A5060004974- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Effects of Environmental Stressors on Livestock
- Reproductive Physiology in Livestock
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Milk Quality and Mastitis in Dairy Cows
- Parkinson's Disease Mechanisms and Treatments
- Cancer-related gene regulation
- Genetic Mapping and Diversity in Plants and Animals
- Animal Nutrition and Physiology
- Meat and Animal Product Quality
- Medical Imaging Techniques and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Genetics and Reproduction
- Image and Object Detection Techniques
- X-ray Diffraction in Crystallography
- Breast Lesions and Carcinomas
- RNA regulation and disease
- Advanced X-ray Imaging Techniques
- Parkinson's Disease and Spinal Disorders
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- Protein Hydrolysis and Bioactive Peptides
Ningxia University
2020-2024
Northwest A&F University
2022
Sun Yat-sen Memorial Hospital
2019
Sun Yat-sen University
2019
Peking University Shenzhen Hospital
2019
Sun Yat-sen University Cancer Center
2019
Henry M. Jackson Foundation
2016
Windber Research Institute
2016
Thomas Jefferson University
2016
Walter Reed National Military Medical Center
2016
Long noncoding RNAs (lncRNAs) play an important regulatory role in various biological processes as a key factor. However, the complete expression profile of lncRNAs dairy cows and its function milk fat synthesis are unknown. In this study, RNA sequencing (RNA-seq) was used to research whole genome mRNA transcripts high low percentage (MFP) bovine mammary epithelial cells (BMECs), joint analysis carried out. We identified total 47 differentially expressed genes (DEGs) 38 (DELs, Padj <0.05),...
Abstract Low‐coverage whole genome sequencing (lcWGS) has great potential to effectively genotype large‐scale population and provide solid data for imputation; however, the time imputation needs be optimized. There is also no publicly available reference panel selection in cattle. Here, we proposed a combination of Beagle v5.4 phasing GLIMPSE2 imputation, which fast accurate cattle lcWGS data. Furthermore, established multi‐breed with 61.8 million SNPs based on 2976 worldwide cattle, 1766...
The objective of this study was to explore the genetic parameters conformation traits and milk production in Chinese Holstein cattle provide a reference for dairy breeding. We collected phenotypic data 23 five Holsteins used animal models estimate traits. estimated heritability ranged from 0.11 (angularity) 0.37 (heel depth) correlation between -0.73 (bone quality rear leg-rear view) 0.76 (chest width loin strength). 0.23 (somatic cell score) 0.50 (305-d yield). values -0.56 depth 305-d...
The Wnt family features conserved glycoproteins that play roles in tissue regeneration, animal development and cell proliferation differentiation. For its functional diversity importance, this has been studied several species, but not the Bovinae. Herein we identified 19 genes cattle, seven other species of Bovinae, described their corresponding protein properties. Phylogenetic analysis clustered 149 proteins 38 from human mouse into 12 major clades. same subfamilies shared similar motif...
The increase of longevity is intended to reduce involuntary culling rates, not extend the life span, and it reflects ability animals successfully cope with environment disease during production. Sire model, animal model repeatability models were used estimate (co) variance components fertility traits. Six thirteen traits analysed, including herd (HL), productive (PL), number days between first calving end lactation or (L1); second (L2); third (L3); fourth (L4); age at service, (AFC),...
Abstract Milk fat is the most important and energy-rich substance in milk, its content composition are reference elements evaluation of milk quality. However, current identification valuable candidate genes affecting limited. IlluminaPE150 was used to sequence bovine mammary epithelial cells (BMECs) with high low rates (MFP), weighted gene co-expression network (WGCNA) analyze mRNA expression profile data this study. As a result, total 10,310 were construct WGCNA, classified into 18 modules....
Fat deposition is an important economic trait in livestock and poultry production. However, the relationship between various genes signal pathways of fat still unclear to a large extent. The purpose this study analyze potential molecular targets related bovine subcutaneous adipose tissue.We downloaded GSE116775 microarray dataset from Gene Expression Omnibus (GEO). weighted gene co-expression network (WGCNA) was used expression profile, key modules with highest correlation tissue were...
In this study, we examined correlations between the deposition of inosine monophosphate (IMP) and mRNA expression adenylate kinase 1 (AK1) gene in Jingyuan chicken. The IMP content was determined by high-performance liquid chromatography. Transcriptome sequencing used to screen differentially expressed AK1 real-time quantitative polymerase chain reaction (PCR) determine level associated with synthesis. breast muscles both cocks hens found be significantly higher than that leg muscles....
Abstract Background : The objective of this study was to explore the genetic parameters conformation traits and milk production Chinese Holstein cattles in Northwest China, provide a reference for dairy cattle breeding region. phenotypic data 23 5 7,930 Holsteins from 18 pastures 2007 2020 were collected. With field, identification season, calving appraiser effect as fixed individual additive random effect, DMU package AIREML combined with EM algorithm used estimate traits. Results estimated...
