intramolecular regulation 'long'-stringent factors. contrast ribosome-associated Rel/RelA that adopt an elongated...
Abstract Purpose Coronavirus disease 2019 (COVID-19) and non-COVID-19 community-acquired pneumonia (NC-CAP) often result in hospitalization with considerable risks of mortality, ICU treatment, long-term morbidity. A comparative analysis clinical outcomes COVID-19 CAP (C-CAP) NC-CAP may improve management. Methods Using prospectively collected CAPNETZ study data (January 2017 to June 2021, 35 centers), we conducted a comprehensive including in-hospital death, length hospital stay (LOHS),...
In times of increasing space exploration and search for extraterrestrial life, new questions challenges planetary protection, aiming to avoid forward contamination different planets or moons with terrestrial are emerging. Spore-forming bacteria such as Bacillus species have a high potential due their spores' extreme resistance, enabling them withstand conditions. Spores require liquid water conversion into growing cell (i.e., spore germination subsequent growth). If present, on is likely be...
The steady-state kinetics of reductant-independent ATP hydrolysis by Klebsiella pneumoniae nitrogenase at 23 degrees C pH 7.4 were determined as a function component protein ratio (optimal an oxidized Fe protein/MoFe 3:1) and MgATP concentration (Km 400 microM). Competitive inhibition was observed for MgADP (Ki 145 microM), [beta gamma-methylene]ATP (Mgp[CH2]ppA) 115 gamma-monofluoromethylene]ATP (Mgp[CHF]ppA) 53 microM) gamma-difluoromethylene]ATP (Mgp[CF2]ppA) 160 tighter binding to free...
To initiate replication on a double-stranded DNA de novo, all organisms require primase, an RNA polymerase making short primers which are then extended by polymerases. Here, we show that primase can use metabolic cofactors as initiating substrates, instead of its canonical substrate ATP. DnaG Escherichia coli initiates synthesis with NADH (the reduced form nicotinamide adenine dinucleotide) and FAD (flavin in vitro. These consist ADP core covalently bound to extra moieties. The component...
Abstract Recently a new, non-canonical type of 5’-RNA capping with cellular metabolic cofactors was discovered in bacteria and eukaryotes. This is performed by RNA polymerases, the main enzymes transcription, which initiate synthesis cofactors. Here we show that primase, enzyme replication primes DNA making short primers, initiates primers using number Primase DnaG E. coli starts NAD + /NADH, FAD DP-CoA vitro . activity does not affect primase specificity initiation. ppGpp, global starvation...
Abstract Stringent factors orchestrate bacterial cell reprogramming through increasing the level of alarmones (p)ppGpp. In Beta- and Gammaproteobacteria, SpoT hydrolyses (p)ppGpp to counteract synthetase activity RelA. However, structural information about how controls levels is missing. Here we present crystal structure hydrolase-only from Acinetobacter baumannii uncover mechanism intra-molecular regulation “long”-RSH factors. contrast ribosome-associated Rel/RelA that adopt an elongated...