A5016672355- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Renal and related cancers
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Wind Energy Research and Development
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Microtubule and mitosis dynamics
- Geophysics and Gravity Measurements
- Muscle Physiology and Disorders
- Genetic Neurodegenerative Diseases
- Plant Water Relations and Carbon Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Atmospheric chemistry and aerosols
- Dermatological and Skeletal Disorders
- Cell death mechanisms and regulation
- Genomics and Chromatin Dynamics
- Tree Root and Stability Studies
- Socio-economic Development and Sustainability
- Protein Tyrosine Phosphatases
- Peptidase Inhibition and Analysis
University of Debrecen
1999-2024
Albert Einstein College of Medicine
2011-2020
BioTalentum (Hungary)
2009-2018
Laboratoire de Biologie du Développement
2011
Medical University of Vienna
2010
University of Chicago
2006
Johns Hopkins Medicine
2004-2005
Johns Hopkins University
2004-2005
Aging is characterized by the accumulation of damaged cellular macromolecules caused declining repair and elimination pathways. An integral component employed cells to counter toxic protein aggregates conserved ubiquitin/proteasome system (UPS). Previous studies have described an age-dependent decline proteasomal function increased longevity correlates with sustained proteasome capacity in centenarians naked mole rats, a long-lived rodent. Proof for direct impact enhanced on longevity,...
Hepatocyte transplantation has the potential to cure inherited liver diseases, but its application is impeded by a scarcity of donor livers. Therefore, we explored whether hepatocyte-like cells (iHeps) differentiated from human induced pluripotent stem (iPSCs) could ameliorate diseases. iPSCs reprogrammed skin fibroblasts were iHeps, which transplanted into livers uridinediphosphoglucuronate glucuronosyltransferase-1 (UGT1A1)-deficient Gunn rats, model Crigler-Najjar syndrome 1 (CN1), where...
Abstract Our recently published data suggested the involvement of protein phosphatase 2A (PP2A) in endothelial cell (EC) barrier regulation (Tar et al. [2004] J Cell Biochem 92:534–546). In order to further elucidate role PP2A EC cytoskeleton and permeability, catalytic (PP2Ac) A regulatory (PP2Aa) subunits were cloned human pulmonary arterial (HPAEC) transfected with mammalian expression constructs or infected recombinant adenoviruses. Immunostaining PP2Ac PP2Aa + c overexpressing HPAEC...
Abstract Background The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in regulation of pluripotency during embryonic development and widely used as molecular marker stem cells (ESCs). objective this study was to identify analyse promoter region rabbit gene; furthermore examine its expression pattern preimplantation stage embryos. Results upstream subcloned sequenced four highly conserved regions (CR1-4) were identified. highest degree similarity on...
The regulation of ribosomal protein (RP) gene transcription is tightly linked to the nutrient status cell and under control metabolic signaling pathways. In Saccharomyces cerevisiae several transcriptional activators mediate efficient RP during logarithmic growth dissociate from promoters upon limitation. Repression appears be regulated predominantly by posttranslational modification cellular localization activators. We report here that one these factors, Sfp1, degraded proteasome activator...
Elevated proteasome activity extends lifespan in model organisms such as yeast, worms and flies. This pro-longevity effect might be mediated by improved protein homeostasis, this protease is an integral module of the homeostasis network. Proteasomes also regulate cellular processes through temporal spatial degradation signaling pathway components. Here we demonstrate that regulatory function plays essential role aging cells beneficial impact elevated capacity on partially originates from...
Background: Dynamin-related protein Drp1 —a major mitochondrial fission protein— is widely distributed in the central nervous system and plays a crucial role regulating dynamics, specifically organelle's shaping. Upregulated function may contribute to pathological progression of neurodegenerative diseases by dysregulating fission/ fusion. The study aims investigate effects on retinoic acid-BDNF-induced (RA-BDNF) neuronal differentiation network reorganization SH-SY5Y neuroblastoma cells....
All-trans retinoic acid (ATRA) is a derivative of vitamin A that has many important biological functions, including the modulation immune responses. ATRA actions are mediated through receptor functions as nuclear receptor, either regulating gene transcription in nucleus or modulating signal transduction cytoplasm. NLRP3 inflammasome multiprotein complex activated by huge variety stimuli, pathogen- danger-related molecules. Activation required for production IL-1β, which drives inflammatory...
