A5100387564- Respiratory viral infections research
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- Viral Infections and Vectors
- Tracheal and airway disorders
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- interferon and immune responses
- Hepatitis C virus research
- HIV, Drug Use, Sexual Risk
- Congenital Diaphragmatic Hernia Studies
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Cancer Research and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Parvovirus B19 Infection Studies
- Pneumonia and Respiratory Infections
Shanghai University of Traditional Chinese Medicine
2022-2024
Shuguang Hospital
2024
Shenzhen Municipal People's Government
2022
AstraZeneca (United States)
2019-2020
AstraZeneca (Brazil)
2020
Shenzhen Center for Disease Control and Prevention
2017-2018
Guangzhou Center for Disease Control and Prevention
2014-2016
Serimmune (United States)
2016
University of Hong Kong
2014
Centers for Disease Control and Prevention
2014
Abstract Despite prolonged surveillance and interventions, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) influenza viruses continue to pose a global health burden. Thus, we developed chimpanzee adenovirus-based combination vaccine, AdC68-HATRBD, with dual specificity against SARS-CoV-2 virus. When used as standalone intranasal immunization AdC68-HATRBD induced comprehensive potent immune responses consisting of immunoglobin (Ig) G, mucosal IgA, neutralizing antibodies,...
Respiratory syncytial virus (RSV) is a significant cause of lower respiratory tract infection in infants and elderly. To understand the evolution neutralizing epitopes on RSV glycoprotein (G) fusion (F) proteins, we conducted multi-year surveillance program (OUTSMART-RSV) US. Analysis 1,146 samples from 2015-2017 revealed slight shift prevalence A (58.7%) to B (53.7%) between two seasons. was more prevalent elderly (52.9% 73.4%). Approximately 1% contained both viruses. All isolates were ON1...
The southeastern region of Yunnan province is a key site for drug trafficking and HIV-1 infection spread from the west Laos to China. To investigate prevalence hepatitis C virus (HCV) coinfection among injection users (IDUs) in Yunnan, three cohorts 285 addicts, including 242 IDUs 43 oral users, living cities Gejiu Kaiyuan county Yanshan were studied. HCV infections detected by enzyme-linked immunosorbent assay and/or polymerase chain reaction. Data on age, sex, risk behavior, use history,...
Purpose A virologic surveillance program for A(H7N9) virus was conducted from April 15, 2013 to February 14, 2014 in Guangzhou, aiming clarify the geographical distribution of viruses among live poultry markets (LPMs) and farms Guangzhou. Virological serological surveys workers were also evaluate risk poultry-to-human transmission virus. Methods 36 retail LPMs, 6 wholesale LPMs 8 involved our program. About 20 environmental samples obtained each site at every sampling time. Different...
Targeted surveillance for influenza A(H7N9) identified 24 cases of infection with this virus in Guangzhou, China, during April 1, 2013-March 7, 2014. The spectrum illness ranged from severe pneumonia to asymptomatic infection. Epidemiologic findings 2 family clusters highlight the importance rigorous close contact monitoring.
Abstract Background RSV is a leading cause of lower respiratory tract infection in infants. Monitoring glycoprotein sequences critical for understanding epidemiology and viral antigenicity the effort to develop anti‐RSV prophylactics therapeutics. Objectives The objective characterize circulating strains collected from infants South Africa during 2015‐2017. Methods A subset 150 RSV‐positive samples obtained HIV‐unexposed HIV‐exposed‐uninfected 2015 2017, were selected high‐throughput...
With the goal of developing a virus-like particle-based vaccine based on dense bodies (DB) produced by human cytomegalovirus (HCMV) infections, we evaluated scalable culture, isolation, and inactivation methods applied technically advanced assays to determine relative purity, composition, immunogenicity DB particles. Our results increase our understanding benefits disadvantages recover immunogenic inactivate contaminating viral indicate that (i) HCMV strain Towne replicates in MRC-5...
