Emulsion hydrogels are promising materials for encapsulating and stabilizing high amounts of hydrophobic essential oils in hydrophilic matrices. In this work, clove oil-loaded (CS/OP-C) synthesized by combining covalent physical cross-linking approaches. First, oil (CO) was emulsified stabilized a chitosan (CS) solution, which further hardened Schiff base with oxidized pullulan (OP). Second, the were subjected to freeze–thaw cycles and, as result, physically cross-linked polymeric walls....

The aim of this study was to evaluate the antibacterial activity coriander essential oil and its major constituent, linalool, in combination with antibiotics against Gram-positive (methicillin-susceptible methicillin-resistant Staphylococcus aureus, S. epidermidis) Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli). chemical composition analyzed by gas chromatography flame ionization mass spectrometry detection. oil, linalool their combinations were assessed broth...

In recent years, phytofunctionalized AgNPs have attracted great interest due to their remarkable biological activities. the present study, were synthesized using Abies alba and Pinus sylvestris bark extracts. The chemical profile of these extracts was analyzed by LC-HRMS/MS. As a first step, synthesis parameters (pH, AgNO3 concentration, ratio extract AgNO3, temperature, reaction time) optimized. characterized ATR-FTIR spectroscopy, DLS, SEM, EDX, TEM. Their antioxidant, cytotoxic,...

Background/Objectives: Even with improvements in surgical techniques and the application of appropriate antibiotic prophylaxis, wound infections are still major public health problems low- middle-income countries. This study proposes design new particulate polymeric matrices based on chitosan (CS) for controlled release Metronidazole (MTZ), order it to be used treatment Clostridium perfringens infections. Methods: The nanoparticles were prepared via inverse emulsion using tannic acid (TA)...

Silver nanoparticles synthesized using plant extracts as reducing and capping agents showed various biological activities. In the present study, colloidal silver nanoparticle solutions were produced from aqueous of Picea abies Pinus nigra bark. The phenolic profile bark was analyzed by liquid chromatography coupled to mass spectrometry. synthesis monitored UV-Vis spectroscopy measuring Surface Plasmon Resonance band. characterized attenuated total reflection Fourier transform infrared...

Abstract The present study evaluates the biosynthesis of AgNPs and AuNPs using aqueous ethanolic Geum urbanum L. rhizome extracts. biosynthesized metal nanoparticles (MNPs) were characterized UV-Vis spectroscopy, FTIR, DLS, SEM, EDX, TEM. spectra confirmed synthesis through peaks corresponding to surface plasmon effect metallic Ag (400–430 nm) Au (530–570 nm). FTIR analysis indicated that alcohols, phenols, proteins, carbohydrates from G. extracts composition are involved in MNPs synthesis....

Combination of antibiotics with natural products is a promising strategy for potentiating antibiotic activity and overcoming resistance. The purpose the present study was to investigate whether morusin kuwanon G, prenylated phenolics in Morus species, have ability enhance reverse resistance Staphylococcus aureus epidermidis. Commonly used (oxacillin, erythromycin, gentamicin, ciprofloxacin, tetracycline, clindamycin) were selected combination studies. Checkerboard time-kill assays potential...

Essential oil of Nepeta racemosa Lam. was extracted and characterized to determine its antimicrobial activity potential use in applications. The essential loaded on polyvinyl alcohol-pullulan films gels by optical microscopy, scanning electron UV-Vis spectroscopy before having capacities assessed. from using gas chromatography coupled with mass spectroscopy, which indicated that the most abundant component nepetalic acid (55.5%), followed eucalyptol (10.7%) other compounds concentrations...

This study investigated the effects of incorporating grape pomace (GP) into fish diets on growth performance, physiological parameters, and biochemical composition carp (Cyprinus carpio L.). A total 180 carp, with an average initial weight 65 g, were reared in a recirculating aquaculture system (RAS). They divided control group (C), fed diet containing 0% GP, two experimental groups (V1 V2), 5% 10% respectively. The experiment lasted for eight weeks. Results revealed that most parameters not...

Feline and canine coronaviruses (FCoV CCoV, respectively) are common pathogens of cats dogs sometimes leading to lethal infections named feline infectious peritonitis (FIP) pantropic coronavirus infection. FCoV CCoV each subdivided into two serotypes, FCoV-I/II CCoV-I/II. A phylogenetic relationship is evident between, on one hand, CCoV-I/FCoV-I, the other CCoV-II/FCoV-II, suggesting that interspecies transmission can occur. The aim present study was evaluate prevalence (CoV)-infected...

