A5100391970- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Fetal and Pediatric Neurological Disorders
- Parvovirus B19 Infection Studies
- Genomic variations and chromosomal abnormalities
- Ovarian function and disorders
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Assisted Reproductive Technology and Twin Pregnancy
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Sexual Differentiation and Disorders
- Reproductive System and Pregnancy
- Congenital Anomalies and Fetal Surgery
- Mitochondrial Function and Pathology
- Birth, Development, and Health
- Urological Disorders and Treatments
- Reproductive Health and Technologies
- Metabolism and Genetic Disorders
- Ectopic Pregnancy Diagnosis and Management
- RNA modifications and cancer
- Tumors and Oncological Cases
- Animal Genetics and Reproduction
Nanjing Agricultural University
2009-2024
Chinese PLA General Hospital
2015-2023
Hangzhou Children's Hospital
2023
First Affiliated Hospital of Xi'an Jiaotong University
2022
Xi'an Jiaotong University
2022
Huazhong University of Science and Technology
2022
Chinese Academy of Agricultural Sciences
2022
National Center for Clinical Laboratories
2014-2022
Nanjing Medical University
2017-2022
Hebei Medical University
2010-2022
To report the performance of massively parallel sequencing (MPS) based prenatal noninvasive fetal trisomy test on cell-free DNA from maternal plasma in a routine clinical setting China.The MPS-based was offered as screening for trisomies 21 and 18 to pregnant women 49 medical centers over 2 years. A total 11,263 participants were recruited performed 11,105 pregnancies. Fetal outcome data obtained after expected date confinement.One hundred ninety cases classified positive, including 143 47...
Abstract Background Conventional prenatal screening tests, such as maternal serum tests and ultrasound scan, have limited resolution accuracy. Methods We developed an advanced noninvasive diagnosis method based on massively parallel sequencing. The Noninvasive Fetal Trisomy (NIFTY) test, combines optimized Student’s t-test with a locally weighted polynomial regression binary hypotheses. applied the NIFTY test to 903 pregnancies compared diagnostic results those of full karyotyping. Results...
ABSTRACT Objective To report secondary or additional findings arising from introduction of non‐invasive prenatal testing (NIPT) for aneuploidy by whole genome sequencing as a clinical service. Methods Five cases with were reviewed. Results In Case 1, NIPT revealed large duplication in chromosome 18p, which was supported arrayCGH amniocyte DNA, final karyotype showing mosaic tetrasomy 18p. 2, deletion the proximal long arm 18 maternal origin suspected and confirmed white cell DNA. 3, negative...
Hydroxymethyl glutaryl-coenzyme A reductase degradation protein 1 (Hrd1) is an endoplasmic reticulum (ER)-transmembrane E3 ubiquitin ligase that has been studied in yeast, where it contributes to ER quality control by ER-associated (ERAD) of misfolded proteins accumulate during stress. Neither Hrd1 nor ERAD the heart, or cardiac myocytes, critical for proper heart function.The objective this study were elucidate roles stress, ERAD, and viability cultured myocytes mouse vivo.The effects small...
Next generation sequencing (NGS) is now being used for detecting chromosomal abnormalities in blastocyst trophectoderm (TE) cells from vitro fertilized embryos. However, few data are available regarding the clinical outcome, which provides vital reference further application of methodology. Here, we present a evaluation NGS-based preimplantation genetic diagnosis/screening (PGD/PGS) compared with single nucleotide polymorphism (SNP) array-based PGD/PGS as control. A total 395 couples...
Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies looked at cell treatment natural aging (NOA). We designed this experimental study investigate whether human amniotic mesenchymal (hAMSCs) retain restore function, and how hAMSCs work process. To build NOA mouse model, mice were fed for 12–14 months normally with young fertile female as normal control group (3–5 old). Hematoxylin eosin staining permitted...
ABSTRACT Objective The objective of this study is to assess the performance noninvasive prenatal testing for trisomies 21 and 18 on basis massively parallel sequencing cell‐free DNA from maternal plasma in twin pregnancies. Method A double‐blind was performed over 12 months. total 189 pregnant women carrying twins were recruited seven hospitals. Maternal detect 18. fetal karyotype used as gold standard estimate sensitivity specificity sequencing‐based test. Results There nine cases trisomy...
