A5068213193- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Advanced Biosensing Techniques and Applications
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Limits and Structures in Graph Theory
- Molecular spectroscopy and chirality
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Ethics and Social Impacts of AI
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- Biochemical and Structural Characterization
- Drilling and Well Engineering
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Advanced Graph Theory Research
- Mosquito-borne diseases and control
- Oil and Gas Production Techniques
- Virology and Viral Diseases
- Graph theory and applications
- Venomous Animal Envenomation and Studies
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Vector-borne infectious diseases
La Roche College
2015-2020
Roche (United States)
2019
Roche (Sweden)
2019
Jensen Hughes (United States)
2000-2007
Christ University
2000-2007
Tick-borne diseases are the most common vector-borne in United States, with serology being primary method of diagnosis. We developed first multiplex, array-based assay for serodiagnosis tick-borne called TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses 8 major pathogens present including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, miyamotoi, Ehrlichia chaffeensis, Rickettsia rickettsii, Heartland virus and Powassan virus. Each contains...
Zika virus (ZIKV) is implicated in fetal stillbirth, microcephaly, intracranial calcifications, and ocular anomalies following vertical transmission from infected mothers. In adults, infection may trigger autoimmune inflammatory polyneuropathy. Transmission most commonly follows the bite of Aedes mosquitoes but also occur through sexual intercourse or receipt blood products. Definitive diagnosis detection viral RNA possible serum plasma within 10 days disease onset, whole 3 weeks semen for...
Abstract Phage display is a prominent screening technique with multitude of applications including therapeutic antibody development and mapping antigen epitopes. In this study, phages were selected based on their interaction patient serum exhaustively characterised by high-throughput sequencing. A bioinformatics approach was developed in order to identify peptide motifs interest clustering contrasting control samples. Comparison samples confirmed major issue phage display, namely the...
Abstract T-cell bispecific antibodies (TCBs) crosslink tumor and T-cells to induce cell killing. While TCBs are very potent, on-target off-tumor toxicity remains a challenge when selecting targets. Here, we describe protease-activated anti-folate receptor 1 TCB (Prot-FOLR1-TCB) equipped with an anti-idiotypic anti-CD3 mask connected the Fab through protease-cleavable linker. The potency of this Prot- FOLR1-TCB is recovered following protease-cleavage linker releasing scFv. In vivo,...
Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs): SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report identification humoral immune responses to peptides that may enable discrimination between exposure other HCoVs. We used a high-density peptide microarray plasma samples collected at two time points from 50 subjects with confirmed qPCR, in 2004-2005 11 IgG antibodies seasonal (HCoV), 10 healthy...
Abstract Snakebite envenoming is a serious condition requiring medical attention and administration of antivenom. Current antivenoms are antibody preparations obtained from the plasma animals immunised with whole venom(s) contain antibodies against snake venom toxins, but also other antigens. In order to better understand molecular interactions between antivenom epitopes on high-throughput immuno-profiling study all manually curated toxins Dendroaspis species selected African Naja was...
Objective Autoantibodies against citrullinated proteins are found in 64–89% of rheumatoid arthritis ( RA ) patients, with 88–99% specificity. This study was undertaken to create an unbiased, comprehensive profile serum antibodies the human proteome, including citrullinome and homocitrullinome, using a high‐density peptide array. Methods Our array, consisting >4.6 million peptides, contained entire annotated proteome. The 20,246 were represented as overlapping 16‐mer peptides. In addition...
Considerable efforts have been made to develop technologies for selection of peptidic molecules that act as substrates or binders a protein interest. Here we demonstrate the combination rational peptide array library design, parallel screening and stepwise evolution, discover novel hotspots. These hotspots can be systematically evolved create high-affinity, high-specificity binding peptides target in reproducible digitally controlled process. The method applied synthesize both linear cyclic...
The majority of non-small-cell lung cancer (NSCLC) cases are diagnosed at advanced stages, primarily because earlier stages the disease either asymptomatic or may be attributed to other causes such as infection long-term effects from smoking. Therefore, early detection NSCLC would likely increase response and survival rates due timely intervention. Here, we utilize a novel approach based on whole genome-derived tiled peptide arrays identify epitopes associated with autoantibody reactivity in...
Coxiella burnetii is an important zoonotic bacterial pathogen of global importance, causing the disease Q fever in a wide range animal hosts. Ruminant livestock, particular sheep and goats, are considered main reservoir human infection. Vaccination key control measure, two commercial vaccines based on formalin-inactivated C. bacterins currently available for use livestock humans. However, their deployment limited due to significant reactogenicity individuals previously sensitized antigens....
