A5064181231- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Toxin Mechanisms and Immunotoxins
- Infectious Diseases and Mycology
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Microbial Natural Products and Biosynthesis
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Trace Elements in Health
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Anorectal Disease Treatments and Outcomes
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Cancer Treatment and Pharmacology
- Pelvic floor disorders treatments
Peking Union Medical College Hospital
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2025
Chinese Academy of Agricultural Sciences
2020-2023
Institute of Vegetables and Flowers
2019-2023
Academy of Medical Sciences
2022
Agricultural Genomics Institute at Shenzhen
2021
Ministry of Agriculture and Rural Affairs
2021
Air Force Medical University
2020
Xijing Hospital
2020
Peking University
2013-2016
The ancient gymnosperm genus Taxus is the exclusive source of anticancer drug paclitaxel, yet no reference genome sequences are available for comprehensively elucidating paclitaxel biosynthesis pathway. We have completed a chromosome-level chinensis var. mairei with total length 10.23 gigabases. shared an ancestral whole-genome duplication coniferophyte lineage and underwent distinct transposon evolution. discovered unique physical functional grouping CYP725As (cytochrome P450) in...
The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating expression levels (COR) genes. In this study, we obtained CRISPR/Cas9-mediated loss-of-function mutants cbf1∼3. Over 3,000 COR genes identified RNA-seq analysis showed slight but significant change their compared wild-type plants after being treated at 4 °C for 12 h. C-repeat (CRT) motif (5'-CCGAC-3') was enriched promoters that were up-regulated CBF2...
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Cryptococcus neoformans poses a threat to human health, but anticryptococcal therapy is hampered by the emergence of drug resistance, whose underlying mechanisms remain poorly understood. Herein, we discovered that Isw1, an imitation switch chromatin remodeling ATPase, functions as master modulator genes responsible for in vivo and vitro multidrug resistance C. neoformans. Cells with disrupted ISW1 gene exhibited profound multiple antifungal drugs. Mass spectrometry analysis revealed Isw1...
Summary We used a monophyletic group of four natural populations Arabidopsis thaliana expanded from single ancestor along the Yangtze River c . 90 000 yr ago to study molecular mechanism divergence in their freezing tolerance, order gain an insight into genetic basis local adaption low temperatures. Freezing tolerance assays, measurements metabolites raffinose biosynthesis pathway and transactivation‐activity assays variation forms cold‐responsive transcription factors were conducted on...
Abstract The use of heterografts is widely applied for the production several important commercial crops, but molecular mechanism graft union formation remains poorly understood. Here, cucumber grafted onto pumpkin was used to study development, and genome-wide tempo-spatial gene expression at interface comprehensively investigated. Histological analysis suggested that resumption rootstock growth occurred after both phloem xylem reconnection, scion showed evident callus compared with 3 days...
Antifungal resistance poses a critical global health threat, particularly in immuno-compromised patients. Beyond the traditional mechanisms rooted heritable and stable mutations, distinct phenomenon known as heteroresistance has been concerned, wherein minority of resistant fungal cells coexist within predominantly susceptible population. It may be induced by pharmacological factors or non-pharmacological agents. The reversible nature it presents significant clinical challenges, can lead to...
Lysine acetylation is critical in regulating important biological processes many organisms, yet little known about acetylome evolution and its contribution to phenotypic diversity. Here, we compare the acetylomes of baker's yeast three deadliest human fungal pathogens,
Rootstocks frequently exert detrimental effects on the fruit quality of grafted cucumber (Cucumis sativus L.) plants. To understand and ultimately correct this deficiency, a transcriptomic metabolomic comparative analysis was performed among fruits from non-grafted plants (NG), onto different rootstocks No.96 No.45 (Cucurbita moschata. Duch), known to confer aroma taste. We found remarkable changes in primary metabolites sugars, organic acids, amino alcohols with rootstocks, compared ones,...
