A5006469627- Eosinophilic Esophagitis
- Eosinophilic Disorders and Syndromes
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Trypanosoma species research and implications
- MicroRNA in disease regulation
- Renewable Energy and Sustainability
- Food Allergy and Anaphylaxis Research
- Regulation of Appetite and Obesity
- Circadian rhythm and melatonin
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Allergic Rhinitis and Sensitization
- Neurobiology and Insect Physiology Research
- Adipokines, Inflammation, and Metabolic Diseases
- Toxin Mechanisms and Immunotoxins
- Esophageal and GI Pathology
- Mast cells and histamine
- Studies on Chitinases and Chitosanases
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Sleep and Wakefulness Research
- Neonatal Respiratory Health Research
Cincinnati Children's Hospital Medical Center
2009-2022
University of Cincinnati Medical Center
2022
University of Cincinnati
2010-2018
Boston Children's Hospital
2009
Michigan State University
2004-2006
An altered balance between Th1 and Th2 cytokines is responsible for a variety of immunoinflammatory disorders such as asthma, yet the role posttranscriptional mechanisms, those mediated by microRNAs (miRs), in adjusting relative magnitude Th cytokine expression have been largely unexplored. In this study, we show that miR-21 has central setting responses to Ags. Targeted ablation mice led reduced lung eosinophilia after allergen challenge, with broadly reprogrammed immunoactivation...
Eosinophilic esophagitis (EE) is an emerging disease associated with both food and respiratory allergy characterized by extensive esophageal tissue remodeling abnormal gene expression, including increased IL-13. We investigated the ability of airway IL-13 to induce EE-like changes. Mice pulmonary (but not esophageal) overexpression evidenced accumulation developed prominent epithelial hyperplasia, angiogenesis, collagen deposition, circumference. induced notable changes in transcripts that...
Loss of esophageal SPINK7 unleashes protease activity, impairs barrier function, and induces proinflammatory responses.
Abstract Accumulating data have indicated a fundamental role of eosinophils in regulating adipose tissue homeostasis. Here, we performed whole-genome RNA sequencing the small intestinal tract, which suggested presence impaired lipid metabolism eosinophil-deficient ΔdblGATA mice. mice fed high-fat diet (HFD) showed reduced body fat mass, enlargement adipocytes, decreased expression adipogenic genes, and developed glucose intolerance. HFD induced accumulation perigonadal white tissue....
Recent data associate eosinophilic esophagitis (EoE) with IgG4 rather than IgE, but its significance and function have not been determined. Our aims were to measure esophageal levels determine functional correlations as assessed by histologic transcriptome analyses.This case-control study included pediatric subjects EoE (≥15 eosinophils/HPF) non-EoE controls. Protein lysates analyzed for IgA, IgM, IgG1-IgG4 using the Luminex 100 system; IgE was quantified ELISA. Esophageal biopsies scored...
CD22 is currently recognized as a B cell-specific Siglec and has been exploited therapeutically with humanized anti-CD22 mAb having used against cell leukemia. In this study, tissue-specific eosinophil mRNA microarray analysis identified that transcript levels of murine gastrointestinal (GI) eosinophils are 10-fold higher than those lung eosinophils. To confirm the data at protein level, we developed FACS-based protocol designed to phenotype live GI isolated from lamina propria. Indeed,...
Most in vivo studies of granulocytes draw conclusions about their trafficking based on examination steady-state tissue/blood levels, which result from a combination tissue homing, survival, and egress, rather than direct cellular trafficking. Herein, we developed unique cell transfer system involving the adoptive genetically labeled, bone-marrow–derived granulocyte population (eosinophils) into an elicited inflammatory site, allergic lung. A dual polychromatic FACS-based biomarker-labeling...
MiR-21 is one of the most up-regulated miRNAs in multiple allergic diseases associated with eosinophilia and has been shown to positively correlate eosinophil levels. Herein, we show that miR-21 during IL-5-driven differentiation from progenitor cells vitro. Targeted ablation leads reduced cell growth. Furthermore, miR-21(-/-) have increased apoptosis as indicated by levels annexin V positivity compared miR-21(+/+) cells. Indeed, mice blood vivo colony forming unit capacity bone marrow....
The prevalence of allergic diseases is on the rise, yet environmental factors that contribute to this increase are still being elucidated. Laundry detergent (LD) contains cytotoxic ingredients including microbial enzymes continuously comes into contact with skin starting in infancy. An impaired barrier has been suggested as a route sensitization. We hypothesized exposure LD damages resulting systemic sensitization allergens enter through barrier. Mouse samples exposed vitro proteases or...
Eosinophilia and its cellular activation are hallmark features of asthma, as well other allergic/Th2 disorders, yet there few, if any, reliable surface markers eosinophil activation. We have used a FACS-based genome-wide screening system to identify transcriptional alterations in murine lung eosinophils recruited activated by pulmonary allergen exposure. Using relatively stringent screen with false-positive correction, we identified 82 candidate genes that could serve and/or pathogenic...
ABSTRACT In Trypanosoma brucei , two classes of transcripts are produced from distinct mitochondrial genome components. Guide RNAs (gRNAs) usually minicircle encoded and exist as primary transcripts, while the maxicircle-encoded rRNAs mRNAs processed a polycistronic precursor. The genes for gRNAs gMURF2-II gCYb(560) each have uncommon kinetoplast DNA (kDNA) locations that not typically associated with transcription initiation events. We demonstrate conserved maxicircle gRNA has an unusual...
Abstract Eosinophilic esophagitis (EoE) is a Th2 cytokine–associated disease characterized by eosinophil infiltration, epithelial cell hyperplasia, and tissue remodeling. Recent studies highlighted major contribution for IL-13 in EoE pathogenesis. Paired Ig-like receptor B surface immune-inhibitory that expressed eosinophils postulated to regulate development migration. We report Pirb upregulated the esophagus after inducible overexpression of (CC10-Il13Tg mice) overexpressed esophageal...