Macrophages play a protective role in atherosclerosis, whereas homocysteine (Hcy) is recognized as an independent risk factor for atherosclerosis. Defects macrophage autophagy contribute to the formation of atherosclerotic plaques, and dysregulated energy metabolism closely linked process autophagy. However, regulation by pyruvate dehydrogenase (PDH), key component PDH complex involved metabolic homeostasis, remains poorly understood context atherosclerosis induced Hcy. In our study,...

Accumulating evidence has suggested that homocysteine (Hcy) is an independent risk factor for atherosclerosis (AS). Hcy can promote vascular smooth muscle cell (VSMC) proliferation, which pivotal in the pathogenesis and progression of AS. The aim present study was to investigate epigenetic regulatory mechanism microRNA (miR)‑143‑mediated VSMCs proliferation induced by Hcy. results a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphe‑nyltetrazolium bromide assay revealed VSMC increased 1.39‑fold...

Abstract Atherosclerosis is a serious age‐related disease, which has tremendous impact on health care globally. Macrophage inflammation crucial for the initiation and progression of atherosclerosis, microRNAs (miRNAs) recently have emerged as potent modulators inflammation, while underlying mechanisms its involvement in homocysteine (Hcy)‐mediated macrophage atherosclerosis remain largely unknown. Here, we demonstrated that elevated Hcy inhibits expression miR‐195‐3p, turn enhances IL‐31...

Background: Atherosclerosis and type 2 diabetes mellitus contribute to a large part of cardiovascular events, but the underlying mechanism remains unclear. In this study, we focused on identifying linking genes diagnostic biomarkers effective therapeutic targets associated with these two diseases. Methods: The transcriptomic datasets atherosclerosis were obtained from GEO database. Differentially expressed analysis was performed by R studio software, differential including functional...

It is well established that homocysteine (Hcy) an independent risk factor for atherosclerosis (AS), which characterized by vascular smooth muscle cell (VSMC) proliferation. However, the molecular mechanism underlying AS in VSMCs yet to be elucidated. The aim of this study was investigate potential involvement aberrant DNA methylation platelet‑derived growth (PDGF) gene Hcy‑mediated VSMC proliferation and its mechanism. Cultured human were treated with varying concentrations Hcy....

Atherosclerosis (AS) is a progressive disease of multifactorial origin, which occurs in response to endothelial injury. Increased homocysteine (Hcy) considered major cause dysfunction, oxidative stress and DNA methylation; however, the mechanisms remain be fully elucidated. The aim present study was investigate whether Hcy causes injury cells (ECs) by effect lectin‑like oxidized‑low density lipoprotein receptor‑1 (LOX‑1) methylation through toll‑like receptor 4(TLR4)/nuclear factor...

In the present study, we investigate effect of homocysteine (Hcy) on extracellular-superoxide dismutase (EC-SOD) DNA methylation in aorta mice, and explore underlying mechanism macrophages, trying to identify key targets Hcy-induced EC-SOD changes. ApoE-/- mice are fed different diets for 15 weeks, methyltransferase 1 (DNMT1) expression levels detected by RT-PCR western blot analysis. assessed ntMS-PCR. After overexpression or knockdown following transfection macrophages with pEGFP-N1-DNMT1,...

Atherosclerosis (AS) is a disease induced by multiple factors, including genetic and environmental elements. The aim of the present study to investigate comprehensive effects high cholesterol, methionine diet, apolipoprotein E deficiency (ApoE(-/-)) on pathogenesis AS. ApoE(-/-) mice were fed with cholesterol diet for 15 weeks induce hyperlipidemia hyperhomocysteinemia. methylation levels genomic DNA (gDNA) B1 repetitive elements in aortic tissues measured both methylation-dependent...

Background Hyperhomocysteinemia (HHcy)-mediated dysfunction of endothelial NO system is an important mechanism for atherosclerotic pathogenesis. Dimethylarginine dimethylaminohydrolase (DDAH) the key enzyme degrading asymmetric dimethylarginine (ADMA), which endogenous inhibitor nitric oxide (NO) synthase (eNOS). This study was designed to investigate whether originates from HHcy-mediated aberrant methylation modification in promotor region DDAH2 gene. Methods Human umbilical vein cells...

