A5082251798- Osteoarthritis Treatment and Mechanisms
- Gastroesophageal reflux and treatments
- Knee injuries and reconstruction techniques
- Silk-based biomaterials and applications
- Esophageal and GI Pathology
- Periodontal Regeneration and Treatments
- Total Knee Arthroplasty Outcomes
- Tracheal and airway disorders
- Pancreatitis Pathology and Treatment
- Eosinophilic Esophagitis
- Esophageal Cancer Research and Treatment
- Foreign Body Medical Cases
- Tendon Structure and Treatment
- Pancreatic function and diabetes
- Gastrointestinal Bleeding Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Proteoglycans and glycosaminoglycans research
- Helicobacter pylori-related gastroenterology studies
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Gastrointestinal disorders and treatments
- Congenital Diaphragmatic Hernia Studies
- Lung Cancer Research Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Appendicitis Diagnosis and Management
Johns Hopkins University
2018-2025
Johns Hopkins Medicine
2019-2025
UNSW Sydney
2025
Johns Hopkins Children's Center
2018-2024
SUNY Downstate Health Sciences University
2017
State University of New York
2017
Baylor College of Medicine
2016
Texas Children's Hospital
2016
Hospital for Special Surgery
2010-2013
Northwell Health
2012
Inspired by the depth-dependent inhomogeneity of articular cartilage, it was hypothesized that a novel layered agarose technique, using 2% (wt/vol) top and 3% bottom layer, would create an inhomogenous tissue construct with distinct material properties in conjoined regions. The biochemical mechanical development these constructs observed alongside uniform constructs. Initially, disks had highest bulk Young's modulus (E(Y) approximately 28 kPa) all groups. After days culture 20%...
We hypothesized that zonal populations of chondrocytes seeded into a bilayered scaffold with initially prescribed depth-varying, compressive material properties will lead to biomimetic cartilage tissue construct depth-dependent cellular and mechanical inhomogeneity similar the native tissue. Superficial zone (SZCs) middle/deep (MDZCs) were isolated encapsulated 2% or 3% agarose form single-layered constructs SZC, MDZC; SZC/2% MDZC mixed chondrocyte controls. For SZCs on day 42, increased...
It was hypothesized that previously optimized serum-free culture conditions for juvenile bovine chondrocytes could be adapted to generate engineered cartilage with physiologic mechanical properties in a preclinical, adult canine model. Primary or passaged (using growth factors) from three dogs were encapsulated agarose, and cultured media transforming factor-beta3. After 28 days culture, formed by primary exhibited only small increases glycosaminoglycan content. However, all on day...
Among children who suffer from acute recurrent pancreatitis (ARP) or chronic (CP), (AP) episodes are painful, often require hospitalization, and contribute to disease complications progression. Despite this recognition, there currently no interventions prevent AP episodes. In retrospective cohort study, we assessed the impact of pancreatic enzyme therapy (PERT) use on clinical outcomes among with pancreatic-sufficient ARP CP.
Abstract Background and Aims The Eosinophilic Esophagitis Histology Scoring System (EoEHSS) is useful for diagnosing characterizing eosinophilic esophagitis (EoE). A limitation of the EoEHSS that lamina propria fibrosis scores are infrequently determined due to challenges in sampling propria. Low distensibility index (DI) measured by endoluminal functional lumen imaging probe (EndoFLIP) associated with fibrostenotic severity pediatric patients EoE. We investigated correlation between DI...
Chronic pancreatitis is a fibro-inflammatory syndrome of the pancreas with prevalence in children approximately 6 per 100,000. Diagnosis chronic child depends on presence imaging findings context specific clinical features. Currently, standardized reporting system for lacking, and imaging-based thresholds abnormality have not been defined. Standardized elements were defined adults 2019. Not all these criteria are directly applicable to due recognized changes normal growth development. To...
Due to the prevalence of osteoarthritis (OA) and damage articular cartilage, coupled with poor intrinsic healing capacity this avascular connective tissue, there is a great demand for an cartilage substitute. As bearing material diarthrodial joints, has remarkable functional properties that have been difficult reproduce in tissue‐engineered constructs. We previously demonstrated by using tissue engineering approach incorporates mechanical loading into long‐term culture environment, one can...
Abstract To better understand the effects of scaffold materials for bone morphogenetic protein 2 (BMP‐2) genetic tissue engineering in vivo , several gels, including alginate, collagen, agarose, hyaluronate, fibrin, or Pluronic, were mixed with adenovirus‐mediated human BMP‐2 gene (Adv‐hBMP‐2) transduced marrow stromal cells (BMSCs) and injected into muscles athymic mice to evaluate resulting osteogenesis chondrogenesis. These gel gene‐transduced BMSC mixtures also loaded onto β‐TCP/HAP...
A fundamental challenge of cartilage tissue engineering has been the inability to promote collagen synthesis up native levels. In contrast, recent protocols have demonstrated that glycosaminoglycans (GAG) can be synthesized levels in 4-6 weeks vitro culture. We hypothesize rapid GAG may an impediment synthesis, possibly by altering transport pathways nutrients or products. this study, hypothesis is tested inducing enzymatic loss early culture period constructs, and monitoring content at...
BACKGROUND AND OBJECTIVES High-powered magnets were effectively removed from the US market by Consumer Product Safety Commission (CPSC) in 2012 but returned 2016 after federal court decisions. The United States Court of Appeals for 10th Circuit cited imprecise data among other reasons as justification overturning CPSC protections. Since then, incidence high-powered magnet exposure has increased markedly, outcome are limited. In this study, we aim to describe epidemiology and outcomes...
Focal cartilage defects reduce the ability of articular to resist mechanical loading and provide lubrication during joint motion. The limitations in current surgical treatments have motivated use biocompatible scaffolds as a future treatment option. Here we describe second generation macroporous, polyvinyl alcohol (PVA) scaffold with independently tunable morphological properties. compressive moduli PVA increased increasing polymer concentration applied strain, values range for human (HA >...
Scaffold-cartilage integration is critical for the clinical success of a scaffold used repair focal cartilage defect. In this study, macroporous polyvinyl alcohol (PVA) was found to facilitate chondrocyte infiltration and interfacial matrix formation in juvenile bovine vitro defect model. These results were depend on press-fit between cartilage, pretreatment with collagenase prior insertion, preseeding scaffold. Infiltrated preseeded chondrocytes survived 6 weeks culture resulted sufficient...
Abstract Few options exist to replace or repair damaged articular cartilage. The optimal solution that has been suggested is a scaffold can carry load and integrate with surrounding tissues; but such construct thus far elusive. objectives of this study were manufacture characterize nondegradable hydrated scaffold. Our hypothesis was the polymer content be used control its mechanical properties, while an internal porous network augmented biological agents facilitate integration host tissue....
The application of dynamic physiologic loading to a bilayered chondrocyte-seeded agarose construct with 2% (wt/vol) top layer and 3% bottom was hypothesized (1) improve overall properties (2) result in tissue that mimics the mechanical inhomogeneity native cartilage. Dynamic over 28 day culture period found significantly increase bulk biochemical versus free-swelling culture. initial depth-distribution compressive Young's modulus (EY) reflected intrinsic gel each similar trend tissue, softer...