A5028786921- Network Security and Intrusion Detection
- CRISPR and Genetic Engineering
- Advanced Malware Detection Techniques
- Anomaly Detection Techniques and Applications
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Hippo pathway signaling and YAP/TAZ
- Immune Cell Function and Interaction
- Information and Cyber Security
- Hepatitis C virus research
- Internet Traffic Analysis and Secure E-voting
- Spam and Phishing Detection
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Digital and Cyber Forensics
- Microbial bioremediation and biosurfactants
- Artificial Intelligence in Games
- Photoacoustic and Ultrasonic Imaging
- Video Analysis and Summarization
- Ultrasound and Hyperthermia Applications
- Inflammation biomarkers and pathways
- Poxvirus research and outbreaks
- Ultrasound Imaging and Elastography
- Software System Performance and Reliability
- Barrier Structure and Function Studies
The University of Texas at El Paso
2023-2025
Zhongshan Hospital of Xiamen University
2024-2025
Chinese Academy of Sciences
2006-2024
Xiamen University
2019-2024
Institute of Information Engineering
2017-2024
Xian Yang Central Hospital
2024
West China Hospital of Sichuan University
2024
Sichuan University
2024
China Three Gorges University
2024
Land Consolidation and Rehabilitation Center
2023
Abstract Triggering receptor expressed on myeloid cells 2 (TREM2) is a microglial surface genetically linked to the risk for Alzheimer’s disease (AD). A proteolytic product, soluble TREM2 (sTREM2), abundant in cerebrospinal fluid and its levels positively correlate with neuronal injury markers. To gain insights into pathological roles of sTREM2, we studied sTREM2 brain 5xFAD mice, model AD, by direct stereotaxic injection recombinant protein or adeno-associated virus (AAV)-mediated...
Botnets are groups of compromised computers that botmasters (botherders) use to launch attacks over the Internet. To avoid detection, botnets DNS fast flux change mapping between IP addresses and domain names periodically. Domain generation algorithms (DGAs) employed generate a large number names. Detection techniques have been proposed identify malicious generated by DGAs. Three metrics, Kullback-Leibler (KL) distance, Edit distance (ED), Jaccard index (JI), used detect botnet domains with...
Chamomile has long been used as a medicinal plant due to its antioxidative and anti-inflammatory activity. Apigenin-7-O-glucoside (AG) is one of the major ethanol extract components from chamomile; however, underlying mechanism remains unclear.In this study, antioxidant potential activities AG were analysed compared with those trolox. We demonstrate protective effects on free radical-induced oxidative damage DNA, proteins erythrocytes. Flow cytometry assay was detect ROS production....
Upon encountering antigens, B cells may undergo multiple differentiation paths, including becoming plasma and memory cells. Although it is well-known that transcription factors govern gene expression programs underpinning these fate decisions in transcriptional level, the role of post-transcriptional regulators, with a focus on RNA-binding proteins, determination are lesser known. Here we find by RNA interactome capture-coupled CRISPR/Cas9 functional screening Csde1-Strap complex plays an...
Kinesin-5 s are bipolar motor proteins that contribute to cell division by crosslinking and sliding apart antiparallel microtubules inside the mitotic spindle. However, mechanism underlying interactions between kinesin-5 microtubule remains poorly understood. In this study, we investigated binding of BimC, a from Aspergillus nidulans, using combination total internal reflection fluorescence (TIRF) microscopy molecular dynamics (MD) simulations. TIRF experiments revealed increasing...
T helper 2 (Th2) cells orchestrate immunity against parasite infection and promote tissue repair but pathology in asthma fibrosis. Here, we examined the mechanisms driving pathogenic differentiation of Th2 cells. Single-cell analyses CD4+ from chronic rhinosinusitis patients revealed high expression hypoxia-inducible factor (HIF)2α In mice, HIF2α deficiency impaired alleviated asthmatic inflammation. lineage tracing approaches delineated a trajectory TCF1+Ly108+ stem-like to ST2+CD25+...
Abstract Purpose: Lethal giant larvae functions as a cell polarity regulator and tumor suppressor in Drosophila. Its evolutionary conservation implies role for its human homologue, Hugl-1. The aims of this study were to characterize Hugl-1 determine the clinical significance alterations hepatocellular carcinoma (HCC). Experimental Design: Sequence from 80 HCC specimens 5 lines characterized by reverse transcription-PCR sequence analysis. Western blot was used determining expression....
Loss-of-function mutations in sorting nexin 14 (SNX14) cause autosomal recessive spinocerebellar ataxia 20, which is a form of early-onset cerebellar that lacks molecular mechanisms and mouse models. We generated Snx14-deficient models observed severe motor deficits cell-autonomous Purkinje cell degeneration. SNX14 deficiency disrupted microtubule organization mitochondrial transport axons by destabilizing the microtubule-severing enzyme spastin, implicated dominant hereditary spastic...
With the increasing complexity of network, current network traffic has strong nonlinearity and burstiness. Therefore, traditional prediction model is no longer applicable. The neural model, especially LSTM, can well fit time-series data preserve information memory past. However, as for periodicity long-term span data, does not perform well. Based on this, this paper proposes LTS-TP (Long-Term Span Traffic Prediction model), a to solve problem. First, decomposes collected using improved STL...
The binding of insulin to its receptor triggers a signaling cascade regulated by protein complexes via tyrosine phosphorylation events on multitude associated proteins. To search novel phosphotyrosine proteins or involved in pathway, we employed method which Rat1 cells stably expressing the human were stimulated with without and sub-fractionated prior enrichment immunoprecipitation analysis LC−MS/MS. Bioinformatic manual confirmation peptide site assignments led identification 35 sites...
Cdc42, a member of the Rho guanosine triphosphatase (GTPase) family, plays important roles in regulation cytoskeleton, cell proliferation, polarity, and cellular transport, but little is known about its specific function mammalian liver. We investigated Cdc42 regulating liver regeneration. Using mouse model with liver-specific knockout (Cdc42LK), we studied regeneration after partial hepatectomy. Histological analysis, immunostaining, western blot analysis were performed to characterize...
Targeted modulation of transcription is necessary for understanding complex gene networks and has great potential medical industrial applications. CRISPR emerging as a powerful system targeted genome activation repression, in addition to its use editing. This protocol describes how design, construct, experimentally validate the function sequence-specific single guide RNAs (sgRNAs) repression (CRISPRi) or (CRISPRa) mammalian cells. In this technology, CRISPR-associated protein Cas9...