In this study, the potential use of Artemisia dracunculus essential oil in bio-applications was investigated. Firstly, phytochemicals from were analyzed by different methods. Secondly, incorporated into hydrogel matrix based on poly(vinyl alcohol) (PVA) and agar (A). The structural, morphological, physical properties loaded with amounts thoroughly FTIR analysis revealed successful loading PVA/A matrix. influence mechanical antimicrobial activity also assessed. (EO dracunculus) tested using...

This study investigates the impact of including sorghum in diet common carp (Cyprinus carpio) on its growth, blood parameters, meat composition, intestinal microbiota, and oxidative stress. Experimental diets with varying content (0%—V0 or control, 10%—V1, 20%—V2, 30%—V3) were administered to weighing 43 g initially. Notably, 30% variant, entirely replaced corn barley diet. Chemical analysis unveiled a protein 14% fat 3.9%. Sorghum inclusion led decline final body weight gain, particularly...

The neurohormone oxytocin is known to exert a special function in the regulation of social relationships throughout vertebrate taxa. Recently it has been discovered that not only within-species, but certain cases, between-species bonds are also mediated by same hormone, e.g. case dog�human relationship. However, despite exponential growth findings at behavioural level, there still lot controversies on biochemical assessment canine samples. Thus, current study we replicate previous serum...

Lately there are increased evidences that oxytocin may be an important mediator in several psychiatric disorders including depression and anxiety. Oxytocin system seems to linked with neurotransmitters disorder, but also the hypothalamic�pituitary�adrenal (HPA) axis is a key factor stress regulation mechanisms. Considering receptor pattern of distribution both central peripherally growing data concerning disbalance we speculate at core psychosomatic disturbances. Also, recent brought...

Caprine arthritis encephalitis is an important disease of dairy goats with a worldwide distribution. Despite the eradication programs, this still prevalent and causing huge financial losses due to reduced lactation performance culling. The aim study was reveal presence CAEV antibodies among goat population from North-Eastern Romania. During 2014-2016, total 295 blood samples were collected healthy or diseased animals. serum tested for detection, using agar gel immunodiffusion (AGID) test....

The correlations between depression and stress are quite accepted nowadays, as well the fact that cortisol levels could also represent fundamental risk factors for major depressive disorder. However, when it comes to modifications of in depression, results controversial. Same goes irritable bowel syndrome (IBS), which is a functional digestive disorder characterized by strong biopsychological component, being highly influenced or exacerbated stressful stimuli. Thus, present report we were...

This article reports on a new way of valorizing vine leaves waste as renewable resource polyphenols. The nanoparticles containing zinc complexes were prepared by green synthesis method using the aqueous extract natural source ligands for complexation ions. characterized UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) in conjunction with energy dispersive X-ray (EDX). Another objective this study was to obtain cream into which...

Coronaviruses are widespread in mammals and birds, causing mostly digestive respiratory problems. In cats, feline coronaviruses undergo mutations while replicating, giving rise to the fatal coronavirus infectious peritonitis (FIP) disease. Several viral genes, among them 3c M, involved development of FIP. order study these shifts, samples 43 organs, feces, ascites collected from cats showing clinical signs were tested, sequences obtained for M genes analyzed. The gene nucleotides showed...

Mesenchymal stem cells (MSCs) can be obtained from different sources, such as adipose tissue, placenta, umbilical cord, etc., however, those the bone marrow are main cell source used in tissue repair, engineering and cell-based gene therapy. The methods of harvesting vary according to specie. Usually, smaller subject, more difficult is number reduced. This paper describes a technique that we considered most reliable easy apply for isolation differentiation mesenchymal derived rat (BM-MSCs)....

Adipose-derived mesenchymal stem cells (ADMSCs) have gained attention as a potential treatment for canine osteoarthritis due to their regenerative properties and ability modulate inflammation. ADMSCs are harvested from adipose (fat) tissue, which is abundant easily accessible in dogs. The collection procedure minimally invasive compared other sources like bone marrow. These can differentiate into various cell types, including chondrocytes, essential cartilage repair. This may help...

Stem cell - based therapy can be used as an efficient alternative in human medicine, especially degenerative diseases that cannot controlled or treated with traditional tools. Lately, the same approach became very attractive for treatment of similar disorders different animal species. Compared to embryonic totipotent cells, mesenchymal stem cells (MSCs) are more available isolation from multiple sources, most time stroma bone marrow adipose tissue provides a relatively abundant supply. Even...

In veterinary practice, otitis is the most commonly encountered condition in pet carnivores. Contributing factors and polyfactorial etiopathogenesis frequently lead to therapeutic failure medical interventions. Using a limited range of topical medicinal products intended for these conditions inevitably results resistance active substances. Our study, conducted on otic exudates (n=100) collected from dogs cats, highlighted complex polymicrobial etiology decrease sensitivity antimicrobials,...