Cornus officinalis total glycosides (COTG) derived from the traditional Chinese medicine officinalis, is a natural immunosuppressant and has been extensively studied in immunomodulation immunosuppression. This study aimed to explore effects of COTG on granulomatous lobular mastitis (GLM) its associated mechanisms. Compared model group, effectively ameliorated histopathological damage breast tissue, reduced mammary gland suppuration, enhanced blood‐milk barrier. Additionally, treatment number...
Avian influenza A (H7N9) virus has caused several epidemics and infection in both human poultry. With mutation, the H7N9 gained its fifth endemic China. Early diagnosis is crucial for control of viral spread poultry prognosis infected patients. In this study, we developed evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay rapid detection hemagglutinin neuraminidase gene H7N9. Our H7-LFD-RPA N9-LFD-RPA were able to detect 32 fg nucleic acid which more...
COVID-19 has been a topic of interest since pandemic struck in 2019. The morbidity influenza tended to decrease due the measures prevent COVID-19. Indeed, seems be "ignored" this era However, not disappeared from scene. Presented here are two examples recent epidemics China and Australia. Possible interactions between discussed. Measures against may reduce contact with influenza, subsequently reducing adaptive immunity general population. Influenza might center stage right now, but...
No licensed vaccine is available for prevention of EBV-associated diseases, and robust, sensitive, high-throughput bioanalytical assays are needed to evaluate immunogenicity gp350 subunit-based candidate EBV vaccines. Here we have developed improved analytical tools such a vaccine’s pre-clinical clinical validation including gp350-specific antibody detection assay an EBV-GFP based neutralization measuring specific antibodies in human donors. The sensitivity our previously published...
Objective To analyze the substitutions at amino acid residues of neuraminidases (NAs) in influenza B virus strains isolated Jiangsu province from 2010 to 2012 and further understand genetic evolution NAs those strains. Methods Forty were screened out for this study. A two-step reverse transcription PCR (RT-PCR) was performed amply gene fragments encoding strains. The products purified then sequenced an ABI 3730XL Genetic Analyzer. evolutionary characteristics NA analyzed by using...
Objective Exploring the molecular characteristic of global and Shenzhen district H5N6 H7N9 influenza viruses HA untranslated regions(UTRs). Methods Mega7.0 DNAStar 7.1.0 were used to construct phylogenetic tree nucleotide analysis. Results From 2014 2015, 3 strains virus from compared with other H5NX viruses, homology HA-3’UTR was 77.4%-100%, which did not have obvious mutated sites. The H5N6-HA-5’UTR 91.7%-100%, sites 24 31 mutated. From 2013 2014, 11 H7NX H7N9-HA-5’UTR...
Objective To analyze the distribution and epidemiological features of avian influenza virus in external environments Shenzhen during 2015-2019. Methods The environment samples collected 2015-2019 were used to detect nucleic acids by real-time fluorescent PCR. The H5, H7 H9 subtypes analyzed for positive samples. A database was constructed data statistical methods. Results For 4 674 from December, 2015 May, 2019, 2 243 FluA with a rate 48.0%. For subtypes, numbers 673, 283 799,...
Abstract Background SARS-CoV-2 could infect people at all ages, and the viral shedding immunological features of children COVID-19 patients were analyzed. Methods Epidemiological information clinical data collected from 35 patients. Viral RNAs in respiratory fecal samples detected. Plasma 11 measured for 48 cytokines. Results 40% (14/35) showed asymptomatic infections, while pneumonia shown by CT scan occurred most cases (32/35, 91.43%). Elevated LDH, AST, CRP, neutropenia, leukopenia,...
This study aimed to explore the protective effect of Reduning Injection(RDN) on mice infected by influenza virus A/PR/8(PR8) and its immune regulatory roles during viral infection. In in vivo experiments, female C57 BL/6 were randomly divided into phosphate buffered saline(PBS) group, PR8-infected oseltamivir treatment group(OSV) RDN group. After 2 h PR8 infection, group gavaged with 30 mg·kg~(-1), those injected intraperitoneally 1.5 mL·kg~(-1)once per day for seven consecutive days. The...