A5045938797- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- MicroRNA in disease regulation
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Synthesis and biological activity
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Quinazolinone synthesis and applications
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer-related Molecular Pathways
- Aldose Reductase and Taurine
- RNA Interference and Gene Delivery
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Metabolism, Diabetes, and Cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related gene regulation
- Occupational and environmental lung diseases
- Genetic factors in colorectal cancer
- Chronic Myeloid Leukemia Treatments
- Heat shock proteins research
University of Southern Denmark
2020-2024
Universitätsklinikum Erlangen
2017-2023
University of Turin
2006-2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2017-2022
Northwestern University
2012-2017
Thomas Jefferson National Accelerator Facility
2014
United States Army Medical Research and Development Command
2014
German Cancer Research Center
2010-2011
In-Q-Tel
2011
Heidelberg University
2010-2011
In patients with cancer, one of the main mechanism resistance to antimetabolite drugs is related higher levels thymidylate synthase (TS) activity.To investigate association between TS expression and histopathologic data, 56 resection specimens from nonsmall cell lung carcinoma (NSCLC) were collected consecutively. messenger RNA (mRNA) was evaluated in tumor by using real-time polymerase chain reaction (PCR) analysis; protein immunohistochemistry (IHC) formalin-fixed, paraffin-embedded (FFPE)...
The development of metastases is the main reason for cancer-related death in non-small cell lung cancer (NSCLC). initiation metastasis involves an increase motility mediated by loss cell-cell adhesion caused E-cadherin repression, a process commonly known as epithelial-to-mesenchymal transition. A role microRNA-200 family members regulating transition has recently been indicated but data about their expression tumors still unavailable. present study investigated miR-200c panel NSCLC lines (n...
MicroRNAs (miRNAs) are small non-coding RNAs which regulate gene expression by base-pairing to the 3'-UTR of target mRNA. Recently, miRNAs have been shown cancer metastasis, however, central molecular mechanisms this ability still need be investigated. Epithelial mesenchymal transition (EMT), is characterized especially repression E-cadherin and increased cell motility, an essential component metastasis progression. In present study, we found that Snai1, a known transcriptional repressor...
Purpose The relationship between thymidylate synthase (TS) expression and outcome in patients with malignant pleural mesothelioma (MPM) treated pemetrexed (P) was retrospectively evaluated. Patients Methods Sixty histologically confirmed MPM previously P platinum (45 of 60) or as single agent (15 were considered. Eighty-one control not P-treated also TS excision repair cross-complementation group 1 (ERCC1) gene levels evaluated by real-time polymerase chain reaction immunohistochemistry...
Cancer cells alter their metabolism to support malignant properties. In this study, we report that the glucose-transforming polyol pathway (PP) gene aldo-keto-reductase-1-member-B1 (AKR1B1) strongly correlates with epithelial-to-mesenchymal transition (EMT). This association was confirmed in samples from lung cancer patients and an EMT-driven colon mouse model p53 deletion. vitro, mesenchymal-like showed increased AKR1B1 levels, knockdown sufficient revert EMT. An equivalent level of EMT...
Highlights•Stimulation of CD95 induces cancer stemness through induction type I IFNs•Cancer stem cells express a STAT1-regulated gene signature•Radiation-resistant SCC and CD95-stimulated breast an IFN signature•Type IFNs stimulate cellsSummaryStimulation CD95/Fas drives maintains (CSCs). We now report that this involves activation signal transducer activator transcription 1 (STAT1) genes process is inhibited by active caspases. STAT1 enriched in CSCs cell lines, patient-derived human...
Abstract Purpose: The effect of translesion DNA synthesis system in conferring cellular tolerance to DNA-damaging agents has been recently described. polymerase η (Pol η) is part this machinery and vitro models showed that it can overcome damages caused by cisplatin UV rays. aim the present study was investigate role Pol mRNA expression levels non–small cell lung cancer (NSCLC). Experimental Design: were evaluated real-time PCR (a) formalin-fixed paraffin-embedded biopsies 72 NSCLC patients...
CD95 (Fas/APO-1), when bound by its cognate ligand CD95L, induces cells to die apoptosis. We now show that elimination of or CD95L results in a form cell death is independent caspase-8, RIPK1/MLKL, and p53, not inhibited Bcl-xL expression, preferentially affects cancer cells. All tumors formed mouse models low-grade serous ovarian chemically induced liver with tissue-specific deletion still expressed CD95, suggesting cannot the absence CD95. Death CD95R/L (DICE) characterized an increase...
Abstract Purpose: The predictive role of the quantification thymidylate synthase (TS) in tumors treated with antifolate drugs, such as 5-fluorouracil (5-FU), has been extensively reported a variety human tumors. Neuroendocrine (NET) represent potential targets agents, but no data on TS expression level these are currently available. Experimental Design: A series 116 NETs were collected, including 58 gastroenteropancreatic (GEP) and lung NETs. In 24 well-differentiated GEP neuroendocrine...
PURPOSE: In non-small cell lung cancer, higher thymidylate synthase (TS) levels have been reported in squamous carcinoma (SCC) compared with adenocarcinoma (ADC). Data on TS expression large-cell (LCC) are scanty. EXPERIMENTAL DESIGN: mRNA and protein were analyzed 42 surgical cases of pulmonary LCC, including 8 neuroendocrine carcinomas, controls represented by ADC (n = 41), SCC 30), small-cell (SCLC; n 33). also correlated the Ki67 E2F1. Moreover, reliability analysis was assessed 22...
Cancer cells frequently boost nucleotide metabolism (NM) to support their increased proliferation, but the consequences of elevated NM on tumor de-differentiation are mostly unexplored. Here, we identified a role for thymidylate synthase (TS), enzyme and established drug target, in cancer cell investigated its clinical significance breast (BC). In vitro, TS knockdown population CD24+ differentiated cells, attenuated migration sphere-formation. RNA-seq profiling indicated repression...
The identification of novel targets is paramount importance to develop more effective drugs and improve the treatment non-small cell lung cancer (NSCLC), leading cause cancer-related deaths worldwide. Since cells alter their metabolic rewiring during tumorigenesis along progression, targeting key players metabolism-associated proteins represents a valuable approach with high therapeutic potential. Metabolic fitness relies on functionality heat shock (HSPs), molecular chaperones that...
Genes involved in DNA repair and replication have been recently investigated as predictive markers of response to chemotherapy non-small cell lung cancer (NSCLC). However, few data on the expression these genes tumor compared with corresponding normal are available. The aim this study was evaluate differential mRNA levels 22 five different pathways: direct, base excision, nucleotide excision (NER), double-strand break (DSBR), postreplicative repair. In addition, six (REP) three telomere...
Aurora kinase A (AURKA) is a member of serine/threonine family. Several kinases belonging to this family are activated in the G2/M phase cell cycle being involved mitotic chromosomal segregation. AURKA overexpression significantly associated with neoplastic transformation several tumors and deregulated Kinases expression leads chromosome instability, thus contributing cancer progression. The purpose present study was investigate non small lung (NSCLC) specimens correlate its mRNA or protein...
Treatment failure in solid tumors occurs due to the survival of specific subpopulations cells that possess tumor-initiating (TIC) phenotypes. Studies have implicated G protein-coupled-receptors (GPCRs) cancer progression and acquisition TIC Many GPCRs signal through protein GNA13. In this study, we demonstrate GNA13 is upregulated many impacts metastases patients. levels modulate drug resistance TIC-like phenotypes patient-derived head neck squamous cell carcinoma (HNSCC) vitro vivo....