Justin Engelbert
A5107983109- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Neonatal Respiratory Health Research
- IL-33, ST2, and ILC Pathways
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Cutaneous lymphoproliferative disorders research
- Pregnancy and preeclampsia studies
- Hematopoietic Stem Cell Transplantation
- COVID-19 Clinical Research Studies
- Lymphoma Diagnosis and Treatment
- Ear Surgery and Otitis Media
- Ear and Head Tumors
- MicroRNA in disease regulation
- Inflammatory Bowel Disease
- SARS-CoV-2 and COVID-19 Research
- Prenatal Screening and Diagnostics
- Acute Lymphoblastic Leukemia research
- Nasal Surgery and Airway Studies
Wellcome Sanger Institute
2023-2024
Newcastle University
2020-2023
Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T B lymphocyte antigen receptor analyses over 780,000 peripheral mononuclear from a cross-sectional cohort 130 patients with varying severities COVID-19. identified expansion nonclassical monocytes expressing complement transcripts (CD16
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult with atopic dermatitis psoriasis. leveraged these datasets to compare cell states across development, homeostasis, disease. Our analysis revealed an enrichment innate immune during first trimester clonal expansion disease-associated lymphocytes...
The extraembryonic yolk sac (YS) ensures delivery of nutritional support and oxygen to the developing embryo but remains ill-defined in humans. We therefore assembled a comprehensive multiomic reference human YS from 3 8 postconception weeks by integrating single-cell protein gene expression data. Beyond its recognized role as site hematopoiesis, we highlight roles metabolism, coagulation, vascular development, hematopoietic regulation. reconstructed emergence decline stem progenitor cells...
Abstract The gastrointestinal tract is a multi-organ system crucial for efficient nutrient uptake and barrier immunity. Advances in genomics surge diseases 1,2 has fuelled efforts to catalogue cells constituting tissues health disease 3 . Here we present systematic integration of 25 single-cell RNA sequencing datasets spanning the entire healthy development adulthood. We uniformly processed 385 samples from 189 controls using newly developed automated quality control approach (scAutoQC),...
Cutaneous T cell lymphoma (CTCL) is a potentially fatal clonal malignancy of cells primarily affecting the skin. The most common form CTCL, mycosis fungoides, can be difficult to diagnose, resulting in treatment delay. We performed single-cell and spatial transcriptomics analysis skin from patients with fungoides-type CTCL an integrated comparative human atlas datasets healthy inflamed revealed co-optation helper 2 (T
Abstract The human skin confers biophysical and immunological protection through a complex cellular network that is established early in development. We profiled ~500,000 single cells using RNA-sequencing from healthy adult developing skin, patients with atopic dermatitis psoriasis. Our findings reveal predominance of innate lymphoid macrophages contrast to T migratory dendritic skin. demonstrate dual keratinocyte differentiation trajectories activated circuits comprising vascular...
Otitis media is an umbrella term for middle ear inflammation; ranging from acute infection to chronic mucosal disease. It a leading cause of antimicrobial therapy prescriptions and surgery in children. Despite this, treatments have changed little over 50 years. Research has been limited by the lack physiological models epithelium.We develop novel human epithelial culture using air-liquid interface (ALI) system; akin healthy ventilated vivo. We validate this immunohistochemistry,...
Abstract Cutaneous T-cell lymphoma (CTCL) is a potentially fatal clonal malignancy of T cells primarily affecting the skin. The most common form CTCL, mycosis fungoides (MF), can be difficult to diagnose resulting in treatment delay. pathogenesis CTCL not fully understood due limited data from patient studies. We performed single-cell RNA sequencing and spatial transcriptomics profiling skin patients with MF-type an integrated comparative analysis human cell atlas datasets healthy skin,...
Abstract The yolk sac (YS) represents an evolutionarily-conserved extraembryonic structure that ensures timely delivery of nutritional support and oxygen to the developing embryo. However, YS remains ill-defined in humans. We therefore assemble a complete single cell 3D map human from 3-8 post conception weeks by integrating multiomic protein gene expression data. reveal as site primitive definitive haematopoiesis including YS-specific accelerated route macrophage production, source...
Abstract Throughout postnatal life, haematopoiesis in the bone marrow (BM) maintains blood and immune cell production. Haematopoiesis first emerges human BM at 12 post conception weeks while fetal liver (FL) is still expanding. Yet, almost nothing known about how evolves to meet highly specialised needs of fetus newborn infant. Here, we detail development including stroma using single RNA-sequencing. We find that full repertoire established a short time window 6-7 early second trimester....