A5004085829- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Nicotinic Acetylcholine Receptors Study
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging and Analysis
- Shoulder Injury and Treatment
- Bone fractures and treatments
- Cell Adhesion Molecules Research
- Hip disorders and treatments
- Retinal Development and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Toxoplasma gondii Research Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Clinical practice guidelines implementation
- Pharmacological Effects and Assays
- Heart Rate Variability and Autonomic Control
- Orthopaedic implants and arthroplasty
- Bone health and treatments
- Cardiovascular and exercise physiology
- Medical Imaging Techniques and Applications
- Pharmacological Receptor Mechanisms and Effects
- Pulmonary Hypertension Research and Treatments
University of Michigan
2019-2025
Orthopaedic Research Laboratories
2021-2023
Michigan Medicine
2022
Hospital for Special Surgery
2020
Synovium is acutely affected following joint trauma and contributes to post-traumatic osteoarthritis (PTOA) progression. Little known about discrete cell types molecular mechanisms in PTOA synovium. We aimed describe synovial populations their dynamics PTOA, with a focus on fibroblasts. also sought define of Wnt/β-catenin signalling, given its emerging importance arthritis.We subjected mice non-invasive anterior cruciate ligament rupture as model human injury. performed single-cell...
Knee joints are densely innervated by nociceptors. In human knees and rodent models, sprouting of nociceptors has been reported in late-stage osteoarthritis (OA). Here, we sought to describe progressive nociceptor remodeling early OA, using four distinct experimental mouse models.
The intrinsic ability of injured neurons to degenerate and regenerate their axons facilitates nervous system repair, however this is not engaged in all injury locations. Here we investigate the regulation a conserved axonal response pathway with respect location damage branched motoneuron Drosophila larvae. dileucine zipper kinase DLK, (also known as MAP3K12 mammals Wallenda (Wnd) ), key regulator diverse responses injury. In three different populations motoneurons, observed same striking...
CD13, an ectoenzyme on myeloid and stromal cells, also circulates as a shed, soluble protein (sCD13) with powerful chemoattractant, angiogenic, arthritogenic properties, which require engagement of G protein-coupled receptor (GPCR). Here we identify the GPCR that mediates sCD13 actions bradykinin B1 (B1R). Immunofluorescence immunoblotting verified high expression B1R in rheumatoid arthritis (RA) synovial tissue fibroblast-like synoviocytes (FLSs), demonstrated binding to B1R. Chemotaxis,...
Abstract Assess acute alterations in bone turnover, microstructure, and histomorphometry following noninvasive anterior cruciate ligament rupture (ACLR). Twelve female Lewis rats were randomized to receive ACLR or Sham loading ( n = 6/group). In vivo μCT was performed at 3, 7, 10, 14 days postinjury quantify compartment‐dependent subchondral (SCB) epiphyseal trabecular remodeling. Near‐infrared (NIR) molecular imaging used measure anabolism (800 CW BoneTag) catabolism (Cat K 680 FAST)....
ABSTRACT Objective Knee joints are densely innervated by nociceptors. Sprouting of nociceptors has been reported in late-stage osteoarthritis (OA), both human knees and rodent models. Here, we sought to describe progressive nociceptor remodeling four mouse models knee OA, capturing early disease. Methods Sham surgery, destabilization the medial meniscus (DMM), partial meniscectomy (PMX), or non-invasive anterior cruciate ligament rupture (ACLR) was performed right 10-12-week old male C57BL/6...
ABSTRACT Objectives Synovium is acutely affected following joint trauma and contributes to post-traumatic osteoarthritis (PTOA) progression. Little known about discrete cell types molecular mechanisms in PTOA synovium. We aimed describe synovial populations their dynamics PTOA, with a focus on fibroblasts. also sought define of Wnt/β-catenin signaling, given its emerging importance arthritis. Methods subjected mice non-invasive anterior cruciate ligament rupture as model human injury....
ABSTRACT Objective To elucidate sex differences in synovitis, mechanical sensitization, structural damage, bone remodeling, and the synovial transcriptome anterior cruciate ligament rupture (ACLR) mouse model of post-traumatic osteoarthritis (PTOA). Methods Male female 12-week-old C57Bl/6 mice were randomized to noninvasive ACLR or sham (n=9/sex/group/timepoint). Knee hyperalgesia, allodynia, intra-articular MMP activity (via intravital imaging) measured longitudinally. Trabecular...
Abstract The intrinsic ability of injured neurons to degenerate and regenerate their axons facilitates nervous system repair, however this is not engaged in all injury locations. Here we investigate the regulation a conserved axonal response pathway with respect location damage branched motoneuron Drosophila larvae. dileucine zipper kinase DLK, (also known as MAP3K12 mammals Wallenda (Wnd) ), key regulator diverse responses injury. In three different populations motoneurons, observed same...
The intrinsic ability of injured neurons to degenerate and regenerate their axons facilitates nervous system repair, however this is not engaged in all injury locations. Here we investigate the regulation a conserved axonal response pathway with respect location damage branched motoneuron Drosophila larvae. dileucine zipper kinase DLK, (also known as MAP3K12 mammals Wallenda (Wnd) ), key regulator diverse responses injury. In three different populations motoneurons, observed same striking...
The intrinsic ability of injured neurons to degenerate and regenerate their axons facilitates nervous system repair, however this is not engaged in all injury locations. Here we investigate the regulation a conserved axonal response pathway with respect location damage branched motoneuron Drosophila larvae. dileucine zipper kinase DLK, (also known as MAP3K12 mammals Wallenda (Wnd) ), key regulator diverse responses injury. In three different populations motoneurons, observed same striking...
ABSTRACT Objective Assess acute alterations in bone turnover, microstructure, and histomorphometry following noninvasive anterior cruciate ligament rupture (ACLR). Methods Twelve female Lewis rats were randomized to receive ACLR or Sham loading (n=6/group). I n vivo μCT was performed at 3, 7, 10, 14 days post-injury quantify compartment-dependent subchondral (SCB) epiphyseal trabecular remodeling. Near-infrared (NIR) molecular imaging used measure anabolism (800 CW BoneTag) catabolism (Cat K...
Background: Blood flow restriction therapy (BFRT) has been increasingly applied to improve athletic performance and injury recovery. Validation of BFRT lagged behind commercialization, currently the mechanism by which this acts is unknown. one type ischemic therapy, involves exercising with blood restriction. Repetitive muscle (RRMBF) another type, does not include exercise. Hypothesis/Purpose: The purpose was develop a rat model characterize changes contractility, evaluate local systemic...
Micro-computed tomography (µCT) and contrast-enhanced (CE)-µCT are powerful, widely-used modalities for 3D assessment of bone articular cartilage (AC) tissues, respectively.• Volume-of-interest (VOI) selection these tissues on µCT images generally requires manual contouring, which is time consuming leads to inter-and intraobserver variability.• Few automated segmentation techniques have been described due the complex shape AC underlying bone.• Atlas-based an effective technique simultaneous...
Objectives: Patients with Femoroacetabular impingement (FAI) have been shown to elevated circulating biomarkers of inflammation and cartilage catabolism, but little is known about whether corrective surgical intervention improves objective measures hip strength inflammation. This study measured several variables including pain, function, quality life, symptoms, strength, inflammatory in a cohort subjects undergoing FAI surgery postoperative (postop) rehabilitation. We hypothesized that...