A5087798112- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Medical Imaging Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Atomic and Subatomic Physics Research
- Glioma Diagnosis and Treatment
- Immune Cell Function and Interaction
- NMR spectroscopy and applications
- Solid-state spectroscopy and crystallography
- Enzyme Structure and Function
- Lanthanide and Transition Metal Complexes
- MRI in cancer diagnosis
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Hepatocellular Carcinoma Treatment and Prognosis
- Magnetic and Electromagnetic Effects
- Hematopoietic Stem Cell Transplantation
- Radiomics and Machine Learning in Medical Imaging
- Cancer Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Cytomegalovirus and herpesvirus research
- Cancer-related Molecular Pathways
- Diet and metabolism studies
The University of Texas MD Anderson Cancer Center
2013-2022
The University of Texas Health Science Center at Houston
2017-2021
University Hospital Frankfurt
2016
Goethe University Frankfurt
2016
Genotypic and phenotypic alterations in the bone marrow (BM) microenvironment, particular osteoprogenitor cells, have been shown to support leukemogenesis. However, it is unclear how leukemia cells alter BM microenvironment create a hospitable niche. Here, we report that acute myeloid (AML) but not normal CD34+ or CD33+ induce osteogenic differentiation mesenchymal stromal (MSCs). In addition, AML inhibited adipogenic of MSCs. Mechanistic studies identified AML-derived BMPs activate Smad1/5...
To circumvent donor-to-donor heterogeneity which may lead to inconsistent results after treatment of acute graft-versus-host disease with mesenchymal stromal cells generated from single donors we developed a novel approach by generating these pooled bone marrow mononuclear 8 healthy "3rd-party" donors. Generated were frozen in 209 vials and designated as cell bank. These served source for generation clinical grade end-products, exhibited typical phenotype, trilineage differentiation...
Purpose To explore the effects of noise and error on kinetic analyses tumor metabolism using hyperpolarized [1‐ 13 C] pyruvate. Methods Numerical simulations were performed to systematically investigate noise, number unknowns, in parameter estimates analysis apparent rate chemical conversion from pyruvate lactate ( k PL ). A pharmacokinetic model with two physical pools spins was used generate analyze synthetic data. Results The reproducibility worsened quickly when peak signal‐to‐noise...
BackgroundA generation of therapies targeting tumor metabolism is becoming available for treating glioma. Hyperpolarized MRI uniquely suited to directly measure the metabolic effects these emerging treatments.PurposeTo explore feasibility use hyperpolarized [1-carbon 13 {13C}]-pyruvate real-time measurement and response treatment with a glycolytic inhibitor in an orthotopic mouse model glioma.Materials MethodsIn this animal study, anatomic dynamic 13C MR spectroscopy were performed at 7 T...
Magnetic resonance imaging of hyperpolarized pyruvate provides a new biomarker for cancer metabolism, based on the dynamic in vivo conversion to lactate. Methods quantification signal evolution need be robust and reproducible across range experimental conditions. Pharmacokinetic analysis spectroscopic data from its metabolites generally assumes that arises ideal rectangular slice excitation profiles. In this study, we examined whether assumption could lead bias kinetic and, if so, such can...
TP53 mutations occur in ∼50% of all human tumors, with increased frequency aggressive cancers that are notoriously difficult to treat. Additionally, p53 missense remarkably predictive refractoriness chemo/radiotherapy various malignancies. These observations have led the development mutant p53-targeting agents restore function. An important unknown is which p53-mutant tumors will respond reactivation-based therapies. Here, we found a heterogeneous impact on therapeutic response restoration,...
Purpose: The ability to produce precisely targeted beams as small 1 mm necessitates the understanding and management of intra-fraction motion. This study evaluated lung motion in free-breathing mice compared imaging (3D 4D reconstruction) breath-hold for use treatment planning. Methods: Five were imaged weekly six weeks using X-RAD 225Cx system. Each week, CBCT projections acquired during under anesthesia reconstructed into 3D images (6 phases). Superior-inferior, anterior-posterior,...
Dynamic contrast enhanced MRI was used to derive perfusion terms which were incorporated into the fitting of hyperpolarized pyruvate lactate apparent conversion rates. Incorporation DCE data reduced fit metabolic rates across set but more so for post therapy resulting in a significant decrease kpl after therapy. These results suggest that effort is needed disentangle from effects when analyzing HP data.
In order to track immune cells loaded with 19F compound, a nanoformulation based on iron-doped perfluoro-15-crown-5-ether, called Fe-PFCE was developed for Fluorine-19 MRI. It’s relaxation time characterized custom designed 8 channel Fluorine transmit/receive head coil clinical 3Tesla MR scanner. Its (T1/T2) 39ms/7ms as comparing 894ms/196ms original (undoped) “PFCE”. The SNR/19F-concentration ratio of 1ml measured by bSSFP found be 53% stronger than that PFCE. A good signal profile yielded...
