A5058681844- Cystic Fibrosis Research Advances
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Gut microbiota and health
- Pediatric health and respiratory diseases
- Mycobacterium research and diagnosis
- Tracheal and airway disorders
- Tuberculosis Research and Epidemiology
- Bacterial biofilms and quorum sensing
- Neonatal Respiratory Health Research
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Infectious Diseases and Tuberculosis
- Plant Pathogens and Fungal Diseases
- Inhalation and Respiratory Drug Delivery
- Indoor Air Quality and Microbial Exposure
- Antimicrobial Resistance in Staphylococcus
- Dysphagia Assessment and Management
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Respiratory Support and Mechanisms
Nanyang Technological University
2017-2024
Trinity College Dublin
2014-2024
St. James's Hospital
2014-2024
University of Dundee
2022
University of Oxford
2021
University of Tasmania
2019
La Trobe University
2019
Molina Center for Energy and the Environment
2018
National University of Singapore
2018
University College Cork
2009-2012
Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance cellular has been shown mice, T cell responses have not characterised. Using a murine model recurrent S. peritonitis, we demonstrated that prior exposure enhanced IFNγ upon subsequent infection, while adoptive transfer antigen-specific Th1 cells was naïve mice. Translating these findings, found were also significantly expanded during human bloodstream (BSI). These CD45RO+,...
Understanding the composition and clinical importance of fungal mycobiome was recently identified as a key topic in “research priorities” consensus statement for bronchiectasis. Patients were recruited part CAMEB study: an international multicentre cross-sectional Cohort Asian Matched European Bronchiectasis patients. The determined 238 patients by targeted amplicon shotgun sequencing 18S–28S rRNA internally transcribed spacer regions ITS1 ITS2. Specific quantitative PCR detection conidial...
Rationale: Long-term antibiotic use for managing chronic respiratory disease is increasing; however, the role of airway resistome and its relationship to host microbiomes remains unknown.Objectives: To evaluate resistomes relate them environmental using ultradeep metagenomic shotgun sequencing.Methods: Airway specimens from 85 individuals with without (severe asthma, obstructive pulmonary disease, bronchiectasis) were subjected sequencing an average depth exceeding 20 million reads....
Rationale: Emerging data support the existence of a microbial "gut-lung" axis that remains unexplored in bronchiectasis. Methods: Prospective and concurrent sampling gut (stool) lung (sputum) was performed cohort n = 57 individuals with bronchiectasis subjected to bacteriome (16S rRNA) mycobiome (18S Internal Transcribed Spacer) sequencing (total, 228 microbiomes). Shotgun metagenomics subset (n 15; 30 Data from compartments were integrated by weighted similarity network fusion, clustered,...
Neisseria species are frequently identified in the bronchiectasis microbiome, but they regarded as respiratory commensals. Using a combination of human cohorts, next-generation sequencing, systems biology, and animal models, we show that bacteriomes defined by presence spp. associate with poor clinical outcomes, including exacerbations. subflava cultivated from patients promotes loss epithelial integrity inflammation primary cells. In vivo models infection metabolipidome analysis highlight...
Chronic infection and inflammation shapes the airway microbiome in bronchiectasis. Utilizing whole-genome shotgun metagenomics to analyze resistome provides insight into interplay between microbes, resistance genes, clinical outcomes.
Progressive lung function loss is recognized in chronic obstructive pulmonary disease (COPD); however, no study concurrently evaluates how accelerated decline relates to mucus properties and the microbiome COPD.
The LysR-family regulator MexT modulates the expression of MexEF-OprN efflux system in human pathogen Pseudomonas aeruginosa . Recently, we demonstrated that regulates certain virulence phenotypes, including type-three secretion and early attachment independent its role regulating MexEF-OprN. In this study, transcriptome profiling was utilized to investigate global nature regulation P. PAO1 an isogenic mexEF mutant. Twelve genes unknown function were highly induced by overexpressing A...
Allergic sensitization is associated with poor clinical outcomes in asthma, chronic obstructive pulmonary disease, and cystic fibrosis; however, its presence, frequency, significance non-cystic fibrosis bronchiectasis remain unclear.To determine the frequency geographic variability that exists a pattern to common specific allergens, including house dust mite fungi, correlate such patterns airway immune-inflammatory status bronchiectasis.Patients were recruited Asia (Singapore Malaysia)...
The chronic obstructive pulmonary disease (COPD) bacteriome associates with severity, exacerbations and mortality. While COPD patients are susceptible to fungal sensitisation, the role of mycobiome remains uncertain.We report largest multicentre evaluation airway date, including participants from Asia (Singapore Malaysia) UK (Scotland) when stable (n=337) during (n=66) as well nondiseased (healthy) controls (n=47). Longitudinal analysis was performed following (n=34), examined in terms...
Introduction Allergic sensitisation to fungi such as Aspergillus are associated poor clinical outcomes in asthma, bronchiectasis and cystic fibrosis; however, relevance COPD remains unclear. Methods Patients with stable (n=446) nondiseased controls (n=51) were prospectively recruited across three countries (Singapore, Malaysia Hong Kong) screened against a comprehensive allergen panel including house dust mites, pollens, cockroach fungi. For the first time, using metagenomics approach, we...
Bronchiectasis is marked by bronchial dilatation, recurrent infections and significant morbidity, underpinned a complex interplay between microbial dysbiosis immune dysregulation. The identification of distinct endophenotypes have refined our understanding its pathogenesis, including heterogeneous disease mechanisms that influence treatment prognosis responses. Next-generation sequencing (NGS) has revolutionised the way we view airway microbiology, allowing insights into “unculturable”....
ABSTRACT MexT is a global LysR transcriptional regulator known to modulate antibiotic resistance and virulence in Pseudomonas aeruginosa . In this study, novel role for mediating intrinsic disulfide stress was demonstrated, representing the first identified phenotype associated with inactivation of wild-type cells. Disruption mexT resulted increased susceptibility elicitor diamide [diazenedicarboxylic acid bis( N , ,-di-methylamide)]. This compound elicit specific response via depletion...
Bacterial whole genome sequencing (WGS) is becoming a widely-used technique in research, clinical diagnostic, and public health laboratories. It enables high resolution characterization of bacterial pathogens terms properties that include antibiotic resistance, molecular epidemiology, virulence. The introduction next-generation instrumentation has made WGS attainable costs. However, the lack beginner’s protocol for still represents barrier to its adoption some settings. Here, we...
Objective To investigate age-associated changes in airway microbiome composition and their relationships with lung function arterial stiffness among genetically matched young elderly pairs. Methods Twenty-four linked family pairs comprised of younger (≤40 years) older (≥60 healthy participants were recruited (Total n = 48). Lung (carotid-femoral pulse wave velocity (PWV) augmentation index (AIx)) assessed. Sputum samples collected for targeted 16S rRNA gene amplicon sequencing correlations...