A5083078779- Advanced Fluorescence Microscopy Techniques
- Glycogen Storage Diseases and Myoclonus
- Pancreatic function and diabetes
- Photoacoustic and Ultrasonic Imaging
- Advanced Electron Microscopy Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Electromagnetic Fields and Biological Effects
- Bacteriophages and microbial interactions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Diabetes and associated disorders
- Cell Image Analysis Techniques
- Lanthanide and Transition Metal Complexes
- Dialysis and Renal Disease Management
- Reproductive Biology and Fertility
- Plant Pathogens and Fungal Diseases
- vaccines and immunoinformatics approaches
- Lysosomal Storage Disorders Research
- COVID-19 epidemiological studies
- Renal and related cancers
- PARP inhibition in cancer therapy
- SARS-CoV-2 detection and testing
- Single-cell and spatial transcriptomics
- Drug Transport and Resistance Mechanisms
- Genetics and Neurodevelopmental Disorders
- Renal and Vascular Pathologies
Institute of Molecular Biology and Pathology
2024
IFOM
2024
National Research Council
2024
University of Milan
2024
Biogem
2018-2023
University of Bologna
2018-2023
Istituto Giannina Gaslini
2022
Ospedale Policlinico San Martino
2022
Aarhus University
2022
SARS-CoV-2, one of the human RNA viruses, is widely studied around world. Significant efforts have been made to understand its molecular mechanisms action and how it interacts with epithelial cells microbiome since has also observed in gut bacteria. Many studies emphasize importance surface immunity that mucosal system critical interaction pathogen oral, nasal, pharyngeal, intestinal epithelium. Recent shown bacteria produce toxins capable altering classical viruses cells. This paper...
The COVID-19 pandemic has sparked a surge in research on microbiology and virology, shedding light overlooked aspects such as the infection of bacteria by RNA virions animal microbiome. Studies reveal decrease beneficial gut during COVID-19, indicating significant interaction between SARS-CoV-2 human However, determining origins virus remains complex, with observed phenomena species jumps adding layers to narrative. Prokaryotic cells play crucial role disease’s pathogenesis transmission....
Glycogen storage disease XI, also known as Fanconi-Bickel syndrome (FBS), is a rare autosomal recessive disorder caused by mutations in the SLC2A2 gene that encodes glucose-facilitated transporter type 2 (GLUT2). Patients develop life-threatening renal proximal tubule dysfunction for which no treatment available apart from electrolyte replacement. To investigate pathogenesis of FBS, expression was ablated mouse kidney and HK-2 cells. GLUT2Pax8Cre+ mice developed time-dependent glycogen...
MicroRNAs (miRNAs), formed by cleavage of pre-microRNA the endoribonuclease Dicer, are critical modulators cell function post-transcriptionally regulating gene expression.Selective ablation Dicer in AQP2-expressing cells (DicerAQP2Cre+ mice) was used to investigate role miRNAs kidney collecting duct mice.The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due greatly reduced AQP2 AQP4 levels. Although epithelial sodium channel levels were decreased cortex increased...
Abstract Renal micropuncture, which requires the direct access to renal tubules, has for long time been technique of choice measure single nephron glomerular filtration rate (SNGFR) in animal models. This approach is challenging by virtue complex preparation and numerous technically difficult steps. The introduction intravital multiphoton microscopy (MPM) offers another SNGFR mean high laser-tissue penetration optical sectioning capacity. Previous MPM studies measuring vivo relied on fast...
Mutations in SLC37A4, which encodes the intracellular glucose transporter G6PT, cause rare glycogen storage disease type 1b (GSD1b). A long-term consequence of GSD1b is kidney failure, requires KRT. The main protein markers proximal tubule function, including NaPi2A, NHE3, SGLT2, GLUT2, and AQP1, are downregulated as part phenotype.We utilized an inducible mouse model GSD1b, TM-G6PT-/-, to show that accumulation plays a crucial role altering morphology function. To limit entry into cells...
Accumulation of uremic toxins may lead to the life-threatening condition “uremic syndrome” in patients with advanced chronic kidney disease (CKD) requiring renal replacement therapy. Clinical evaluation proximal tubular secretion organic cations (OC), which some are toxins, is desired, but difficult. The biomedical knowledge on OC and cellular transport partly relies studies using fluorescent tracer 4-dimethylaminostyryl)- N-methylpyridinium (ASP+), has been used many excretion mechanisms...
Future MicrobiologyVol. 17, No. 12 CommentaryTree-inhabiting polypore fungi as sources of a cornucopia bioactive compoundsVincenzo Costanzo‡, Melinda Gilhen-Baker‡, Diana Beresford-Kroeger & Giovanni N RovielloVincenzo Costanzo‡ https://orcid.org/0000-0002-1995-7991Department Experimental, Diagnostic Specialty Medicine, University Bologna Alma Mater Studiorum, Via Zamboni 33, Bologna, 40126, Italy, Gilhen-Baker‡ https://orcid.org/0000-0003-4133-9978Faculty Physical Medicine Rehabilitation,...
Fluorescence microscopy has represented a crucial technique to explore the cellular and molecular mechanisms in field of biomedicine. However, conventional one-photon exhibits many limitations when living samples are imaged. The new technologies, including two-photon (2PM), have considerably improved vivo study pathophysiological processes, allowing investigators overcome limits displayed by previous techniques. 2PM enables real-time intravital imaging biological functions different organs...
A total of 52 paleomagnetists, rock magnetists, and structural geologists met for the 5th annual Northeastern Paleomagnetic Rock Magnetic Workshop, held at Erindale College, University Toronto in Mississauga, Ontario, on November 1–2. The attendees came from as far away Lamont‐Doherty Geological Observatory, N.Y.; Massachusetts, Amherst; Lehigh University, Bethlehem, Pa.; Memorial St. John, Newfoundland; well Michigan, Ann Arbor; Windsor, Ontario; Sapphire Instruments, Ruthven, Western...
Abstract BACKGROUND AND AIMS Peritoneal dialysis (PD) is a renal replacement therapy that allows the elimination of metabolic waste products and excess body fluid through peritoneal membrane. The exposure to PD solution contributes membrane aging fibrosis resulting in ultrafiltration clearance failure. glucose, used as an osmotic factor, dialysate triggers several processes involved pathogenesis fibrosis, angiogenesis epithelial mesenchymal transition. Studies on natural extracts, such...
In the original publication [...]
Abstract Background and Aims Renal micropuncture, which requires the direct access to renal tubules, has been for long time technique of choice measure single nephron glomerular filtration rate (SNGFR) in animal models, but this approach is challenging by virtue complex preparation numerous careful steps. The introduction intravital multiphoton microscopy (MPM) permitted improve study functions exploiting high laser penetration optical sectioning capacity. Previous MPM studies measuring vivo...