Many reports have shown that various kinds of stem cells the ability to recover premature ovarian aging (POA) function. Transplantation human amniotic epithelial (hAECs) improves function damaged by chemotherapy in a mice model. Understanding how evaluate distinct effects adult curing POA and choose clinical application is lacking. To build different degrees model, were administered doses cyclophosphamide: light dose (70 mg/kg, 2 weeks), medium 1 week; 120 week), high (120 weeks)....
Several studies have indicated that mutations of LARS2 are associated with premature ovarian insufficiency (POI). However, the pathogenic mechanism in POI has not been reported yet. In present study, expression levels and E2F1 granulosa cells (GCs) patients were examined. CCK-8 Edu assay performed to determine effect on cell proliferation. Apoptosis rate, mitochondrial membrane potential, reactive oxygen species (ROS), cytoplasm Ca2+ analyzed by flow cytometry. Western blot was conducted...
Non-invasive prenatal screening for fetal Down syndrome (NIFTY) by maternal plasma sequencing was performed in 12 subjects with twin pregnancies, including 11 normal fetuses and 1 discordant Trisomy 21. For every sample, it processed, sequenced reported as soon collected other clinical samples singleton pregnancies. The NIFTY test negative the pregnancies carried fetuses, positive (high risk) case sensitivity specificity were both 100%. This small series suggested a 21 is feasible
Abstract Objective The objective of the study is to report performance noninvasive prenatal testing (NIPT) in twin pregnancies after treatment assisted reproductive technology (ART). Method In two years period, 565 pregnant women with ART were prospectively tested by NIPT for screening trisomy 21 (T21), 18 (T18), and 13 (T13) sequencing cell‐free DNA maternal plasma. Positive results confirmed karyotyping, while negative interviewed delivery. Pregnant decision based on confirmation was...
Non-invasive prenatal testing (NIPT) has been confirmed as the most accurate screening test for trisomies 21, 18, and 13. However, reports on NIPT performance in sex chromosome aneuploidies (SCA) based real clinical data are still limited. High-throughput massively parallel genomic sequencing (MPS) technique was used to screen fetal SCAs part of research determine potential value detecting second trimester. A number 12,243 consecutive cases from a single center were included this study. The...
Demyelination in vascular dementia (VD) is partly attributable to inflammation induced by chronic cerebral hypoperfusion (CCH). Remyelination contributes the recovery of cognitive impairment inducing proliferation and differentiation oligodendrocyte progenitor cells. It was previously reported that Dl-3-n-butylphthalide (NBP) promotes improvement. However, whether NBP can stimulate remyelination suppress after CCH remains unclear. To answer this question, present study investigated effects...
Background Brain-derived neurotropic factor (BDNF) was originally described in the nervous system but has been shown to be expressed ovary tissues recently, acting as a paracrine/autocrine regulator required for developments of follicles and oocytes. Although it is generally accepted that chronic stress impairs female reproduction decreases expression BDNF limbic structures central system, which contributes mood disorder. However, not known whether affects oocytes developments, nor ovary....
Objectives The aim of this study was to assess the performance noninvasively prenatal testing (NIPT) for fetal copy number variants (CNVs) in clinical samples, using a whole-genome sequencing method. Method A total 919 archived maternal plasma samples with karyotyping/microarray results, including 33 CNVs and 886 normal from September 1, 2011 May 31, 2013, were enrolled study. randomly rearranged blindly sequenced by low-coverage (about 7M reads) DNA. Fetal detected Copy-number Analysis...
Chronic psychosocial stress negatively affects ovarian function. Ovarian follicular development is regulated by both pituitary-derived gonadotropins and intraovarian regulatory factors. To date, the suppressive effects of chronic on ovary have been observed to be manifested mainly as an inhibition gonadotropin release. It not clear whether there are any other mechanisms involved in this process. Growth differentiation factor 9 (GDF9) important, oocyte-specific paracrine regulator required...
The applications of massively parallel sequencing technology to fetal cell-free DNA (cff-DNA) have brought new insight non-invasive prenatal diagnosis. However, most previous research based on maternal plasma has been restricted aneuploidies. To detect specific parentally inherited mutations, invasive approaches obtain are the current standard in clinic because experimental complexity and resource consumption previously reported approaches.Here, we present a simple effective method for...
The purpose of this study were to develop a methodology isolating fetal cells from maternal blood and use deep sequence demonstrating the promise for complete accurate genetic screening compared other non-invasive prenatal testing.Here in study, we developed double negative selection (DNS) procedure unbiasedly enrich cells. After validated by short tandem repeat (STR), isolated circulating (CFCs) subjected whole genome sequencing analysis.Our DNS protocol significantly increasing purity...