Copper chelator cuprizone (CPZ) is neurotoxicant, which selectively disrupts oligodendroglial respiratory chain, leading to oxidative stress and subsequent apoptosis. Demyelination is, however, followed by spontaneous remyelination owing the activation of intrinsic CNS repair mechanisms. To explore participation metallothioneins (MTs) in these processes, this study we analyzed expression profiles MT-I/II their receptor megalin (low-density lipoprotein related protein-2) brain mice subjected...

Highlights•OPN and MT-I+II are potent modulators of inflammatory regenerative processes in diseases the nervous system.•During first attack EAE transcription expression both proteins arose spinal cord brain.•In inflamed generated OPN fragments low molecular weight.•Multiple αvβ3+ neurons co-express contain granular signals secretory vesicles Golgy.•OPN/MT interactions may contribute to tissue remodeling during an autoimmune attack.AbstractOsteopontin (OPN), extracellular matrix (ECM)...

The genotoxic effects of occupational exposure to ionizing and non-ionizing radiation were investigated in 25 physicians nurses working hospitals 20 individuals at radio-relay stations. Examination was conducted by chromosome aberration analysis peripheral blood lymphocytes. data showed that total number aberrations people exposed radio-frequency (4.08 +/- 0.37 4.35 0.5 on 200 scored metaphases, respectively) almost equally higher than those non-irradiated subjects. increase proportion the...

To elucidate the role of metallothioneins (MTs) in pathomechanisms autoimmune CNS disorders we estimated expression MTs I+II and tissue concentrations Zn2+ Cu2+ brain, spinal cord (SC) liver during periods attacks remissions chronic relapsing experimental encephalomyelitis (CR-EAE). Disease was induced genetically susceptible Dark Agouti (DA) rats by subcutaneous injection bovine brain homogenate CFA. Control were treated with CFA or saline solution. The data, obtained clinical assessment,...

Metallothioneins (MTs) are small, cysteine-rich proteins which have been implicated in various forms of stress providing cytoprotective action against oxidative injury, DNA damage and apoptosis. Owing to their high affinity for physiological metals, such as zinc copper MTs also critical components regulatory involved cell growth multiplication, well the maintenance immune homeostasis. To elucidate role pathomechanisms autoimmune CNS disorders we estimated expression MT I+II content free Zn...

Background : Cell‐wall components of Gram‐positive and Gram‐negative bacteria induce the production cytokines in human peripheral blood mononuclear cells. These are main mediators local or systemic inflammatory reaction that can contribute to development innate immunity. Aims This study was performed analyze involvement CD14 molecule activation monocytes by peptidoglycan monomer (PGM) obtained biosynthesis from culture fluid penicillin‐treated Brevibacterium divaricatum NRLL-2311 . Methods...

Tissue disintegration after injury leads, in the endoplasmic reticulum (ER), to activation of adaptive pathways known as ER stress response. It is directed correction unfolded proteins and proteasome-dependent ER-associated degradation misfolded proteins, but induces also a rapid natural immunity, since resident heat shock protein-gp96 acts not only molecular chaperone, strong adjuvant, able cross-present antigenic peptides onto MHC class I or II pathways. Analyzing its potential role...

Inflammatory demyelinating diseases such as multiple sclerosis and experimental autoimmune encephalomyelitis (EAE) are often followed by cognitive deficits associated with the neuronal injury, synaptic loss altered neurogenesis within hippocampus. Changes depend on genetic epigenetic factors that ensure cellular environmental homeostasis regulate interactions of immunocompetent, glial neural cells. Owing to high impact stress proteins these processes, in this study we compared protein...

Abstract Metallothioneins (MTs) are phylogenetically old cysteine-rich proteins, which implicated in a variety of physiological and pathological processes. Their growth-regulating, anti-apoptotic anti-inflammatory functions have been attributed not only to intracellular free radical scavenging zinc copper regulation but also the ability secreted MT bind on surface lipoprotein receptor-megalin/LRP2, enables endocytosis MT-I/II wide range other functionally distinct ligands. In present study,...

<b><i>Objectives:</i></b> Compared to the Dark Agouti (DA), Albino Oxford (AO) rat strain exhibits lower susceptibility induction of experimental autoimmune encephalomyelitis (EAE). Here, we investigated potential contribution heavy metal-binding proteins metallothioneins (MTs) I/II these effects. <b><i>Methods:</i></b> Rats were immunized with bovine brain homogenate emulsified in complete Freund's adjuvant or only adjuvant. The expression...

We examined the effect of dietary fats on kinetics liver regeneration after one-third partial hepatectomy (PH) in male Balb/c mice. Corn and olive supplemented diets were chosen as a model n-6 n-9 diets. Phospholipid fatty acids determined by gas chromatography (GC) previous fractionation solid-phase extraction using aminopropyl (NH2) column, data analysed nonparametric Kruskal-Wallis test. Diet enriched with corn oil did not affect balance lipid disorders during 1/3 PH. Desaturases activity...

In a search of peripheral factors that could be responsible for the discrepancy in susceptibility to EAE Albino Oxford (AO) and Dark Agouti (DA) rats, we estimated expression metallothioneins I/II (MT), heat shock protein-gp96, interleukin (IL)-6, transforming growth factor (TGF)- β livers these animals. Rats were immunized with bovine brain homogenate (BBH) emulsified complete Freund adjuvant (CFA) or only CFA. Western blot immunohistochemical analyses done on day 12 after immunization, as...

Environmental airborne pollution has been repeatedly shown to affect multiple aspects of brain and cardiopulmonary function, leading cognitive behavioral changes the pronounced inflammatory response in respiratory airways. Since cellular defense system important role might have stress proteins-metallothionein (MT)-I MT-II, which are involved sequestration dispersal metal ions, regulation biosynthesis activities zinc-dependent transcription factors, as well protection from reactive oxygen...