A5021736992- Chemokine receptors and signaling
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Lymphoma Diagnosis and Treatment
- Genetic factors in colorectal cancer
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Head and Neck Cancer Studies
- Bone and Dental Protein Studies
- Gastric Cancer Management and Outcomes
- PI3K/AKT/mTOR signaling in cancer
- Cancer Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
- BRCA gene mutations in cancer
- Neuroendocrine regulation and behavior
- Colorectal Cancer Treatments and Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Multiple and Secondary Primary Cancers
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Neuroscience of respiration and sleep
Universidade de São Paulo
2010-2025
Instituto do Câncer do Estado de São Paulo
2012-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2005-2023
Cancer Institute (WIA)
2022
Laboratory of Molecular Genetics
2022
Hospital Heliópolis
2004-2006
Universidade Federal de São Paulo
2005-2006
ADAMTS-1 (a disintegrin and metalloprotease with thrombospondin motifs) is a member of the ADAMTS family metalloproteases. Here, we investigated mRNA protein levels in normal neoplastic tissues using qPCR, immunohistochemistry immunoblot analyses, addressed role regulating migration, invasion invadopodia formation breast tumor cell lines. In series primary tumors, observed variable expression but lower human cancers as compared to tissue, striking decrease high-malignancy cases...
OBJECTIVES:The phosphatidylinositol 3-kinase/AKT axis is an important cell-signaling pathway that mediates cell proliferation and survival, two biological processes regulate malignant growth.The 3kinase CA gene encodes the p110a subunit of 3-kinase protein.There are mutations in several types human tumors, they frequently observed breast cancer.However, these have not been investigated Brazilian cancer patients.METHODS: PCR-SSCP direct DNA sequencing were performed to identify 3-kinaseCA...
Background: DNAJC12 (DnaJ heat shock protein family (Hsp40) member C12) encodes a of the molecular chaperone Hsp40/DnaJ family, which are important folding and proteostasis regulators. Its role as biomarker has been studied for limited number cancer types. Objectives: Here, we sought to investigate potential mRNA expression prognostic predictive breast (BC). Methods: Using in silico analysis data from immunohistochemistry (IHC) 292 samples patients with primary BC, determined pattern value...
The TNM Classification of Malignant Tumours (TNM) staging system is the primary means determining a prognosis for gastric adenocarcinoma (GC). However, tumor behavior in individual patient unpredictable and spite treatment advances, classification 'advanced stage' still portends poor prognosis. Thus, further insights from molecular analyses are needed better prognostic stratification determination new therapeutic targets.A total fifty-one fresh frozen samples patients with...
Genetic analysis of sporadic medullary thyroid carcinoma (MTC) has revealed somatic variants in
Summary The activating protein‐1 (AP‐1) family of transcription factors has been implicated in the control proliferation and differentiation keratinocytes, but its role malignant transformation is not clear. aim this study to assess pattern mRNA expression jun‐fos AP‐1 members 45 samples head neck squamous cell carcinomas (HNSCC) matched adjacent mucosa by means Northern blot analysis. Transcripts all were identified, except for JunB that was detected only reverse polymerase chain reaction....
The Ataxia-telangiectasia mutated (ATM) gene encodes a multifunctional kinase, which is linked to important cellular functions. Women heterozygous for ATM mutations have an estimated relative risk of developing breast cancer 3.8. However, the pattern and their role in etiology has been controversial remains unclear. In present study, we investigated frequency spectrum series sporadic cancers controls from Brazilian population.Using PCR-Single Strand Conformation Polymorphism (SSCP) analysis...
Juvenile nasopharingeal angiofibroma (JNA) is a histologically benign locally aggressive tumor characterized by irregular vessels embedded in fibrous stroma. Excessive vascularity results bleeding complications, and the inhibition of angiogenesis promising strategy for managing extensive JNA tumors. To better characterize endothelial components JNA, we aimed to evaluate markers vascular differentiation proliferation, such as friend leukemia integration-1 (FLI-1) endoglin, lymphatic markers,...
ABSTRACT Introduction DNA methylation regulates exercise-induced changes in the skeletal muscle transcriptome. However, specificity and time course responses myogenic regulatory factors mRNA expression after divergent exercise modes are unknown. Purpose This study aimed to compare for selected ( MYOD1 , MYF5 MYF6 ) immediately after, 4 h 8 a single bout of resistance (RE), high-intensity interval (HIIE), concurrent (CE). Methods Nine healthy but untrained males (age, 23.9 ± 2.8 yr; body...
To explore the prognostic role of plasma levels osteopontin (OPN), a phosphoglycoprotein with adhesive properties, in patients head and neck squamous cell carcinoma (HNSCC) undergoing concomitant chemoradiotherapy. Previous studies have proposed OPN level as factor several cancers.Prospective analysis levels, before within 12 weeks after treatment, cohort HNSCC platinum-based chemoradiotherapy at our center.Academic center.Sixty-nine diagnosed having HNSCC.Plasma were assessed start...
