A5034294664- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- Biopolymer Synthesis and Applications
- Diet and metabolism studies
- Photoreceptor and optogenetics research
- Metabolomics and Mass Spectrometry Studies
- Pluripotent Stem Cells Research
- Biotin and Related Studies
- Signaling Pathways in Disease
- Genetic Neurodegenerative Diseases
- Dementia and Cognitive Impairment Research
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- bioluminescence and chemiluminescence research
- COVID-19 Clinical Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- ATP Synthase and ATPases Research
- Amino Acid Enzymes and Metabolism
- Genomics and Rare Diseases
- Autophagy in Disease and Therapy
- Chromatin Remodeling and Cancer
- Advanced Biosensing Techniques and Applications
- Mosquito-borne diseases and control
- Ubiquitin and proteasome pathways
First Affiliated Hospital of Zhengzhou University
2018-2025
Academy of Medical Sciences
2025
Zhengzhou University
2022-2025
Phoenix Contact (United States)
2024
Chinese Academy of Sciences
2001-2018
University of Chinese Academy of Sciences
2016-2018
Shanghai Institutes for Biological Sciences
2001-2018
Seoul National University Dental Hospital
2018
National Institutes of Health
2008
National Institute of Neurological Disorders and Stroke
2008
A fundamental question in synaptic physiology is whether the unitary strength of a synapse can be regulated by presynaptic characteristics and, if so, what those might be. Here, we characterize newly proposed mechanism for altering glutamatergic synapses based on recently identified vesicular glutamate transporter VGLUT1. We provide direct evidence that filling isolated vesicles subject to dynamic equilibrium determined both concentration available and number transporters participating...
Existing techniques have many limitations in the diagnosis and classification of ischemic stroke (IS). Considering this, we used metabolomics to screen for potential biomarkers IS its subtypes explore underlying related pathophysiological mechanisms. Serum samples from 99 patients with acute (AIS) [the AIS included 49 large artery atherosclerosis (LAA) 50 small occlusion (SAO)] matched healthy controls (HCs) were analyzed by non-targeted based on liquid chromatography–mass spectrometry. A...
Abstract Aging is an intricate process involving interactions among multiple factors, which one of the main risks for chronic diseases, including Alzheimer's disease (AD). As a member cysteine protease, cathepsin S (CTSS) has been implicated in inflammation across various diseases. Here, we investigated role neuronal CTSS aging and AD started by examining expression hippocampus neurons mice identified significant increase, was negatively correlated with recognition abilities. Concurrently,...
Mitochondrial membrane potential (MMP, ΔΨm) is critical for mitochondrial functions, including ATP synthesis, ion transport, reactive oxygen species (ROS) generation, and the import of proteins encoded by nucleus. Existing methods measuring ΔΨm typically use lipophilic cation dyes, such as Rhodamine 800 tetramethylrhodamine methyl ester (TMRM), but these are limited low specificity not well-suited in vivo applications. To address limitations, we have developed a novel protocol utilizing...
STX12 (syntaxin12 or syntaxin13), a member of the SNARE protein family, plays crucial role in intracellular vesicle transport and membrane fusion. Our previous research demonstrated that Stx12 knockout mice exhibit perinatal lethality with iron deficiency anemia. Despite its importance, comprehensive physiological pathological mechanism remains largely unknown. Here, we revealed causes depolarization mitochondrial potential zebrafish embryos mouse embryonic fibroblasts. Additionally, loss...
Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as thermosensitive mixture nonfluorescent fluorescent resonance forms. Using this approach, are able to demonstrate that efficacy norepinephrine-induced low, measure maximum transient rate temperature increase in brown adipocytes.The online version article...
Calcium influx associated with the opening of N-methyl-d-aspartate (NMDA) receptor channels is major signal triggering synaptic and developmental plasticity. Controlling NMDA function therefore critical for many functions brain. We explored mechanisms activation NMDAR glycine site by endogenous coagonist using whole cell voltage-clamp recordings from hippocampal neurons in mixed cultures, containing both glial cells, and, under more physiological conditions, slices. Here we show that at...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Abstract Obesity is a worldwide epidemic and results from excessive energy intake or inefficient expenditure. It promising to utilize the thermogenic function of brown adipose tissue for obesity intervention. However, mechanisms controlling efficacy norepinephrine-induced thermogenesis in adipocytes remain elusive. Here we demonstrate that norepinephrine (NE) induces low-efficacy thermogenesis, evoking both heterogeneous changes (ΔΨ m ΔpH) homogenous responses, one which NE stimulation...
Neuronal atrophy is a common pathological feature occurred in aging and neurodegenerative diseases. A variety of abnormalities including motor protein malfunction mitochondrial dysfunction contribute to the loss neuronal architecture; however, less known about intracellular signaling pathways that can protect against or delay this pathogenic process. Here, we show DYNC1I1 deficiency, neuron-specific dynein intermediate chain, causes primary hippocampal neurons. With cellular model, are able...
Abstract Parkinson's disease (PD) is a mitochondria‐related neurodegenerative characterized by locomotor deficits and loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc). Majority PD research primarily focused on neuronal dysfunction, while roles astrocytes their mitochondria remain largely unexplored. To bridge gap investigate astrocytic progression, we constructed specialized optogenetic tool, mitochondrial‐targeted anion channelrhodopsin, to manipulate...
Cytoplasmic dynein is an important intracellular motor protein that plays role in neuronal growth, axonal polarity formation, dendritic differentiation, and spine development among others. The intermediate chain of dynein, encoded by Dync1i1, a vital the complex. Therefore, we assessed behavioral related activities mice with dync1i1 gene knockout. Neuronal primary somatosensory cortex were recorded vivo electrophysiology manipulated optogenetic chemogenetics. Nociception mechanical, thermal,...
Abstract Ciliary neurotrophic factor receptor α subunit (CNTFRα) and CNTF play important roles in neuron survival, glial differentiation brain tumor growth. However, the molecular mechanisms of CNTFRα regulation its clinical significance glioma remain largely unknown. Here, we found was overexpressed lower grade gliomas (LGG) compared with glioblastoma (GBM) normal specimens TCGA datasets an independent cohort. Bioinformatics analysis revealed a CpG shore gene regulated mRNA expression...
Temperature distributions inside a living cell reflect the thermodynamics and functions of cellular components. We used newly-developed method mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as thermosensitive mixture nonfluorescent fluorescent resonance forms. Prostaglandin E2 (PGE2) is released from hepatic non-parenchymal Kupffer cells acts an inflammatory factor to impact various hepatocytes. The activity PGE2 energy mechanism hepatocytes has not been...
Based on the first law of thermodynamics and thermal diffusion equation, deduced theoretical model mitochondrial thermogenesis satisfies Laplace equation is a special case equation. The settles long-standing question ability to increase cellular temperature by endogenous explains thermogenic characteristics brown adipocytes. calculations also suggest that number free available protons major limiting factor for its speed.
The temperature of a living cell is crucial parameter for cellular events, such as division, gene expressions, enzyme activities and metabolism. We previously developed quantifiable mitochondrial thermometry 1.0 based on rhodamine B methyl ester (RhB-ME) 800 (Rh800), the theory thermogenesis. Given that synthesized RhB-ME not readily available, thus, convenient 2.0 tetra-methyl (TMRM) Rh800 thermogenic study brown adipocyte was further evolved. fluorescence TMRM more sensitive (∼1.4 times)...