A5101836650- Virus-based gene therapy research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- Cancer Research and Treatments
- Vector-Borne Animal Diseases
- Congenital Diaphragmatic Hernia Studies
- Pneumonia and Respiratory Infections
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Tracheal and airway disorders
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Cleft Lip and Palate Research
AstraZeneca (United States)
2018-2021
NGM Biopharmaceuticals (United States)
2020
AstraZeneca (United Kingdom)
2020
Serimmune (United States)
2012-2019
Boramae Medical Center
2016
Seoul National University
2016
PredictImmune
2015
Fudan University
2010
State Key Laboratory of Genetic Engineering
2010
Harbin Veterinary Research Institute
2003
A live attenuated influenza A/Vietnam/1203/2004 (H5N1) vaccine virus (VN04 ca) has receptor binding specificity to alpha2,3-linked sialosides (alpha2,3SAL), and a single dose induces minimal serum antibody response in mice ferrets. In contrast, A/Hong Kong/213/2003 (HK03 binds both alpha2,6SAL alpha2,3SAL generates stronger animals. Among the 9 amino acids that differed between two H5 HA1 proteins, several HK03-specific residues enabled VN04 ca bind receptors, but only removal of 158N...
<h3>Rationale</h3> Interleukin (IL)-33, a new member of the IL-1 family, is constitutively expressed in epithelial tissues and lymphoid organs plays an important role pathogenesis allergic disease. However, IL-33 chronic rhinosinusitis with nasal polyps (CRSwNP) remains unclear. <h3>Objective</h3> To investigate pathophysiology CRSwNP. <h3>Methods</h3> We investigated expression its cellular origins (NPs) human subjects by immunohistochemistry (IHC), quantitative reverse transcription PCR...
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection among infants and young children, resulting in annual epidemics worldwide. INFORM-RSV a multiyear clinical study designed to describe global molecular epidemiology RSV children under 5 years age by monitoring temporal geographical evolution current circulating strains, F protein antigenic sites, their relationships with features disease. During pilot season (2017–2018), 410 G-F gene sequences were...
The humoral and cellular immune responses elicited by the trivalent live attenuated influenza vaccine (LAIV) inactivated (TIV) were evaluated in ferret model, using newly developed immunological reagents assays. In contrast to TIV, which only induced primed animals, LAIV strong virus-specific serum antibody T-cell both naive influenza-seropositive animals. offered significant protection against a heterologous H1N1 virus challenge infection upper respiratory tract. Influenza immunoglobulin A...
Respiratory syncytial virus (RSV) is a significant cause of lower respiratory tract infection in infants and elderly. To understand the evolution neutralizing epitopes on RSV glycoprotein (G) fusion (F) proteins, we conducted multi-year surveillance program (OUTSMART-RSV) US. Analysis 1,146 samples from 2015-2017 revealed slight shift prevalence A (58.7%) to B (53.7%) between two seasons. was more prevalent elderly (52.9% 73.4%). Approximately 1% contained both viruses. All isolates were ON1...
Abstract Background Chronic obstructive pulmonary disease (COPD) is a heterogeneous characterized by frequent exacerbation phenotypes independent of stage. Increasing evidence shows that the microbiota plays role in progression and severity, but long-term international multicenter assessment variations viral bacterial communities as drivers exacerbations are lacking. Methods Two-hundred severe COPD patients from Europe North America were followed longitudinally for 3 years. We performed...
ABSTRACT Clinical development of a mesogenic strain Newcastle disease virus (NDV) as an oncolytic agent for cancer therapy has been hampered by its select status due to pathogenicity in avian species. Using reverse genetics, we have generated lead candidate NDV based on the NDV-73T that is no longer classified clinical development. This recombinant modification at fusion protein (F) cleavage site reduce efficiency F and insertion 198-nucleotide sequence into HN-L intergenic region, resulting...
Oncolytic virus (OV) therapy is an emerging approach with the potential to redefine treatment options across a range of cancer indications and in patients who remain resistant existing standards care, including immuno-oncology (IO) drugs. MEDI5395, recombinant Newcastle disease (NDV), engineered express granulocyte-macrophage colony-stimulating factor (GM-CSF), exhibits potent oncolytic activity. It was hypothesized that activation immune cells by coupled its activity, would enhance priming...
Background Toll-like receptor (TLR)4 agonists are known potent immunostimulatory compounds. These compounds can be formulated as part of novel adjuvants to enhance vaccine medicated immune responses. However, the contribution formulation innate in vivo activity TLR4 agonist is not well understood. Methodology and Principal Findings We evaluated synthetic Glucopyranosyl Lipid A (GLA) for its effects on molecular cellular responses murine model. Microarray techniques were used compare GLA an...
Priming immunization plays a key role in protecting individuals or populations to influenza viruses that are novel humans. To identify the most promising vaccine priming strategy, we have evaluated different prime-boost regimens using inactivated, DNA and live attenuated vaccines ferrets. Live A/Vietnam/1203/2004 (H5N1) candidate (LAIV, VN04 ca) primed ferrets efficiently while inactivated H5N1 could not prime immune response seronegative unless an adjuvant was used. However, H5 HA alone as...