Hyperhomocysteinemia (HHcy) is a risk factor for cardiovascular disease and has strong correlation with heart failure. However, the effects of HHcy on cardiac tissue remain less well understood. To elucidate role p53-dependent apoptosis in HHcy-induced injury, we fed ApoE(-/-) mice high methionine diet to establish model. Serum Hcy, enzymes, lipids were measured. The protein levels Noxa, DNMT1, caspases-3/9, p53 determined by enzyme-linked immunosorbent assay. Bcl-2 Bax proteins detected...

The aim of the present study was to identify an effective method for detecting early‑phase atherosclerosis (AS), as well provide useful DNA methylation profiles serve biomarkers detection AS. A total 300 individuals (150 AS patients and 150 healthy subjects) were recruited peripheral blood analyses at 12 gene promoter loci using nested methylation‑specific polymerase chain reaction in a test set. Based on set, TIMP metallopeptidase inhibitor 1 (TIMP1), ATP binding cassette subfamily member...

Endoplasmic reticulum (ER) stress is emerging as an important modulator of different pathological process and a mechanism contributing to homocysteine (Hcy)-induced hepar injury. However, the molecular event that Hcy-induced ER in under atherosclerosis background currently unknown. oxidoreductin 1α (ERO1α) plays crucial role maintaining function. In this study, we determined expression ERO1α hyperhomocysteinemia effect hepacytes presence Hcy. HHcy model was established by feeding methionine...

The authors conducted an observational study to explore the association between body fat composition and risk of hyperhomocysteinemia (HHcy) their combined effect on developing cardiovascular disease (CVD). Adults aged 18-74 years from Northwest China Natural Population Cohort: Ningxia Project (CNC-NX) were recruited in this study. Association HHcy was evaluated by logistic regression model. Restricted cubic spline used find nonlinear association. impact interaction CVD using addition model...

Vascular smooth muscle cell (VSMC) proliferation is a primary pathological event in atherosclerosis (AS), and homocysteine (Hcy) an independent risk factor for AS. However, the underlying mechanisms are still lagging. Studies have used combination of methylation promoters multiple genes to diagnose tumors, thus aim current study was investigate role status several VSMCs treated with Hcy. CpG islands were identified platelet‑derived growth (PDGF), p53, phosphatase tensin homologue on...

Abnormal elevation of homocysteine (Hcy) level is closely related to the development and progression chronic kidney disease (CKD), with molecular mechanisms that are not fully elucidated. Given demonstration miR-30a-5p specifically expressed in glomerular podocytes, present study we aimed investigate role potential underlying mechanism podocyte apoptosis induced by Hcy. We found elevated Hcy downregulates expression mice Hcy-treated directly targets 3'-untranslated region (3'-UTR) forkhead...

Endoplasmic reticulum (ER) stress and apoptosis play a critical role in liver injury. oxidoreductase 1α (ERO1α) is an oxidase that exists the luminal side of ER membrane, participating protein folding secretion inhibiting apoptosis, but underlying mechanism on injury induced by homocysteine (Hcy) remains obscure. In this study, hyperhomocysteinemia (HHcy) mice model was established cbs+/- feeding high-methionine diet for 12 weeks; fed with exhibited more severe compared to cbs+/+ mice....

Chronic kidney disease (CKD) is a common and complex in kidneys which has been associated with an increased risk of renal cell carcinoma. Elevated homocysteine (Hcy) levels are known to influence the development progression CKD by regulating podocyte injury apoptosis. To investigate molecular mechanisms triggered podocytes Hcy, we used cbs

Accumulating evidence has shown that the apoptosis of trophoblast cells plays an important role in pathogenesis preeclampsia, and intricate interplay between DNA methylation polycomb group (PcG) protein-mediated gene silencing been highlighted recently. Here, we provide expression nervous system 1 (NSPc1), a BMI1 homologous protein, is significantly elevated during which accelerates cell apoptosis. Since NSPc1 acts predominantly as transcriptional inactivator specifically represses HOXA11...

Abstract Background Atherosclerosis, characterized by abnormal arterial lipid deposition, is an age-dependent inflammatory disease and contributes to elevated morbidity mortality. Senescent foamy macrophages are considered be deleterious at all stages of atherosclerosis, while the underlying mechanisms remain largely unknown. In this study, we aimed explore senescence-related genes in diagnosis for atherosclerotic plaque progression. Methods The atherosclerosis-related datasets were...

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Homocysteine (Hcy) is well recognized to be an independent risk factor for atherosclerosis. Long non-coding RNAs (lncRNAs) are emerging regulators of pathophysiological processes including atherosclerosis, while the underlying mechanisms its involvement in Hcy induced-atherosclerosis remain largely unknown.