A5004514114- Nerve injury and regeneration
- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Advanced Neuroimaging Techniques and Applications
- Spinal Dysraphism and Malformations
- Pluripotent Stem Cells Research
- Neurogenetic and Muscular Disorders Research
- Cerebral Palsy and Movement Disorders
- Traumatic Brain Injury Research
- Pain Mechanisms and Treatments
- Neuroscience and Neural Engineering
- Functional Brain Connectivity Studies
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Bacterial Genetics and Biotechnology
- 3D Printing in Biomedical Research
- DNA Repair Mechanisms
- Adenosine and Purinergic Signaling
- Neonatal and fetal brain pathology
- EEG and Brain-Computer Interfaces
- Cancer, Stress, Anesthesia, and Immune Response
- Prenatal Screening and Diagnostics
- Transcranial Magnetic Stimulation Studies
Kennedy Krieger Institute
2012-2024
Johns Hopkins Medicine
2011-2022
Johns Hopkins University
2012-2022
University of Oklahoma Health Sciences Center
2001-2006
Oklahoma Medical Research Foundation
2001-2006
Resting-state functional MRI (rs-fMRI) permits study of the brain's networks without requiring participants to perform tasks. Robust changes in such resting state (RSNs) have been observed neurologic disorders, and rs-fMRI outcome measures are candidate biomarkers for monitoring clinical trials, including trials extended therapeutic interventions rehabilitation patients with chronic conditions. In this study, we aim present a unique longitudinal dataset reporting on healthy adult subject...
Abstract Damage to specific white matter tracts within the spinal cord can often result in particular neurological syndromes that characterize myelopathies such as traumatic injury. Noninvasive visualization of these with imaging techniques are sensitive microstructural integrity is an important clinical goal. Diffusion tensor (DTI)‐ and magnetization transfer (MT)‐derived quantities have shown promise assessing tissue health central nervous system. In this paper, we demonstrate DTI cervical...
Neurological recovery in patients with severe spinal cord injury (SCI) is extremely rare. We have identified a patient chronic cervical traumatic SCI, who suffered complete loss of motor and sensory function below the for 6 weeks after injury, but experienced progressive neurological that continued 17 years. The extent patient's from trauma-induced paralysis rare remarkable. A detailed study this using diffusion tensor imaging (DTI), magnetization transfer (MTI), resting state fMRI (rs-fMRI)...
We sought to investigate the effects of co-grafting neural stem cells (NSCs) with olfactory ensheathing (OECs) on neurological behavior in rats subjected traumatic brain injury (TBI) and explore underlying molecular mechanisms. TBI was established by percussion device made through a weight drop (50 g) from 30 cm height. Cultured NSCs OECs isolated were labeled Hoechst 33342 (blue) chloromethyl-benzamidodialkyl carbocyanine (CM-Dil) (red), respectively. Then, and/or OECs, separately or...
Introduction: Spinal cord injury (SCI) causes partial or complete damage to sensory and motor pathways induces immediate changes in cortical function. Current rehabilitative strategies do not address this early alteration, therefore impacting the degree of neuroplasticity subsequent recovery. The following study aims test if a non-invasive brain stimulation technique such as repetitive transcranial magnetic (rTMS) is effective promoting plasticity rehabilitation, can be used an intervention...
Chronic neuropathic pain is a common and debilitating consequence of spinal cord injury (SCI). In rat contusion model, we observed that chronic present on day 7 after SCI persists for the entire 56-day observation period. However, currently available therapies are inadequate SCI-induced pain. this study, show transplantation mouse embryonic stem cell-derived oligodendrocyte progenitor cells (OPCs) enhances remyelination in injured reduces Moreover, found protein level neuregulin-1 ErbB4 OPC...
Abstract Patients with tumors that metastasize to bone frequently suffer from debilitating pain, and effective therapies for treating cancer are lacking. This study employed a novel strategy in which herpes simplex virus (HSV) carrying small interfering RNA (siRNA) targeting platelet-derived growth factor (PDGF) was used alleviate pain. HSV PDGF siRNA established intrathecally injected into the cavum subarachnoidale of animals suffering pain negative group. Sensory function assessed by...
Myelination in the central nervous system (CNS) is a dynamic process that includes birth of oligodendrocyte progenitor cells (OPCs), their differentiation into oligodendrocytes, and ensheathment axons. Regulation myelination by neuronal activity has emerged as new mechanism CNS plasticity. Activity-dependent been shown to regulate sensory, motor, cognitive functions. In this work, we aimed employ plasticity utilizing induced promote remyelination functional recovery subchronic model spinal...
Developmental hemoglobin switching involves sequential globin gene activations and repressions that are incompletely understood. Earlier observations, described herein, led us to hypothesize nuclear ferritin is a repressor of the adult beta-globin in embryonic erythroid cells. Our data show ferritin-family protein K562 cell extracts binds specifically highly conserved CAGTGC motif promoter at -153 -148 bp from cap site, mutation reduces binding 20-fold competition gel-shift assays. Purified...
Abstract Spinal cord injury (SCI) is frequently accompanied by a degree of spontaneous functional recovery. The underlying mechanisms through which such recovery generated remain elusive. In this study, we observed significant motor function 14 to 28 days after spinal transection (SCT) in rats. Using comparative proteomics approach, caudal the injury, detected difference 20 proteins. Two these proteins, are eukaryotic translation initiation factor 5A1 (eIF5A1) that involved cell survival and...
Neonatal hypoxic-ischemic encephalopathy (NHIE) induces severe cerebral damage and neurological dysfunction, with seldom effective therapy. Aquaporin-4 (AQP4) is involved in aggravating brain induced by NHIE. This study aimed to investigate the role of AQP4 underlying pathogenesis Sprague-Dawley rats were used establish neonatal (HI) models, expression cortex, hippocampus, lung tissues was detected real-time quantitative polymerase chain reaction as well Western blot. Primary cortical...
Spinal cord injury (SCI) is a fatal disease that can cause severe disability. Cortical reorganization subserved the recovery of spontaneous function after SCI, although potential molecular mechanism in this remote control largely unknown. Therefore, using proteomics analysis, RNA interference/overexpression, and CRISPR/Cas9 vivo vitro , we analyzed how network functions neurological improvement, especially motor spinal transection (SCT) via regulation cerebral cortex. We discovered...
Neurological recovery in individuals with spinal cord injury (SCI) is multifaceted, involving mechanisms such as remyelination and perilesional neuroplasticity, cortical reorganization being one contributing factor. Cortical reorganization, particular, can be evaluated through network (graph) analysis of interregional functional connectivity. This study aimed to investigate patterns persons chronic SCI using a multilayer community detection approach on resting-state MRI data. Thirty-eight...
The use of cell fusion to study exchange information at the molecular level between nucleus and cytoplasm cells during regulation gene expression was pioneered by Harris Ringertz more than three decades ago. ability make heterokaryons with from different species or genetic strains is especially useful because differences in products allow origin trans-acting regulatory factors be determined. Heterokaryons adult nucleated erythroid one embryonic/larval another species, for example, show...
A platform for studying spinal cord organogenesis in vivo where embryonic stem cell (ESC)-derived neural progenitor cells (NPC) self-organize into cord-like tissue after transplantation subarachnoid space of the has been described. We advance applicability this by imaging formed graft through T2w magnetic resonance (MRI). Furthermore, we used diffusion tensor (DTI) to verify stereotypical organization showing that it mimics host cord. Within white matter (WM) identified astrocytes form glial...