A5069027062- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Spaceflight effects on biology
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Invertebrate Immune Response Mechanisms
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Micro and Nano Robotics
- Advanced Fluorescence Microscopy Techniques
- Plant Molecular Biology Research
- Muscle Physiology and Disorders
- Microfluidic and Bio-sensing Technologies
- Collagen: Extraction and Characterization
- Genetic Neurodegenerative Diseases
- Advanced Electron Microscopy Techniques and Applications
- Morphological variations and asymmetry
- Ophthalmology and Eye Disorders
- RNA Research and Splicing
- Mathematical Biology Tumor Growth
- Plant Reproductive Biology
- Glycogen Storage Diseases and Myoclonus
The University of Tokyo
2021-2025
Universal University
2023
Kyoto University
2012-2022
Japan Science and Technology Agency
2015
Material Sciences (United States)
2015
RIKEN Center for Brain Science
2007-2013
RIKEN
2008-2010
Nippon Soken (Japan)
2009
Graduate School USA
2003
Baruch S. Blumberg Institute
2003
Understanding the mechanisms regulating development requires a quantitative characterization of cell divisions, rearrangements, size and shape changes, apoptoses. We developed multiscale formalism that relates characterizations each process to tissue growth morphogenesis. Having validated on computer simulations, we quantified separately all morphogenetic events in Drosophila dorsal thorax wing pupal epithelia obtain comprehensive statistical maps linking scale dynamics. While globally...
Many epithelial tissues pack cells into a honeycomb pattern to support their structural and functional integrity. Developmental changes in cell packing geometry have been shown be regulated by both mechanical biochemical interactions between cells; however, it is largely unknown how molecular cellular dynamics tissue mechanics are orchestrated realize the correct robust development of hexagonal packing. Here, combining genetic perturbations along with live imaging Bayesian force inference,...
Little has been understood about the underlying mechanisms that generate morphological diversity of dendritic trees. Dendritic arborization neurons in Drosophila provide an excellent model system to tackle this question, and they are classified into classes I-IV order increasing arbor complexity. Here we have developed transgenic green fluorescent protein markers for class I or IV cells, which allowed time-lapse recordings birth embryo, its maturation processes larva, lesion-induced...
Abstract Background For the establishment of functional neural circuits that support a wide range animal behaviors, initial formed in early development have to be reorganized. One way achieve this is local remodeling circuitry hardwiring. To genetically investigate underlying mechanisms remodeling, one model system employs major group Drosophila multidendritic sensory neurons - dendritic arborization (da) which exhibit dramatic pruning and subsequent growth during metamorphosis. The 15 da...
Tissue remodeling involves collective cell movement, and proliferation apoptosis are observed in both development disease. Apoptosis considered to be closely correlated, but little is known about their coordinated regulation physiological tissue vivo. The replacement of larval abdominal epidermis with adult epithelium Drosophila pupae a simple model remodeling. During this process, epidermal cells (LECs) undergo replaced by histoblasts, which precursor cells. By analyzing caspase activation...
Dendritic tree morphology is a hallmark of cellular diversity in the nervous system, and Drosophila dendritic arborization (da) neurons provide an excellent model system to study its molecular basis. The da are classified into four classes I–IV order increasing branching complexity. A transcriptional regulator early B‐cell factor (EBF)/olfactory 1 (Olf‐1) family, Knot (Kn)/Collier (Col) expressed selectively class IV neurons, which generate most expansive complicated trees classes. Loss kn...
A continuum model of epithelial tissue mechanics was formulated using cellular-level mechanical ingredients and cell morphogenetic processes, including cellular shape changes rearrangements. This can include finite deformation, incorporates stress deformation tensors, which be compared with experimental data. Using this model, we elucidated dynamical behavior underlying passive relaxation, active contraction-elongation, shear flow. study provides an integrated scheme for the understanding...
Tissue growth and deformation result from the combined effects of various cellular events, including cell shape change, rearrangement, division, death. Resolving integrating these events is essential for understanding coordination tissue-scale by individual behaviors that are critical morphogenesis, wound healing, other collective phenomena. For epithelial tissues composed tightly connected cells, texture tensor method provides a unified framework quantifying tissue strains tracking cells in...
Abstract In order to understand how tissue mechanics shapes animal body, it is critical clarify cells respond and resist stress when undergoing morphogenetic processes, such as cell rearrangement. Here, we address the question in Drosophila wing epithelium, where anisotropic tension orients rearrangements. We found that localizes actin interacting protein 1 (AIP1), a cofactor of cofilin, on remodeling junction via cooperative binding cofilin F-actin. AIP1 promote turnover locally regulate...
Neurons develop distinctive dendritic morphologies to receive and process information. Previous experiments showed that competitive dendro-dendritic interactions play critical roles in shaping dendrites of the space-filling type, which uniformly cover their receptive field. We incorporated this finding constructing a new mathematical model, reaction dynamics two chemicals (activator suppressor) are coupled neuronal dendrite growth. Our numerical analysis determined conditions for branching...
We found an F-actin–binding small molecule serving as a fluorescent probe and scaffold for functional probes.
Measuring mechanical parameters in tissues, such as the elastic modulus of cell-cell junctions, is essential to decipher control morphogenesis. However, their vivo measurement technically challenging. Here, we formulated an image-based statistical approach estimate epithelial cells. Candidate models are constructed based on force-cell shape correlations obtained from image data. Substitution model functions into force-balance equations at cell vertex leads equation with respect model, by...
Abstract Tissues consist of cells with different molecular and/or mechanical properties. Measuring the forces and stresses in mixed-cell populations is essential for understanding mechanisms by which tissue development, homeostasis, disease emerge from cooperation distinct cell types. However, many previous studies have primarily focused their measurements on dissociated or aggregates a single-cell type, leaving mechanics largely unexplored. In present study, we aimed to elucidate influence...
Organs develop distinctive morphologies to fulfill their unique functions. We used Drosophila embryonic gonads as a model study how two different cell lineages, primordial germ cells (PGCs) and somatic gonadal precursors (SGPs), combine form one organ. developed membrane GFP marker image SGP behaviors live. These studies show that combination of shape changes inward movement anterior posterior SGPs leads the compaction spherical gonad. This process is disrupted in mutants actin regulator,...
<h2>Summary</h2> In vertebrates, newly emerging transformed cells are often apically extruded from epithelial layers through cell competition with surrounding normal cells. However, the underlying molecular mechanism remains elusive. Here, using phospho-SILAC screening, we show that phosphorylation of AHNAK2 is elevated in neighboring RasV12 soon after induction expression, which mediated by calcium-dependent protein kinase C. addition, transient upsurges intracellular calcium, call calcium...