A5055763104- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Cutaneous lymphoproliferative disorders research
- Long-Term Effects of COVID-19
- Biomedical Ethics and Regulation
- Tissue Engineering and Regenerative Medicine
- Viral Infections and Vectors
- Hair Growth and Disorders
- Phagocytosis and Immune Regulation
- Malaria Research and Control
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Dermatology and Skin Diseases
- Vector-borne infectious diseases
- Neonatal Respiratory Health Research
- Adenosine and Purinergic Signaling
Ludwig-Maximilians-Universität München
2019-2025
Wellcome Sanger Institute
2023-2024
Institute of Immunology
2021-2024
Urologische Klinik München
2022
Disease manifestations in COVID-19 range from mild to severe illness associated with a dysregulated innate immune response. Alterations function and regeneration of dendritic cells (DCs) monocytes may contribute immunopathology influence adaptive responses patients. We analyzed circulating DC monocyte subsets 65 hospitalized patients mild/moderate or disease acute recovery healthy controls. Persisting reduction all subpopulations was accompanied by an expansion proliferating Lineage-HLADR+...
Human prenatal skin is populated by innate immune cells, including macrophages, but whether they act solely in immunity or have additional functions morphogenesis unclear. Here we assembled a comprehensive multi-omics reference atlas of human (7-17 post-conception weeks), combining single-cell and spatial transcriptomics data, to characterize the microanatomical tissue niches skin. This revealed that crosstalk between non-immune cells underpins formation hair follicles, implicated scarless...
Abstract The yellow fever 17D vaccine (YF17D) is highly effective but frequently administered to individuals with pre-existing cross-reactive immunity, potentially impacting their immune responses. Here, we investigate the impact of flavivirus immunity induced by tick-borne encephalitis virus (TBEV) on response YF17D vaccination in 250 up 28 days post-vaccination (pv) and 22 sampled one-year pv. Our findings indicate that previous TBEV does not affect early IgM-driven neutralizing YF17D....
Highlights•Sex and CMV status affect baseline immune variation but not YF17D vaccine outcomes•High numbers of activated CD4+ T cells at link to stronger responses•Elevated CXCL10 levels are negatively associated with responses•Environmental factors contribute the tied responseSummaryThe live-attenuated yellow fever 17D (YF17D) is a model acute viral infection that induces long-lasting protective immunity. Among immunocompetent adults, responses vary significantly. To understand sources this...
Abstract Infection with SARS‐CoV‐2 is associated thromboinflammation, involving thrombotic and inflammatory responses, in many COVID‐19 patients. In addition, immune dysfunction occurs patients characterised by T cell exhaustion severe lymphopenia. We investigated the distribution of phosphatidylserine (PS), a marker dying cells, activated platelets platelet‐derived microparticles (PMP), during clinical course COVID‐19. found an unexpectedly high amount blood cells loaded PS + PMPs for weeks...
COVID-19, the disease caused by SARS-CoV-2 infection, can assume a highly variable course, ranging from asymptomatic which constitutes majority of cases, to severe respiratory failure. This implies diverse host immune response SARS-CoV-2. However, immunological underpinnings underlying these divergent courses remain elusive. We therefore set out longitudinally characterize signatures convalescent COVID-19 patients stratified according their severity. Our unique cohort consists 74 not...
Plasmacytoid and conventional dendritic cells (pDC cDC) are generated from progenitor in the bone marrow commitment to pDCs or cDC subtypes may occur earlier later stages. Cells within CD11c+MHCII-/loSiglec-H+CCR9lo DC precursor fraction of mouse generate both cDCs. Here we investigate heterogeneity subsets this compartment by single-cell transcriptomics high-dimensional flow cytometry combined with cell fate analysis: Within pool expressing high levels Ly6D lacking expression transcription...
Abstract Disease manifestations in COVID-19 range from mild to severe illness associated with a dysregulated innate immune response. Alterations function and regeneration of dendritic cells (DC) monocytes may contribute immunopathology influence adaptive responses patients. We analyzed circulating DC monocyte subsets 65 hospitalized patients mild/moderate or disease acute recovery healthy controls. Persisting reduction all subpopulations was accompanied by an expansion proliferating Lineage...
Abstract Human pathogenic flaviviruses pose a significant health concern and vaccination is the most effective instrument to control their circulation. How pre-existing immunity antigenically related viruses modulates immunization outcome remains poorly understood. In this study, we evaluated effect of against tick-borne encephalitis virus (TBEV) on epitope immunodominance immunogenicity yellow fever 17D vaccine (YF17D) in cohort 250 human vaccinees. Following YF17D vaccination, all study...
