A5016989465- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Viral Infections and Vectors
- Neonatal and fetal brain pathology
- Cancer Research and Treatments
- Virus-based gene therapy research
- COVID-19 Impact on Reproduction
- Immunotherapy and Immune Responses
- Global Maternal and Child Health
- Veterinary Oncology Research
- Bacillus and Francisella bacterial research
- Cerebral Palsy and Movement Disorders
- Infant Development and Preterm Care
- Renin-Angiotensin System Studies
- Poxvirus research and outbreaks
- Invertebrate Immune Response Mechanisms
- Neuropeptides and Animal Physiology
- Axon Guidance and Neuronal Signaling
- Phagocytosis and Immune Regulation
- Extracellular vesicles in disease
- Heart Rate Variability and Autonomic Control
- Autism Spectrum Disorder Research
- vaccines and immunoinformatics approaches
- Veterinary Orthopedics and Neurology
- Hair Growth and Disorders
University of Wisconsin–Madison
2015-2025
Colorado State University
2005
ZIKV was first identified in 1947 Africa, but most of our knowledge is based on studies the distinct Asian genetic lineage, which caused outbreak Americas 2015 to 2016. In its recent update, WHO stated that improved understanding African-lineage pathogenesis during pregnancy must be a priority.
Infection with clade I Mpox virus (MPXV) results in adverse pregnancy outcomes, yet the potential for vertical transmission resulting fetal harm IIb MPXV, that is currently circulating Western Hemisphere, remains unknown. We sought to establish a pregnant rhesus macaque model of MPXV infection early gestation inoculation understand if and outcomes. Three macaques were inoculated intradermally 1.5 x 10 5 plaque forming units (PFU) near gestational day (GD) 30 animals monitored viremia...
Angiotensin II increases blood pressure and stimulates thirst sodium appetite in the brain. It also secretion of aldosterone from adrenal zona glomerulosa epinephrine medulla. The rat has 3 subtypes angiotensin receptors: AT1a, AT1b, AT2. mRNAs for all three occur To immunohistochemically differentiate these receptor subtypes, rabbits were immunized with C-terminal fragments to generate subtype-specific antibodies. Immunofluorescence revealed AT1a AT2 receptors AT1b immunofluorescence was...
Concerns have arisen that pre-existing immunity to dengue virus (DENV) could enhance Zika (ZIKV) disease, due the homology between ZIKV and DENV observation of antibody-dependent enhancement (ADE) among serotypes. To date, no study has examined impact on pathogenesis during pregnancy in a translational non-human primate model. Here we show macaques with prior DENV-2 exposure had higher burden vRNA maternal-fetal interface tissues as compared DENV-naive macaques. However, detectable...
Congenital Zika virus (ZIKV) infection can result in birth defects, including malformations the fetal brain and visual system. There are two distinct genetic lineages of ZIKV: African Asian. Asian-lineage ZIKVs have been associated with adverse pregnancy outcomes humans; however, recent evidence from experimental models suggests that African-lineage viruses also be vertically transmitted cause harm.
Abstract Infection with clade I Mpox virus (MPXV) results in adverse pregnancy outcomes, yet the potential for vertical transmission resulting fetal harm IIb MPXV, that is currently circulating Western Hemisphere, remains unknown. We established a rhesus macaque model of MPXV early gestation inoculation. Three pregnant macaques were inoculated intradermally 1.5 × 10^5 plaque forming units (PFU) near gestational day (GD) 30 and animals monitored viremia maternal well-being. Animals euthanized...
Countermeasures against Zika virus (ZIKV), including vaccines, are frequently tested in nonhuman primates (NHP). Macaque models important for understanding how ZIKV infections impact human pregnancy due to similarities placental development. The lack of consistent adverse outcomes ZIKV-affected pregnancies poses a challenge macaque studies where group sizes often small (4-8 animals). Studies animal suggest that African-lineage viruses can cause more frequent and severe fetal outcomes. No...
Zika virus (ZIKV) can be transmitted vertically from mother to fetus during pregnancy, resulting in a range of outcomes including severe birth defects and fetal/infant death. Potential pathways vertical transmission utero have been proposed but remain undefined. Identifying the timing routes ZIKV may help us identify when interventions would most effective. Furthermore, understanding what barriers overcomes effect improve models for evaluating infection by other pathogens pregnancy. To...
