A5073862669- Folate and B Vitamins Research
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Esophageal and GI Pathology
- Cancer-related gene regulation
- RNA modifications and cancer
- Prion Diseases and Protein Misfolding
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Trace Elements in Health
- Sulfur Compounds in Biology
- Ion Transport and Channel Regulation
- Peroxisome Proliferator-Activated Receptors
- Food composition and properties
- Porphyrin Metabolism and Disorders
- Diet, Metabolism, and Disease
- Cancer, Lipids, and Metabolism
- Neurological diseases and metabolism
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Amino Acid Enzymes and Metabolism
- Birth, Development, and Health
- Cerebrovascular and Carotid Artery Diseases
- Iron Metabolism and Disorders
- Cell Adhesion Molecules Research
Pennsylvania State University
2018-2024
University of Lisbon
2014-2024
Centro Hospitalar de Entre o Douro e Vouga E.P.E.
2023
Universidade Nova de Lisboa
2018
Lusíada University of Lisbon
2017
Institute for Biomedical and Pharmaceutical Research
2014
Brigham and Women's Hospital
2011
Universidade de São Paulo
2001-2004
Governador Celso Ramos Hospital
2004
University of Amsterdam
2002
The pathogenic mechanism of homocysteine's effect on cardiovascular risk is poorly understood. Recent studies show that DNA hypomethylation induced by increases in S-adenosylhomocysteine (AdoHcy), an intermediate Hcy metabolism and a potent inhibitor methyltransferases, may be involved homocysteine-related pathology.We measured fasting plasma total (tHcy), AdoHcy, S-adenosylmethionine (AdoMet) methylation leukocytes 17 patients with vascular disease 15 healthy, age- sex-matched...
Methylenetetrahydrofolate reductase (MTHFR) is one of the main regulatory enzymes homocysteine metabolism. Elevated plasma total (tHcy) a major risk for cardiovascular disease. A common 677C-->T mutation in MTHFR gene results decreased enzymic activity, and contributes to increased tHcy, association with low folate. recently described 1298A-->C clearly reduces activity (although lesser extent than 677C-->T) but its effect on tHcy levels not yet clear.To investigate frequency these two...
Increased levels of homocysteine have been established as a risk factor for cardiovascular disease (CVD) by mechanisms still incompletely defined. S-Adenosylhomocysteine (SAH) is the metabolic precursor that accumulates in setting hyperhomocysteinemia and negative regulator most cell methyltransferases. Several observations, summarized current review, support concept SAH, rather than homocysteine, may be culprit CVD has associated with hyperhomocysteinemia. This review examines biosynthesis...
There is an increased risk of colon cancer associated with inflammatory bowel disease (IBD). Dietary fibers (DFs) naturally present in vegetables and whole grains offer numerous beneficial effects on intestinal health. However, the refined DFs health remain unclear. Therefore, we elucidated impact DF inulin colonic inflammation tumorigenesis.Four-week-old wild-type (WT) mice were fed diets containing insoluble cellulose (control) or for 4 weeks. A subgroup was then switched to drinking water...
Atherosclerosis and resulting cardiovascular disease are the leading causes of death in US. Hyperhomocysteinemia (HHcy), or accumulation intermediate amino acid homocysteine, is an independent risk factor for atherosclerosis, but intricate biological processes mediating this effect remain elusive. Several factors regulate homocysteine levels, including activity several enzymes adequate levels their coenzymes, pyridoxal phosphate (vitamin B6), folate B9), methylcobalamin B12). To better...
Hyperhomocysteinemia (HHcy) is a risk factor for atherosclerosis through mechanisms which are still incompletely defined. One possible mechanism involves the hypomethylation of nuclear histone proteins to favor progression atherosclerosis. In previous cell studies, hypomethylating stress decreased specific epigenetic tag (the trimethylation lysine 27 on H3, H3K27me3) promote endothelial dysfunction and activation, i.e., an atherogenic phenotype. Here, we conducted pilot study investigate...
Accumulation of the homocysteine (Hcy) precursor S-adenosylhomocysteine (AdoHcy) may cause cellular hypomethylation in setting hyperhomocysteinemia because cystathionine β-synthase (CBS) deficiency, an inborn error metabolism. To test this hypothesis, DNA and protein arginine methylation status were assessed liver, brain, heart, kidney obtained from a previously described mouse model CBS deficiency. Metabolite levels tissues serum determined by high-performance liquid chromatography or...
Aquaporins (AQPs) are transmembrane channels that facilitate water and glycerol permeation through cell membranes. Recently, the channel AQP1 was suggested to contribute endothelial homeostasis cardiovascular health. Less is known about aquaglyceroporins expression its implication in disease (CVD). We have previously used cultured human cells under a hypomethylating environment study dysfunction activation, phenotype implicated establishment of atherosclerosis CVD. Here, we same model...
<b><i>Background:</i></b> Mesial temporal lobe epilepsy related to hippocampal sclerosis (MTLE-HS) is the most common surgically remediable epileptic syndrome. Ablation of cellular prion protein (PrP<sup>c</sup>) gene (<i>PRNP</i>) enhances neuronal excitability hippocampus in vitro and sensitivity seizure vivo, indicating that PrP<sup>c</sup> might be epilepsy. <b><i>Objective:</i></b> To evaluate genetic contribution <i>PRNP</i> MTLE-HS. <b><i>Methods:</i></b> The coding sequence DNA from...
Background: Imbalance of homocysteine (Hcy) metabolism links with several pathologies; nevertheless, it is poorly characterized in pediatric populations. This study investigated the impact age on plasma concentrations Hcy and relevant biomarkers along correspondent genotype interactions. Methods: A healthy cohort aged 9 (n = 195) 17 128) years old (yo) was studied. Immunoassays GC-MS-SIM-mode quantified levels biomarkers. PCR-RFLP or quantitative-PCR assays assessed common variations related...