A5041661432- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Biomedical Text Mining and Ontologies
- Catalytic Processes in Materials Science
- GABA and Rice Research
- Semantic Web and Ontologies
- DNA and Nucleic Acid Chemistry
- Advanced Nanomaterials in Catalysis
- Anesthesia and Neurotoxicity Research
- Coal and Its By-products
- Insect-Plant Interactions and Control
- Air Quality Monitoring and Forecasting
- Thermochemical Biomass Conversion Processes
- Quantum Dots Synthesis And Properties
- Plant tissue culture and regeneration
- Anesthesia and Sedative Agents
- Natural Language Processing Techniques
- RNA Interference and Gene Delivery
- Plant Parasitism and Resistance
- Nanocluster Synthesis and Applications
- Water Quality Monitoring and Analysis
- Biometric Identification and Security
- MicroRNA in disease regulation
- Smart Parking Systems Research
China Pharmaceutical University
2017-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2024
Guangzhou University of Chinese Medicine
2023
Shanxi Medical University
2022-2023
South China University of Technology
2023
North University of China
2023
Second Hospital of Shanxi Medical University
2022-2023
Huazhong University of Science and Technology
2023
First Affiliated Hospital of Guangdong Pharmaceutical University
2021-2022
China Special Equipment Inspection and Research Institute
2022
A sensitive chemiluminescence (CL) imaging immunoassay method for detection of multiple tumor markers with high throughput, easy operation, and low cost was developed. The immunosensor array prepared by covalently immobilizing capture antibodies on corresponding sensing sites a silanized disposable glass chip. Gold nanoparticle-based bioconjugates molar ratio horseradish peroxidase (HRP) to were used signal amplification. Under sandwich immunoassay, the CL signals triggered HRP captured each...
Abstract It is reported that overweight may lead to accelerated aging. However, there still a lack of evidence on the causal effect and We collected genetic variants associated with overweight, age proxy indicators (telomere length, frailty index facial aging), etc., from genome‐wide association studies datasets. Then we performed MR analyses explore associations between indicators. were primarily conducted using inverse variance weighted method, followed by various sensitivity validation...
Manganese porphyrin (MnTMPyP)-dsDNA complex was reported as an excellent mimicking enzyme of peroxidase. It possessed high catalytic activity and much quicker kinetics better stability with exposure to light irradiation temperature than both horseradish peroxidase hemin/G-quadruplex DNAzyme. The groove binding MnTMPyP the dsDNA scaffold efficiently maintained center improved its stability. By combining isothermal hybridization chain reaction (HCR) in situ formation MnTMPyP-dsDNA, a highly...
An array-based chemiluminescence (CL) imaging method is presented for simple and high throughput detection of protein targets via the formation a proximity-dependent DNAzyme to produce sensitive CL signal. The array prepared by covalently immobilizing single-stranded guanine-rich nucleic acid 1-labeled antibody 1 (GDNA1-Ab1) or GDNA-thrombin aptamer subunit (Apt-P1) as capture probe on each sensing site an aldehyde-functionalized disposable glass chip. In presence target protein, hemin,...
An automated support-resolution strategy was designed to couple with a flow-through immunosensing system for performing one-way chemiluminescent (CL) multiplex immunoassay. Different from multilabel and multichannel-based detection techniques, this immunoassay method employed single horseradish peroxidase (HRP) label in one way. With the use of carcinoembryonic antigen (CEA) alpha-fetoprotein (AFP) as model analytes, capture antibodies CEA AFP were immobilized on inner wall glass tube...
A proximity hybridization-triggered signal switch was presented for convenient homogeneous chemiluminescent detection of a wide range affinity target biomolecules, such as oligonucleotides, protein biomarkers, and aptamer-recognized targets. The presence the promoted formation proximate complex via hybridization two help DNA strands or labeled to ligands, which subsequently unfolded self-reporting molecular beacon on chemiluminescence signal. response could be further amplified with an in...
Chimeric antigen receptor-T (CAR-T) cell therapy is a cellular immunotherapy that has emerged in recent years, and increasing studies showed therapeutic resistance to CAR-T presents colorectal cancer patients. Lipopolysaccharide (LPS), component of the wall Gram-negative bacteria, known preserve high concentration colon. Whether LPS contributing factor development cells against remains unclear. For vivo experiments, COLO205 were pretreated with for 24 h then injected into nude mice through...
A versatile imaging strategy integrated with target-induced DNA assembly and cleavage was designed for an assay multiple proteins.
Water-soluble doped quantum dots have unique photophysical properties and functionalities as optical labels for bioimaging chemo-/biosensing. However, doping in is not easy due to the dopant-ion size mismatch "self-purification" effect. Here, we demonstrate a successful preparation of Mn-, Cu-, Ni-doped CdS with bimetallic clusters instead ions building blocks under mild aqueous conditions up gram scale. The representative Mn-doped uniform size, about 3.2 ± 0.5 nm, emit at 620 nm....
A multiplex assay of mycotoxins in food and medicine is urgently needed challenging due to synergistic hazards trace a lack sensitive user-friendly detection approaches. Herein, cobalt DNA-inorganic hybrid superstructure (Co@DS) was developed through isothermal rolling circle amplification (RCA) for an ultrasensitive chemiluminescence (CL) imaging multiple mycotoxins. Cobalt ions were enriched the RCA product, endowing Co@DS with high CL catalytic property. Experimental studies elucidated...
A novel trace tag for chemiluminescent (CL) immunoassay was designed by using DNAzyme to functionalize antibody-labeled Au nanoparticles (AuNPs). The showed an excellent ability catalyze the oxidation of luminol hydrogen peroxide, leading strong CL emission. By coupling with a passive mixing accelerated immunoreaction system, highly sensitive rapid flow-through method proposed. Using carcinoembryonic antigen (CEA) as model analyte, capture antibody CEA immobilized on paramagnetic...
Efficient apoptosis requires Bcl-2 family-mediated mitochondrial outer membrane permeabilization (MOMP), which releases pro‑apoptotic proteins to the cytosol, activating and inhibiting X‑linked inhibitor of protein (XIAP). XIAP is a member inhibitors family whose expression elevated in many cancer types participates release proteins. To explore association between Bcl‑2 family, influence on mitochondria, RNA interference was performed Caki‑1 cells dynamic change levels related compared with...