Health traits have high economic values in dairy cattle breeding, which can cause considerable financial loss through involuntary culling. In this study, fourteen health were analyzed, including five composite traits: reproductive disorders (RD), udder (UH), digestive (DD), metabolic (MD), locomotory diseases (LD), and nine independent gestation peripartum (GDPD), irregular estrus cycle sterility (IECS), metritis (ME), mastitis (MA), abomasal displacement (AD), enteritis (EN), ketosis (KE),...
Intramuscular fat (IMF) content is an important economic factor in beef production. However, knowledge on the key factors controlling bovine IMF limited. In this study, using weighted gene co-expression network analysis (WGCNA), nine modules were identified and number of transcripts these ranged from 36 to 3191. Two found be significantly associated with deposition three genes (TCAP, MYH7, TNNC1) further by Protein-protein interaction (PPI), which may hub regulating deposition. addition,...
Objective: Inosine monophosphate (IMP) is a key factor that imparts of meat flavor. Differences in the IMP content muscles were evaluated to improve chicken quality.Methods: For this study, was detected by high performance liquid chromatography. The gene expression profiles Jingyuan chickens with different feeding patterns and sexes analyzed RNA-sequencing (RNA-seq).Results: Breast muscle free-range extremely significantly higher than caged (p<0.01). hens also cocks, but difference not...
Abstract Herd health is one of the key problems influencing efficiency dairy industry. Genetic selection, with a focus on animal health, important for herd improvement. This study aimed to estimate genetic parameters traits and their correlations fertility milk production in cattle. Based records from 58,549 lactating cows calved between 2015 2021, total 24 (six composite 18 independent traits), four five were analysed. First, linear threshold models used variance components heritabilities...
Each of the currently used helical reconstruction algorithms represents one tradeoff selection image noise or mA requirement and resultant artifacts versus width section profile. For many applications, performance tradeoffs different from those existed are desired. In this paper, we present a general framework incorporating longitudinal (z) filtration in CT reconstruction, which leads to new class with tunable characteristics suit various applications. The z-filtering algorithm enables us...
Abstract As key regulators, long non‐coding RNAs (lncRNAs) play a crucial role in the ruminant mammary gland. However, function of lncRNAs milk fat synthesis from dairy cows is largely unknown. In this study, we used weighted gene co‐expression network analysis (WGCNA) to comprehensive analyze expression profile data group's previous Illumina PE150 sequencing results based on bovine epithelial cells high‐ and low‐milk‐fat‐percentage (MFP) cows, identify core_lncRNAs significantly associated...
The proliferation and differentiation of pre-adipocytes are regulated by microRNAs (miRNAs) other factors. In this study, the potential functions bta-miR-6517 in regulation pre-adipocyte were explored. qRT-PCR, oil red O staining CCK-8 assay used to evaluate role bta-miR-6517. Further, target gene was identified using bioinformatics analysis, dual-luciferase reporter system qRT-PCR system. results found that overexpression promoted expression marker genes substantially increased adipocyte...
Substantive evidence has confirmed that nutrition state is associated with health risk and the onset of pubertal metabolic profile. Due to heterogeneity, adipose tissues in different anatomical positions tend show various mechanisms for nutrition. To date, complicated molecular early calf-hood on bovine tissue are still largely unknown. This study aimed identify key genes functionally enriched pathways visceral subcutaneous tissue.The RNA-seq data calves feeding low high dietary more than...
Flavour is an important factor in evaluating meat quality, and amino acids fats are components affecting flavour. In this study, we evaluated the relationship between variation of lysine residue addition slaughter performance quality broilers, which decreased with residues but improved broilers. 10% was most beneficial for reducing feed cost improving quality. Meanwhile, plasma metabolites broilers fed increasing concentrations supplemented feeds were analysed using liquid...
Abstract Milk production traits as the most important economic of dairy cows, they directly reflect benefits breeding and pasture. In this study, A disintegrin metalloproteinase‐12 ( ADAM12 ), Parkinson's disease gene 2 PRKN ) dipeptidyl peptidase‐like protein subtype 6 DPP6 polymorphism in 384 Chinese Holstein cows were detected by time‐of‐flight mass spectrometry through statistical analysis using software such Popgene 32, SAS 9.4 Origin 2022, relationship between single nucleotide...
Abstract BACKGROUND PhosphohistoneH3 (PPH3) is an emerging marker in breast cancer and has been linked to both patient survival age. Phosphorylation of HistoneH3 important step during the cell cycle leading proper compaction chromatin late G2 early mitosis. Here we assessed use PPH3 as a prognostic within group invasive cancers Clinical Breast Care Project (CBCP). METHODS CBCP participants their samples were collected following IRB-approved, HIPAA-compliant protocols. Samples from 157...