Although several types of somatic cells have been reprogrammed into induced pluripotent stem (iPSCs) and then differentiated to hepatocyte-like (iHeps), the method for generating such from renal tubular epithelial shed in human urine transplanting them animal livers has not described systematically. We report reprogramming urinary iPSCs subsequent hepatic differentiation, followed by a detailed characterization newly generated iHeps. The were delivering pluripotency factors OCT3/4, SOX2,...
Abstract We have recently shown that microtubule (MT) inhibitor, nocodazole (2–5 μM) significantly increases endothelial cells (EC) actomyosin contraction and permeability indicating the importance of MT in maintaining EC barrier (Verin et al. [ 2001 ]: Cell Mol Physiol 281:L565–L574). Okadaic acid (OA, 2–5 nM), a powerful inhibitor protein phosphatase 2A (PP2A), potentiates effect submaximal concentrations (50–200 nM) on transendothelial electrical resistance (TER) suggesting involvement...
Huntington's disease (HD) is an inherited neurodegenerative disorder, caused by abnormal polyglutamine (polyQ) expansion in the huntingtin protein (Htt). Mitochondrial dysfunction and impairment of ubiquitin-proteasome system (UPS) are hallmarks HD neurons. The extraneural manifestations still unclear. We investigated crosstalk between mitochondria proteolytic function skin fibroblasts from juvenile patients. found reduced mitosis, increased cell size, elevated ROS mitochondrial membrane...
Continuous fusion and fission are critical for mitochondrial health. In this study, we further characterize the role played by dynamin‐related protein 1 (Drp1) in fission. We show that a single amino acid change Drp1 at position 39 from serine to alanine (S39A) within GTP‐binding (GTPase) domain results fused network human SH‐SY5Y neuroblastoma cells. Interestingly, phosphorylation of Ser‐616 Ser‐637 remains unaffected S39A mutation, bioenergetic profile cell viability mutant were comparable...
Magmas is a growth factor responsive gene encoding an essential mitochondrial protein in mammalian cells. Pam16, the ortholog Saccharomyces cerevisiae, component of presequence translocase-associated motor. A temperature-sensitive allele (pam16-I61N) was used to query array non-essential gene-deletion strains for synthetic genetic interactions. The pam16-I61N mutation at ambient temperature caused lethal or sick phenotypes with genes involved lipid metabolism, perixosome synthesis, histone...
The Blm10/PA200 family of proteasome activators modulates the peptidase activity core particle (20S CP). They participate in opening 20S CP gate, thus facilitating degradation unstructured proteins such as tau and Dnm1 a ubiquitin- ATP-independent manner. Furthermore, PA200 also participates acetylated histones. In our study, we use combination yeast human cell systems to investigate role N-terminal Huntingtin fragments (N-Htt). We demonstrate that binds N-Htt. loss Blm10 or cells results...
Changes to the spatial organization of specific chromatin domains such as constitutive heterochromatin have been studied extensively in somatic cells. During early embryonic development, drastic epigenetic reprogramming both maternal and paternal genomes, followed by remodeling at time genome activation (EGA), observed mouse. Very few studies performed other mammalian species (human, bovine, or rabbit) data are far from complete. this work, we three-dimensional pericentromeric regions during...
The conserved Blm10/PA200 activators bind to the proteasome core and facilitate peptide protein turnover. proteins enhance peptidase activity accelerate degradation of unstructured substrates. Our knowledge about exact role PA200 in diseased cells, however, is still limited. Here, we show that stable knockdown leads a significantly elevated number cells S phase after treatment with ATP synthase inhibitor, oligomycin. However, following exposure complex I inhibitor rotenone, more...
SYNOPSIS In this study changes in the surface pressure field over Europe are analysed to see if they reflected statistical structure of wind Great Hungarian Plain. The data bais consists hourly speed three meteorological stations (Debrecen, Békéscsaba and Szeged) at Plain, coming from periods between 1968–72 1991–95, respectively. A new test is also shown paper, order apply it for determining significance differences expected values non-independent time series. Decrease energy might be...