A5100744970- Ion Transport and Channel Regulation
- Plant tissue culture and regeneration
- Gastric Cancer Management and Outcomes
- Cancer-related molecular mechanisms research
- Hormonal Regulation and Hypertension
- Animal Genetics and Reproduction
- Sodium Intake and Health
- Reproductive Biology and Fertility
- Reproductive Health and Contraception
- Esophageal Cancer Research and Treatment
- Electrolyte and hormonal disorders
- Metastasis and carcinoma case studies
- MicroRNA in disease regulation
- Renal and related cancers
- Advanced Proteomics Techniques and Applications
- Esophageal and GI Pathology
- Pancreatic function and diabetes
- Bladder and Urothelial Cancer Treatments
- Birth, Development, and Health
- Gastrointestinal Tumor Research and Treatment
- Circular RNAs in diseases
- Quantum Chromodynamics and Particle Interactions
- S100 Proteins and Annexins
- Extracellular vesicles in disease
- Colorectal Cancer Surgical Treatments
Nankai University
2024
Zhejiang Provincial People's Hospital
2024
Aarhus University
2014-2023
Xinjiang Medical University
2013-2023
Tumor Hospital of Xinjiang Medical University
2013-2023
Nanjing Institute of Agricultural Mechanization
2023
Ministry of Agriculture and Rural Affairs
2023
Zhejiang Cancer Hospital
2014-2021
University of Chinese Academy of Sciences
2017-2021
Cancer Hospital of Chinese Academy of Medical Sciences
2021
BACKGROUND: LncRNA and microRNA play an important role in the development of human cancers; they can act as a tumor suppressor gene or oncogene. GAS5, originating from separation cDNA subtractive library, is c
Colorectal cancer (CRC) is a frequently occurring lethal disorder with heterogeneous outcomes and drug responses. Recent studies have demonstrated that long non-coding RNAs (lncRNAs) play critical role in carcinogenesis. Hence, the aim of this study was to investigate lncRNA growth arrest-specific 5 (GAS5) CRC cells via mediation microRNA-222-3p (miR-222-3p)/GAS5/phosphatase tensin homolog (PTEN)-signaling pathway. HCT116 SW480 were collected treated small interfering (si)-lncRNA GAS5,...
<h3>Importance</h3> Most older patients with esophageal cancer cannot complete the standard concurrent chemoradiotherapy (CCRT). An effective and tolerable regimen for is needed. <h3>Objective</h3> To evaluate efficacy toxic effects of CCRT S-1 vs radiotherapy (RT) alone in cancer. <h3>Design, Setting, Participants</h3> A randomized, open-label, phase 3 clinical trial was conducted at 23 Chinese centers between June 1, 2016, August 31, 2018. The study enrolled 298 aged 70 to 85 years....
Immobilized metal ion affinity chromatography (IMAC) is widely used for phosphopeptide enrichment. However, the robustness, efficiency, and specificity of this technique in large-scale phosphoproteomics studies are still disputed. In study, we first compared three IMAC materials under different conditions. Fe(III)-nitrilotriacetic acid (NTA) resin was chosen due to its superior performance all tests. We further investigated solution ionization efficiency change phosphoryl group carboxylic...
Dietary potassium (K+) supplementation is associated with a lowering effect in blood pressure (BP), but not all studies agree. Here, we examined the effects of short- and long-term K+ on BP mice, whether differences depend accompanying anion or sodium (Na+) intake molecular alterations kidney that may underlie changes. Relative to control diet, was higher mice fed high NaCl (1.57% Na+) diet for 7 weeks K+-free 2 weeks. highest K+-free/high diet. Commensurate increased abundance...
BACKGROUND: Mifepristone in low daily doses has contraceptive potential by inhibiting ovulation and menstruation. Because follicular development is maintained, the endometrium exposed to estrogen for prolonged periods unopposed progesterone. METHODS: Endometrial biopsies were collected from 90 women Edinburgh Shanghai before (late proliferative) 60 120 days after taking 2 or 5 mg mifepristone per day days. RESULTS: Ovulation menstruation inhibited >90% of cycles production was similar that...
Enteroendocrine (EEC) cells have a pivotal role in intestinal nutrient sensing and release hormones that orchestrate food digestion regulate appetite. EEC are found scattered throughout the intestine typically been classified based on primary hormone they contain. I represent subset of secrete cholecystokinin (CCK) mainly localized to duodenum. Recent studies shown express mRNAs encoding several gut hormones. In this study, we investigated hormonal profile murine fluorescence-activated cell...
Exosomes, derived from multivesicular bodies (MVBs), contain proteins and genetic materials their cell of origin are secreted various cells types, including kidney epithelial cells. In general, it is thought that protein cargo ubiquitylated but ubiquitin cleaved by specific deubiquitylases during the process incorporation into MVBs. Here, we provide direct evidence that, in vivo, deubiquitylation not essential. Ubiquitin was detected within human MVBs urinary exosomes electron microscopy. Of...