Since it is difficult to predict which influenza virus subtype will cause an pandemic, important prepare vaccines against different subtypes and evaluate the safety immunogenicity of candidate in preclinical clinical studies prior a pandemic. In addition infecting humans, H3 viruses commonly infect pigs, horses, avian species. We selected 11 swine, equine, evaluated their kinetics replication ability induce broadly cross-reactive antibody response mice ferrets. The swine equine replicated...
Newcastle disease virus (NDV) is an oncolytic being developed for the treatment of cancer. Following infection a human ovarian cancer cell line (OVCAR3) with recombinant low-pathogenic NDV, persistent was established in subset tumor cells. Persistently infected (PI) cells exhibited resistance to superinfection NDV and antiviral state, as demonstrated by upregulation interferon interferon-induced genes such myxoma gene 1 (Mx1) retinoic acid-inducing gene-I (RIG-I). Viruses released from PI...
Abstract Background Respiratory syncytial virus (RSV) is a global cause of severe respiratory morbidity and mortality in infants. While preventive therapeutic interventions are being developed, including antivirals, vaccines monoclonal antibodies, little known about the molecular epidemiology RSV. INFORM prospective, multicenter, clinical study performed by ReSViNET to investigate worldwide diversity RSV isolates collected from children less than 5 years age. Methods The 17 countries...
Background Respiratory syncytial virus (RSV) is an established cause of serious lower respiratory disease in infants, elderly and high-risk populations. The OUTSMART surveillance program aims to characterize patient populations currently circulating RSV strains, monitor temporal geographic evolution F G proteins the U.S. Methods 2016–17 study collected RSV-positive samples from 25 RSVAlert® laboratories 4 regions Puerto Rico during November 2016 through March 2017. Frequencies A B subtypes...
Abstract Background RSV is a leading cause of lower respiratory tract infection in infants. Monitoring glycoprotein sequences critical for understanding epidemiology and viral antigenicity the effort to develop anti‐RSV prophylactics therapeutics. Objectives The objective characterize circulating strains collected from infants South Africa during 2015‐2017. Methods A subset 150 RSV‐positive samples obtained HIV‐unexposed HIV‐exposed‐uninfected 2015 2017, were selected high‐throughput...
Live attenuated influenza vaccine (LAIV) candidates of the H7 subtype, A/Netherlands/219/03 (H7N7, NL03 ca) and A/chicken/British Columbia/CN-6/2004 (H7N3, BC04 ca), were evaluated for their receptor binding specificity immunogenicity in ferrets. The ca virus exhibited α2,3-SA α2,6-SA dual preference while preferentially bound to α2,3-SA. Substitution Q226 G228 (Q-G) by L226 S228 (L-S) residues HA improved ca. viruses with L-S retained attenuation phenotype. replicated more efficiently than...
H2 influenza viruses have not circulated in humans since 1968, and therefore a significant portion of the population would be susceptible to infection should reemerge. continue circulate avian reservoirs worldwide, these are potential source from which could emerge. Three reassortant cold-adapted (ca) pandemic vaccine candidates with hemagglutinin (HA) neuraminidase (NA) genes derived wild-type A/Japan/305/1957 (H2N2) (Jap/57), A/mallard/6750/1978 (mal/78), or A/swine/MO/4296424/2006 (H2N3)...
Live attenuated influenza H5N1 vaccines have been produced and evaluated in mice ferrets that were never exposed to A virus infection (Suguitan et al., Plos Medicine, e360:1541, 2006). However, the preexisting heterosubtypic immunity on live vaccine induced immune response has not evaluated.Primary recall B cell responses viruses examined using a sensitive antigen-specific ELISpot assay investigate effect of development H5N1-specific ferrets. A/Hong Kong/213/03 A/Vietnam/1203/04 measurable...
We have developed an oncolytic Newcastle disease virus (NDV) that has potent in vitro and vivo anti-tumor activities attenuated pathogenicity chickens. In this ex study using the same recombinant NDV backbone with GFP transgene (NDV-GFP, designated as rNDV), we found rNDV induces maturation of monocyte-derived immature dendritic cells (iDCs) by both direct indirect mechanisms, which promote development antigen-specific T cell responses. Addition directly to iDCs culture induced DC...
Newcastle disease virus (NDV) is being developed as an oncolytic for virotherapy. In this study we analysed the regulation of complement-mediated inactivation a recombinant NDV in different host cells. grown human cells was less sensitive to than embryonated chicken eggs. Additionally, produced from HeLa-S3 more resistant complement 293F cells, which correlated with higher expression and incorporation regulatory proteins (CD46, CD55 CD59) into virions Further analysis NDVs individually...
A recombinant Newcastle Disease Virus (NDV), encoding either a human (NDVhuGM-CSF, MEDI5395) or murine (NDVmuGM-CSF) GM-CSF transgene, combined broad oncolytic activity with the ability to significantly modulate genes related immune functionality in tumor cells. Replication lines was diminished relative Nonetheless, intratumoral injection of NDVmuGM-CSF conferred antitumor effects three syngeneic models vivo; efficacy further augmented by concomitant treatment anti-PD-1/PD-L1 T-cell...