A5080705201- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Respiratory and Cough-Related Research
- Inhalation and Respiratory Drug Delivery
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Advanced biosensing and bioanalysis techniques
- Gastrointestinal Bleeding Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Mast cells and histamine
- Food Allergy and Anaphylaxis Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Eosinophilic Esophagitis
- Pediatric health and respiratory diseases
- Molecular Junctions and Nanostructures
- Platelet Disorders and Treatments
- RNA Research and Splicing
- Hepatitis Viruses Studies and Epidemiology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Case Reports on Hematomas
- Hepatitis B Virus Studies
- Pharmaceutical studies and practices
University of Rochester
2016-2022
McMaster University
2009-2019
University of Rochester Medical Center
2015
Sandwell & West Birmingham Hospitals NHS Trust
2012-2013
Sainsbury's (United Kingdom)
2013
St. Joseph’s Healthcare Hamilton
2002-2011
St. Joseph's Hospital
2002-2011
Research Institute of Human Morphology
2011
Carnegie Mellon University
2005-2009
University of Pittsburgh
2008-2009
Exercise is a common stimulus of bronchoconstriction in subjects with asthma, who also have after inhaling the sulfidopeptide leukotriene D4 (LTD4). The purpose this study was to investigate importance LTD4 as mediator exercise-induced bronchoconstriction.
Extensive evidence in animal models supports a role for IL-13 the pathobiology of asthma. IMA-638 and IMA-026 are fully humanized IgG(1) antibodies that bind to different epitopes neutralize bioactivity.We hypothesized anti-IL-13 treatment would inhibit allergen-induced late-phase asthmatic responses, airway hyperresponsiveness, inflammation subjects with asthma.Fifty-six mild, atopic asthma were recruited two double-blind, randomized, placebo-controlled, parallel group trials compare...
Allergen inhalation causes airway inflammation and an increase in histamine responsiveness. We have used cell counts sputum induced by hypertonic saline aerosol to assess before 32 h after asthmatic responses allergen. Twelve subjects (mean age, 27.4 yr; range, 20–38 yr) had test with D. farinae, ragweed pollen, or cat extract. All of them developed early response a fall FEV1 24.8% (SD, 6.3%); nine 12 definite late (fall ⩾ 15%), 10 responsiveness at (PC20 reduced greater than twofold)....
The drug product TPI ASM8 contains two modified phosphorothioate antisense oligonucleotides designed to inhibit allergic inflammation by down-regulating human CCR3 and the common beta chain (beta(c)) of IL-3, IL-5, granulocyte-macrophage colony-stimulating factor receptors.This study examined effects inhaled on sputum cellular influx, beta(c) mRNA protein levels, airway physiologic response after allergen.Seventeen subjects with mild atopic asthma were randomized in a crossover inhale 1,500...
Abstract There is increasing interest in the roles of covalently modified nucleotides RNA. has been, however, an inability to account for modifications secondary structure prediction because a lack software and thermodynamic parameters. We report solution these issues N 6 -methyladenosine (m A), allowing alphabet A, C, G, U, m A. The RNAstructure now works with user-defined nucleotide alphabets any size. also set nearest neighbor parameters helices loops containing using experiments....
Treatment with inhaled β2-agonists immediately before allergen inhalation inhibits allergen-induced early, but not late asthmatic responses (LAR). By contrast, 2 wk treatment albuterol increases airway to allergen. We examined the effects of regular on in inflammatory cells blood and induced sputum. Ten mild, stable allergic asthmatics (800 μ g/day) or placebo for 7 d a controlled, randomized, double-blind, crossover study. Allergen was performed 12 h after final dose. Methacholine...
Allergen inhalation by sensitized subjects results in acute bronchoconstriction, which can be followed a later bronchoconstrictor response, allergen-induced airway hyperresponsiveness, and increases inflammatory cells. Treatment with inhaled glucocorticosteroids attenuates asthmatic responses. The purpose of this study was to determine whether 1-wk pretreatment budesonide influences changes cells blood induced sputum. Seven mild atopic asthma were treated double-blind, placebo-controlled,...