Motivation: Changes in metabolic imaging biomarkers offer tremendous potential for assessing response and optimizing treatment patients with head neck squamous cell carcinoma (HNSCC). Goal(s): To assess the translational viability of MRI hyperpolarized (HP) pyruvate HNSCC. Approach: Patients HNSCC are recruited into a prospective study scanned HP [1-13C]-pyruvate test-retest paradigm before start therapy. Results: Preliminary data indicates that reproducibility semi-quantitative measures...
Motivation: Transmit gain calibration is necessary for nonproton hyperpolarized MRI to accurately calibrate excitation angles. Goal(s): To implement a phase-based TG method using the Bloch-Siegert shift HP on preclinical 7T scanner. Approach: An off-resonance Fermi-shaped pulse follows directly an pulse. Percent difference between our measured factors and validation 1H 13C were calculated. Results: Our allows fast, accurate, robust of studies with minimal error (<5%) 13C. Impact: This...
Abstract Purpose A specialized Helmholtz‐style 13 C volume transmit “clamshell” coil is currently being utilized for excitation in pre‐clinical and clinical hyperpolarized MRI studies aimed at probing the metabolic activity of tumors various target anatomy. Due to widespread use this clamshell design, it important that effects B 1 + profile on HP signal evolution quantification are well understood. The goal study was characterize field assess impact inhomogeneities semi‐quantitative...
Purpose Metabolic magnetic resonance imaging (MRI) using hyperpolarized [1‐ 13 C]‐pyruvate offers unprecedented new insight into disease and response to therapy. C‐enriched reference standards are required enable fast accurate calibration for C studies, but care must be taken ensure that the is compatible with both 1 H acquisitions. The goal of this study was optimize composition a C‐urea dual‐tuned C/ endorectal coil minimize artifacts in metabolic multiparametric MRI studies involving...
Pediatric brain tumors including medulloblastomas and ATRTs are associated with significant mortality morbidity. Much of this morbidity is due to conventional treatments: radiation chemotherapy. Therefore, there an urgent need develop new therapeutic options combat these devastating diseases. Immunotherapy has gained traction as a potential alternative current treatments. Because many immunotherapies rely on the presence tumor-associated antigens (TAAs) pediatric have poorly defined antigen...
Purpose: IGRT tools are most useful when registering similar datasets. In particular it is difficult to automatically register reference MR images with treatment CT or CBCT images. We proposed create a pseudo from the and CT. Methods: Two mice were sacrificed immobilized on rigid platform, then imaged small animal T1 T2 weighted MRI CBCT. Data first mouse was develop an atlas data 2nd validate system. To MRI, we established intensity conversion curve between MRI. This maps of fat tissue soft...
Metabolic imaging of hyperpolarized pyruvate can provide new insight into cancer progression and therapeutic response. Pharmacokinetic modeling be used to determine k PL , the apparent rate constant for conversion lactate. Quantitative evaluation signal evolution in vivo challenging due nonstationary multiple barriers between intravascular precursor its intracellular In this work, we evaluated using suspensions anaplastic thyroid prostate cells. A pharmacokinetic model with two physical...
Simulations were used to evaluate the effect of rapid vascular flow on spatial spectral pulses for hyperpolarized MRI. Simulation results show that effects reduced on-resonance excitation angle while increasing off-resonance in a velocity dependent manner. These impacts will be particularly important when trying estimate an arterial input function. Flow may also produce measurable lactate signal flowing pyruvate, was observed study thyroid.
Abstract Purpose To assess tumor tissue perfusion and vascular permeability through multiparametric functional imaging, to evaluate their correspondence with histopathologic parameters of necrosis hypoxia in a rat model hepatocellular carcinoma treated multikinase inhibitor. Materials Methods Rat hepatoma McA-RH7777 cells were implanted the left liver lobe nineteen male Buffalo rats. Exactly 2 weeks after inoculation, animals randomly assigned remain untreated (n=10) or receive daily dose...
Nonalcoholic Fatty Liver Disease (NAFLD) and Steatohepatitis (NASH) are increasingly prevalent in the United States. A diagnosis of NAFLD left untreated directly leads to increase NASH morbidity which is a result over‐nutrition, poor diet, lack exercise, sedentary lifestyle. However, typically reversible with change group common conditions that occur when excess fat accumulates liver those who consume little alcohol. Changes ketogenesis within has been associated development high‐fat diet...