Abstract Lysozymes are enzymes characterized by their ability to break down bacterial cell walls. In insects certain lysozymes only found in the midgut, whereas others haemolymph and fat body after immune challenge. We identified two lysozyme‐encoding cDNAs from Aedes aegypti. Both deduced protein sequences basic nature, contain 148 amino acids including eight highly conserved cysteine residues, genomic a single intron. Transcriptional profiles indicated that predominant form is...
Flavin-containing monooxygenases (FMOs) play significant roles in the metabolism of drugs and endogenous or foreign compounds. In this study, regional distribution FMO isoforms 1, 3, 4 was investigated male Sprague-Dawley rat liver kidney using immunohistochemistry (IHC). Rabbit polyclonal antibodies to FMO1 FMO4, developed anti-peptide technology, commercial anti-human FMO3 antibody were used; specificities verified Western blotting, immunoprecipitation, IHC. liver, highest immunoreactivity...
Congenital Zika virus (ZIKV) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. ZIKV-related neuropathogenesis, predictors defects, and deficits are not well defined people. Here we assess the methodological statistical feasibility congenital ZIKV macaque model for identifying infant neurobehavior brain abnormalities that may underlie We inoculated five pregnant macaques with mock-inoculated one first trimester. Following...
Infants exposed to Zika virus (ZIKV) prenatally may develop birth defects, developmental deficits, or remain asymptomatic. It is unclear why some infants are more affected than others, although enhancement of maternal ZIKV infection via immunity an antigenically similar virus, dengue (DENV), play a role. We hypothesized that DENV worsen prenatal and deficits in offspring. utilized translational macaque model examine how influences ZIKV-exposed infant neurodevelopment the first month life....
This study describes the development of an human granulocyte–macrophage colony-stimulating factor DNA cationic-lipid complexed autologous tumour cell vaccine (hGM-CSF CLDC ATCV) and its implementation, following a chemotherapy treatment protocol, in randomized, placebo-controlled, double-blinded clinical trial pet dogs with naturally occurring lymphoma. We hypothesized that use this would result antitumour immune response leading to improved first remission duration overall survival B-cell...
Canine malignant melanoma (CMM) resembles human in terms of metastatic behavior, refractoriness to standard therapy, and tumor antigen expression but it is largely unknown how CMM with regard molecular pathogenesis cellular signaling. No attempt has been made systematically define the repertoire tyrosine kinases (TKs) expressed CMM. This study used a reverse transcription-PCR display technique evaluate multiple TKs 17CM98 cell line. RT-PCR was performed using degenerate primers coding for...
There are currently no approved drugs to treat Zika virus (ZIKV) infection during pregnancy. Hyperimmune globulin products such as VARIZIG and WinRho FDA-approved conditions pregnancy Varicella Zoster Rh-incompatibility. We administered ZIKV-specific human immune a treatment in pregnant rhesus macaques one day after subcutaneous ZIKV infection. All animals controlled viremia following the generated robust levels of anti-Zika antibodies their blood. No adverse fetal or infant outcomes were...
ABSTRACT Following the Zika virus (ZIKV) outbreak in Americas, ZIKV was causally associated with microcephaly and a range of neurological developmental symptoms, termed congenital syndrome (CZS). The isolates responsible for this belonged to Asian lineage ZIKV. However, in-vitro in-vivo studies assessing pathogenesis African-lineage demonstrated that often replicated high titer caused more severe pathology than Asian-lineage isolates. To date, translational model, particularly during...
Abstract Zika virus (ZIKV) can be transmitted vertically from mother to fetus during pregnancy, resulting in a range of outcomes, including severe birth defects and fetal/infant death. Potential pathways vertical transmission utero have been proposed but remain undefined. Identifying the timing routes ZIKV may help us identify when interventions would most effective. Furthermore, understanding what barriers overcomes effect improve models for evaluating infection by other pathogens...
Introduction Zika virus (ZIKV) infection during pregnancy results in a spectrum of birth defects and neurodevelopmental deficits prenatally exposed infants, with no clear understanding why some pregnancies are more severely affected. Differential control maternal ZIKV may explain the adverse outcomes. Methods Here, we investigated whether magnitude breadth ZIKV-specific antibody response is associated better virologic using rhesus macaque model prenatal infection. We inoculated 18 dams an...
Countermeasures against Zika virus (ZIKV), including vaccines, are frequently tested in nonhuman primates (NHP). Macaque models important for understanding how ZIKV infections impact human pregnancy due to similarities placental development. The lack of consistent adverse outcomes ZIKV-affected pregnancies poses a challenge macaque studies where group sizes often small (4-8 animals). Studies animal suggest that African-lineage viruses can cause more frequent and severe fetal outcomes. No...