Abstract Cutaneous T-cell lymphoma (CTCL) is a potentially fatal clonal malignancy of T cells primarily affecting the skin. The most common form CTCL, mycosis fungoides (MF), can be difficult to diagnose resulting in treatment delay. pathogenesis CTCL not fully understood due limited data from patient studies. We performed single-cell RNA sequencing and spatial transcriptomics profiling skin patients with MF-type an integrated comparative analysis human cell atlas datasets healthy skin,...
Dysregulation of the myeloid cell compartment is a feature severe disease in hospitalized COVID-19 patients. Here, we investigated response circulating dendritic (DC) and monocyte subpopulations SARS-CoV-2 infected outpatients with mild compared it to healthy individuals yellow fever vaccine virus YF17D as model well-coordinated viral infection. In SARS-CoV-2-infected DCs were persistently reduced for several weeks whereas after vaccination DC numbers decreased temporarily rapidly...
Abstract Introduction and aims Molecular regulation of mammalian skin development has been largely derived from murine studies. The lack human studies stems primarily the difficulties in prenatal access. There is an important need to study owing notable differences between mouse paucity mechanistic understanding congenital disorders. Methods We assembled first comprehensive multiomic reference atlas (21 samples 7 16 postconception weeks), combining single-cell (235 201 cells) spatial...
Abstract Introduction and aims Hair-bearing skin organoids (SkOs) are a complex three-dimensional (3D) model that faithfully recapitulates human as multicellular organ with appendage structures. The development of such models provides unique potential for investigating translational dermatology. Although this answers many demands an investigative tool, one obvious limitation is the absence immune cells in SkOs. Our aim to generate coculture systems SkOs macrophages investigate how influence...
Abstract Yellow fever vaccination provides long-lasting protection and is a unique model for studying the immune response to an acute RNA virus infection in humans. To elucidate early innate events preceding rapid generation of protective immunity, we performed transcriptome analysis human blood dendritic cell (DC) monocyte subpopulations before 3, 7, 14 28 days after vaccination. We detected temporary upregulation IFN-stimulated genes (ISG) all DC subsets on day 3 7 as well type specific...
Cutaneous T cell lymphoma (CTCL) is a potentially fatal clonal malignancy of cells primarily affecting the skin. The most common form CTCL, mycosis fungoides, can be difficult to diagnose, resulting in treatment delay. We performed single-cell and spatial transcriptomics analysis skin from patients with fungoides-type CTCL an integrated comparative human atlas datasets healthy inflamed revealed co-optation helper 2 (T
Abstract Fibroblasts are critical cells that shape the architecture and cellular ecosystems in multiple tissues. Understanding fibroblast heterogeneity their spatial context health disease has enormous clinical relevance. In this study, we constructed a spatially-resolved atlas of human skin fibroblasts from healthy 23 disorders. We define 6 major populations further three disease-specific subtypes, demonstrate composition different types disease. characterise human-specific fibroblastic...
Summary Human prenatal skin is populated by innate immune cells including macrophages, and whether they act solely in immunity or have additional functions morphogenesis unclear. We assembled the first comprehensive multi-omic reference atlas of human (7-16 post-conception weeks), combining single cell spatial transcriptomic data, to characterise skin’s microenvironmental cellular organisation. This revealed that crosstalk between non-immune underpins formation hair follicles, has...
Abstract Colorectal cancer is one of the most common cancers and a major cause mortality. Proinflammatory antitumor immune responses play critical roles in colitis-associated colon cancer. CCL17, chemokine C-C family ligand for CCR4, expressed by intestinal dendritic cells steady state upregulated during colitis mouse models inflammatory bowel disease patients. In this study, we investigated expression pattern functional relevance CCL17 tumor development using CCL17–enhanced GFP-knockin...
SUMMARY Infection with SARS-CoV-2 is associated thromboinflammation, involving thrombotic and inflammatory responses, in many COVID-19 patients. In addition, immune dysfunction occurs patients characterized by T cell exhaustion severe lymphopenia. We investigated the distribution of phosphatidylserine (PS), a marker dying cells, activated platelets, platelet-derived microparticles (PMP), during clinical course COVID-19. found an unexpectedly high amount blood cells loaded PS + PMPs for weeks...