Idiopathic pulmonary artery hypertension (IPAH), chronic thromboembolic (CTEPH), and acute embolism (APTE) are life-threatening cardiopulmonary diseases without specific surgical or medical treatment. Although APTE, CTEPH IPAH different vascular in terms of clinical presentation, prevalence, pathophysiology prognosis, the identification their circulating microRNA (miRNAs) might help recognizing differences outcome evolution forms. The aim this study was to describe CTEPH, IPAH-associated...
Previous knowledge of cervical lymph node compromise may be crucial to choose the best treatment strategy in oral squamous cell carcinoma (OSCC). Here we propose a set four genes, whose mRNA expression primary tumor predicts nodal status OSCC, excluding tongue.We identified differentially expressed genes OSCC with and without compromised nodes using Differential Display RT-PCR. Known were chosen validated by means Northern blotting or real time RT-PCR (qRT-PCR). Thereafter constructed Nodal...
Aim: The PHLDA (pleckstrin homology like domain, family A) gene encodes proteins capable of inhibiting AKT (serine/threonine kinase) signaling through phosphoinositol binding competition. Results & methodology: Using in silico analysis, we found that Luminal A and B patients' short relapse-free survival was associated with low PHLDA1 or PHLDA3 high PHLDA2 expression. In a cohort 393 patients luminal breast cancer evaluated by immunohistochemistry on tissue microarrays, direct association...
Abnormal long non-coding RNAs (lncRNAs) expression has been documented to have oncogene or tumor suppressor functions in the development and progression of cancer, emerging as promising independent biomarkers for molecular cancer stratification patients’ prognosis. Examining relationship between lncRNAs survival rates malignancies creates new scenarios precision medicine targeted therapy. Breast (BRCA) is a heterogeneous malignancy. Despite advances its classification, there are still gaps...
Category: Thoracic Oncology Introduction: Genomic mutational profiling, obtained through next-generation sequencing (NGS) plays a pivotal role in assisting clinicians therapeutic decision- making patients with non-small cell cancer (NSCLC).(,) However, these techniques require high quality and sufficient quantity of material to produce reliable results.(,) Our study proposes the use tissue scraping from histological cytological slides as an alternative method for obtaining genomic DNA (gDNA)...
Personalized therapy in lung cancer (LC) has revolutionized routine histopathology and cytopathology, emphasizing the importance of obtaining adequate material for molecular studies to support oncological decisions. Adaptations cytologic sample preparations offer benefits testing, yet their potential remains underutilized. A significant number LC cases is identified through specimens aspiration or exfoliative cytology. Improving screening approaches optimizing tissue utilization biomarker...
Aim: To determine the expression signature of triple-negative breast cancer (TNBC) with differences secreted protein acidic and rich in cysteine clinical behavior. Patients, materials & methods: cDNA microarray analysis was performed to profiling TNBC, characterized regarding status. Immunohistochemistry on tissue microarrays containing an independent cohort TNBC for validation. Results: Negative staining SOHLH2 positive DNAJC12 LIM1 correlated a poor outcome patients. Conclusion: Our...
Background Prenatal cocaine exposure (PCE) is associated with behavioral, cognitive, and social consequences in children that might persist into later development. However, there are still few data concerning epigenetic mechanisms the effects of gestational exposure, particularly human newborns. Aims We investigated PCE on DNA methylation patterns Oxytocin Receptor ( OXTR ) gene umbilical cord blood (UCB). The relationship between UCB levels severity mother’s use during pregnancy was also...
Abstract The prostate apoptosis response-4 (PAR4) gene encodes a 38kDa proapoptotic protein with tumor cell selective action and/or sensitization to an additional insult. This apoptotic ability is associated PAR4 nuclear translocation and phosphorylation state. PAR-4 acts through intrinsic extrinsic pathways. can also be secreted by normal/immortalized cancer cells via the classical ER stress pathway mediated GRP78. previous study from our group provided evidence that MCF10A cultured in...
Abstract Genetic instability commonly found in tumors may be a consequence of imbalance homologous recombination (HR), mechanism DNA double-strand break (DSB) repair dependent on the formation and resolution Holliday junctions’ structures for genomic stability maintenance. The SMC6 is involved DSB repair. gene structurally located very close to GEN1 head-to-head organization sharing bi-directional promoter, encoded protein also HR. Herein, we explored association between expression,...
e15622 Background: Primary tumor sidedness (PTS) has been shown to be an independent prognostic factor for patients with metastatic colorectal cancer (CRC), worse prognosis right-sided tumors. There are limited data regarding the impact of PTS in stage III CRC. Objective: The main objective this study was analyze Methods: A retrospective and uni-institutional cohort performed a reference center São Paulo-SP, Brazil. All consecutive CRC treated 5-fluorouracil oxaliplatin-based chemotherapy...