Development and Utilization of a Bit Based Data Acquisition System in Hard Rock PDC Applications Paul E. Pastusek; Pastusek Hughes Christensen Co. Search for other works by this author on: This Site Google Scholar Eric Sullivan; Sullivan Thomas Mitchell Harris Paper presented at the SPE/IADC Drilling Conference, Amsterdam, The Netherlands, February 2007. Number: SPE-105017-MS https://doi.org/10.2118/105017-MS Published: 20 2007 Connected Content Related to: A Bit-Based Data-Acquisition...
Abstract Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity to the human coronaviruses (HCoV) SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report identification humoral immune responses other HCoV peptides that can be used detect asymptomatic, mild and, severe infections, may enable discovery biomarkers for immunity following or vaccination.
Introduction Zika virus (ZIKV) infection during pregnancy results in a spectrum of birth defects and neurodevelopmental deficits prenatally exposed infants, with no clear understanding why some pregnancies are more severely affected. Differential control maternal ZIKV may explain the adverse outcomes. Methods Here, we investigated whether magnitude breadth ZIKV-specific antibody response is associated better virologic using rhesus macaque model prenatal infection. We inoculated 18 dams an...
The Amazing AI Race: Pit Stops, Detours, and Red FlagsAbstractAcross North America, the National Association of Sewer Service Companies (NASSCO) has pioneered development a standardized observation defect coding system for underground infrastructure systems. Using NASSCO's Pipeline Assessment Certification Program (PACP), inspections can be evaluated in consistent defensible manner. Standardization serves as cornerstone establishing benchmarks comprehensive guidelines assessing pipe's...
The Amazing AI Race: Pit Stops, Detours, and Green FlagsAbstractAcross North America, the National Association of Sewer Service Companies (NASSCO) has pioneered development a standardized observation defect coding system for underground infrastructure systems. Using NASSCO's Pipeline Assessment Certification Program (PACP), inspections can be evaluated in consistent defensible manner. Standardization serves as cornerstone establishing benchmarks comprehensive guidelines assessing pipe's...
A Correction to this paper has been published: https://doi.org/10.1038/s42003-021-01866-z
Application of Metal-Bearing Seal Roller-Cone Bit Reduces Rig Time/Drilling Costs in Green Canyon, Deep Water Gulf Mexico Scott A. McLeod; McLeod Chevron Corp. Search for other works by this author on: This Site Google Scholar Thomas T. O'Grady; O'Grady Hughes Christensen Eric C. Sullivan; Sullivan John S. Mason; Mason Baker INTEQ Lin Chih Paper presented at the IADC/SPE Drilling Conference, New Orleans, Louisiana, February 2000. Number: SPE-59109-MS https://doi.org/10.2118/59109-MS...
Let $\mathcal{C}$ be a family of edge-colored graphs. A $t$-edge colored graph $G$ is $(\mathcal{C}, t)$-saturated if does not contain any in but the addition edge color $[t]$ creates copy some $\mathcal{C}$. Similarly to classical saturation functions, define $\mathrm{sat}_t(n, \mathcal{C})$ minimum number edges $(\mathcal{C},t)$ saturated graph. $\mathcal{C}_r(H)$ consisting every $H$ which uses exactly $r$ colors. In this paper we consider variety problems. We determine order magnitude...
This poster presents an innovative model for patient disease identification from clinical notes. CLSTM-Attention leverages the rich context information and learn features automatically to extract of patients. Preliminary evaluation verified effectiveness approach.
Abstract Cancer is a result of number genetic alterations that disturb normal, controlled cell growth and differentiation. Mutational events leading to the activation oncogenes or inactivation tumor-suppressor genes have been linked causally formation tumors. p53 one most important regulators transcription, cellular cycle, DNA repair apoptosis detected date. Anti-p53 antibodies in serum cancer patients. This immune response probably due self-immunization process strong immunogenicity...
Given graphs $H$ and $F$, a subgraph $G\subseteq H$ is an $F$-saturated of if $F\nsubseteq G$, but $F\subseteq G+e$ for all $e\in E(H)\setminus E(G)$. The saturation number $F$ in $H$, denoted $\text{sat}(H,F)$, the minimum edges $H$. In this paper we study numbers tripartite graphs. For $\ell\ge 1$ $n_1$, $n_2$, $n_3$ sufficiently large, determine $\text{sat}(K_{n_1,n_2,n_3},K_{\ell,\ell,\ell})$ $\text{sat}(K_{n_1,n_2,n_3},K_{\ell,\ell,\ell-1})$ exactly...