Mitochondrial quality control prevents accumulation of intramitochondrial-derived reactive oxygen species (mtROS), thereby protecting cells against DNA damage, genome instability, and programmed cell death. However, underlying mechanisms are incompletely understood, particularly in fungal species. Here, we show that Cryptococcus neoformans heat shock factor 3 (CnHsf3) exhibits an atypical function regulating mtROS independent the unfolded protein response. CnHsf3 acts nuclei mitochondria,...
Pathogens and hosts require rapid modulation of virulence defense mechanisms at the infection axis, but monitoring such modulations is challenging. In studying human fungal pathogen Cryptococcus neoformans, mouse rabbit models are often employed to shed light on disease that may not be clinically relevant. this study, we developed an animal model using non-human primate cynomolgus monkey Macaca fascicularis. addition, systematically profiled compared transcriptional responses between...
Tomato (Solanum lycopersicum) stress resistance and fruit total soluble solid (TSS) content have changed dramatically during selective breeding, transcriptome variation has played a critical role in this rewiring. However, the single tomato reference genome impedes characterization of whole-transcriptome domestication breeding at population level. Here, we constructed pan-transcriptome orange-stage fruit, investigated global expression presence/absence (ePAV) differentially expressed genes...
Early life is a critical window for preventing the intergenerational transmission of metabolic diseases. Betaine has been proven to play role in improving glucose and lipid metabolism disorders animal models. However, whether maternal betaine supplementation plays regulating gut microbiota both dams offspring remains unclear. In this study, C57BL/6 female mice were fed with control diet (Ctr), high-fat (HF), (0.3% diet, HFB) from 3 weeks prior mating lasted throughout pregnancy lactation....
ABSTRACT The underlying mechanism of thermotolerance, which is a key virulence factor essential for pathogenic fungi such as Cryptococcus neoformans , largely unexplored. In this study, our findings suggest that Set302, homolog Set3 and subunit histone deacetylase complex Set3C, contributes to thermotolerance in C. . Specifically, the deletion predicted Set3C core subunit, resulted further reduction growth at 39°C, survival transient incubation 50°C. Transcriptomics analysis revealed...
Hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP), coharboring hypervirulence and carbapenem-resistance genes mediated by plasmids, causes infections with extremely high mortality seriously impacts public health. Exploring the transfer mechanisms of virulence/carbapenem-resistance as well formation evolution pathway hv-CRKP is great significance to control infections.
Abstract Background The molecular and genetic mechanisms by which different single nucleotide variant alleles in specific genes, or at the same locus, cause distinct disease phenotypes often remain unclear. Allelic truncating mutations of FBN1 could either classical Marfan syndrome (MFS) a more complicated phenotype associated with Marfanoid–progeroid–lipodystrophy (MPLS). Methods We investigated small cohort, encompassing two MFS one MPLS subjects from China, whose clinical presentation...
Candida parapsilosis is an important species causing invasive candidiasis (IC) in China. The present survey was a national multicenter study of the molecular epidemiology and antifungal susceptibility profiles C. parapsilosis. Non-duplicate isolates were collected from 10 hospitals across China CHIF-NET program 2016-17. Isolates genotyped using four highly polymorphic microsatellite markers, determined Sensititre YeastOneTM YO10. A total 319 separate patients with IC studied; 49.2%, 17.9%,...
Abstract Objectives We explored the epidemiological and molecular characteristics of Candida parapsilosis sensu stricto isolates in China, their mechanisms azole resistance. Methods Azole susceptibilities 2318 non-duplicate were determined using CLSI broth microdilution. Isolates genotyped by a microsatellite typing method. Molecular resistance also studied functionally validated CRISPR/Cas9-based genetic alterations. Results Fluconazole occurred 2.4% (n = 56) isolates, these showed higher...
Invasive aspergillosis (IA) due to Aspergillus lentulus is associated with high mortality. In this study, we investigated the clinical and microbiological characteristics of 6 fatal cases proven or probable IA caused by A. in China. Underlying immunosuppression, prior antifungal exposure, intensive care unit (ICU) hospitalization were important risk factors for invasive infection. Phenotypic differences observed isolates including slower growth, reduced sporulation, inability grow at 48°C,...