Significance Statement The NaCl cotransporter NCC in the kidney distal convoluted tubule (DCT) regulates urinary excretion and BP. long-term effects of mineralocorticoid aldosterone on modulating reabsorption via are well established, their importance illustrated by NCC-targeting diuretics salt-wasting observed Gitelman syndrome. In this study authors demonstrate that also has rapid DCT, partly membrane receptors EGFR GPR30. Signaling from these affect activity so when is released response...
The importance of the kidney distal convoluted tubule (DCT) and cortical collecting duct (CCD) is highlighted by various water electrolyte disorders that arise when unique transport properties these segments are disturbed. Despite this critical role, little known about which proteins have a regulatory role in cells how can be regulated individual physiologic stimuli. By combining proteomics, bioinformatics, cell biology approaches, we found E3 ubiquitin ligase CHIP highly expressed...
The putative "renal-K switch" mechanism links dietary potassium intake with sodium retention and involves activation of the chloride (NaCl) cotransporter (NCC) in distal convoluted tubule response to low intake, suppression high intake. This study examined NCC abundance phosphorylation (phosphorylated [pNCC]) urinary extracellular vesicles (uEVs) isolated from healthy adults on a diet determine tubular responses alteration (KCl) intake.Healthy maintained (∼4.5 g [200 mmol]/d) (∼2.3 [60...
It has been suggested that the administration of an anti-progesterone might improve bleeding patterns in women with irregular while using low-dose progestin-only contraception. We report findings a double-blind, randomized, placebo-controlled trial mifepristone 50 mg taken once every 4 weeks 100 Chinese (50 subjects and controls) complaining frequent levonorgestrel-releasing subdermal contraceptive implant. In all women, regardless treatment, frequency decreased significantly over 360 days...
// Yongchun Zuo 1, 2, * , Guanghua Su Lei Cheng 1 Kun Liu Yu Feng Zhuying Wei Chunling Bai Guifang Cao 2 and Guangpeng Li The Research Center for Laboratory Animal Science, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China Veterinary Medicine, Agricultural 010018, These authors have contributed equally to this work Correspondence to: Zuo, email: yczuo@imu.edu.cn Li, gpengli@imu.edu.cn Keywords: somatic cell nuclear transfer (SCNT), gene co-expression analysis,...
Abstract The kidney distal convoluted tubule (DCT) plays an essential role in maintaining body sodium balance and blood pressure. major reabsorption pathway the DCT is thiazide-sensitive NaCl cotransporter (NCC), whose functions can be modulated by hormone vasopressin (VP) acting via uncharacterized signaling cascades. Here we use a systems biology approach centered on stable isotope labeling amino acids cell culture (SILAC) based quantitative phosphoproteomics of cultured mouse cells to map...
MicroRNAs (miRNAs), formed by cleavage of pre-microRNA the endoribonuclease Dicer, are critical modulators cell function post-transcriptionally regulating gene expression.Selective ablation Dicer in AQP2-expressing cells (DicerAQP2Cre+ mice) was used to investigate role miRNAs kidney collecting duct mice.The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due greatly reduced AQP2 AQP4 levels. Although epithelial sodium channel levels were decreased cortex increased...
The increasing use of emergency contraceptive pills (ECPs) does not seem to reduce the number induced abortions as would be expected, indicating that women ECPs might also a strong factor affecting their final efficacy. study aimed understand attitude towards, and of, among seeking an abortion. A cohort was conducted via face-to-face questionnaire interview abortion in Shanghai, China. Logistic regression analysis χ2 test were performed for statistical analysis. response rate 99.3%. Among...
Background Although it is known that RNA interference (RNAi) targeting viral genes protects experimental animals, such as mice, from the challenge of Foot-and-mouth disease virus (FMDV), has not been previously investigated whether shRNAs FMDV in transgenic dairy cattle or primary bovine epithelium cells will confer resistance against challenge. Principal Finding Here we constructed three recombinant lentiviral vectors containing shRNA VP2 (RNAi-VP2), VP3 (RNAi-VP3), VP4 (RNAi-VP4) FMDV, and...
Aquaporin-2 (AQP2) is essential to maintain body water homeostasis. AQP2 traffics from intracellular vesicles the apical membrane of kidney collecting duct principal cells in response vasopressin [arginine (AVP)], a hormone released with low intravascular volume, which causes decreased perfusion. Decreased perfusion activates AMP-activated kinase (AMPK), metabolic sensor that inhibits activity several transport proteins. We hypothesized AMPK activation also function. These putative effects...
(5R)-5-hydroxytriptolide (LLDT-8), a triptolide derivative with low toxicity, was previously reported to have strong immunosuppressive effects both in vitro and vivo, but it remains unknown whether LLDT-8 has therapy effect on systemic lupus erythematosus. In this study, we aimed investigate the therapeutic of nephritis MRL/ lpr mice, model Compared vehicle group, different clinical parameters were improved upon treatment as follows: prolonged life span decreased proteinuria, downregulated...