The effect of regular inhaled beta-agonist on the treatment exercise-induced bronchoconstriction was studied. Eight subjects with took 1 week each salbutamol 200 microg qid or placebo in a random-order, double-blind, crossover study. They then withheld this for 8 hours before performing dry-air, sub-maximal exercise challenge at work-rate previously shown to induce 15% fall forced expiratory volume second (FEV1). Five minutes after exercise, they 100, and 5-minute intervals. mean...
Phosphodiesterase 4 (PDE4) inhibitors increase intracellular cyclic adenosine monophosphate (cAMP), leading to regulation of inflammatory cell functions. Roflumilast is a potent and targeted PDE4 inhibitor. The objective this study was evaluate the effects roflumilast on bronchoconstriction, airway hyperresponsiveness (AHR), inflammation in mild asthmatic patients undergoing allergen inhalation challenge.25 subjects with allergic asthma were randomized oral 500 mcg or placebo, once daily for...
Metabolic changes occurring during coronary insufficiency were studied in ten dogs by progressively reducing the rate of blood flow to perfused left main artery, while maintaining aortic pressure constant. Seven animals exhibited a net myocardial lactate production at low flows, averaging 2.8 mg/min. The remaining showed decreased uptake which fell from an average 40 1.8% lowest flows. A rise atrial occurred coincident with alteration metabolism all animals. Calculated vascular resistance...
Inhaled corticosteroids are effective antiinflammatory therapy for asthma; however, they do not completely abolish allergen-induced airway inflammation. Leukotriene modifiers attenuate both early and late allergen responses have properties. We reasoned that treatment with budesonide montelukast in combination might provide greater effects than either drug alone, the purpose of this study was to compare montelukast, alone or combination, on outcome variables after inhalation. Ten subjects...
The hypothesis underlying the present study was that some of variability in symptom intensity seen during acute bronchoconstriction may result from varying intensities several stimuli yielding sensations can be identified by specific descriptive expressions (symptoms). A total 232 subjects inhaled methacholine doubling concentrations to a 20% decrease FEV(1), or 64 mg/ml. prevalence dyspnea, nonspecific discomfort associated with act breathing, and 10 expressions. Each rated Borg scale...
Background: The allergen‐induced early asthmatic response [provocation concentration (PC) 20 , the causing a 20% forced expiratory volume in 1 s (FEV) fall] depends on level of IgE sensitivity and degree nonallergic airway hyperresponsiveness (AHR) can be predicted from histamine PC allergen skin test endpoint. Objectives: We examined relationships between methacholine endpoint assessed accuracy both ‐based prediction (using methacholine) new equation. Methods: From 158 challenges, were...
Inhaled allergen challenge of subjects with atopic asthmatic increases bone marrow eosinophil progenitor cells. Interleukin-5 (IL-5) specifically induces growth and maturation eosinophils. This study examined the effect on number total CD3+ cells expressing IL-5 protein mRNA in asthma who developed either allergen-induced isolated early responses, or late responses (dual responders). At 24 hours after challenge, dual responders had significantly greater blood airway eosinophilia compared...
Thromboxane A2(TxA2) has been implicated in the pathogenesis of airway hyperresponsiveness. The effects inhaled TxA2 on human function have not studied because its short half-life. U46619 is a chemical that mimics TxA2. purpose this study was to evaluate and methacholine responsiveness. Airway responsiveness measured 19 subjects (13 asthmatic six normal) expressed as provocative concentration causing 20% fall FEV1 (PC20). On one day, alone inhaled. second inhaled, then 1 h later third...
In this unit, protocols are provided for predicting RNA secondary structure with the user-friendly RNAstructure desktop computer program and Web server. The minimum free energy a set of suboptimal structures similar energies predicted. Prediction high-affinity oligonucleotide binding sites to structured target is also presented. © 2016 by John Wiley & Sons, Inc.
BACKGROUND--Platelet activating factor (PAF) has been implicated in the pathogenesis of airway hyperresponsiveness asthma. The purpose this study was to evaluate effects a selective PAF antagonist (WEB 2086), given doses known antagonise inhaled human subjects, on allergen induced early and late asthmatic responses hyperresponsiveness. METHODS--Eight atopic, mildly subjects were studied during screening period two treatment periods. During an which they be sensitised response measured as...