Immune responses stimulated by photodynamic therapy (PDT) and photothermal (PTT) are a promising strategy for the treatment of advanced cancer. However, antitumor efficacy PDT or PTT alone is less potent unsustainable against cancer metastasis relapse. In this study, Gd

Abstract Background Mesenchymal stem cell (MSC)-derived exosomes have been recognized as new candidate agents for treating critical-sized bone defects; they promote angiogenesis and may be an alternative to therapy. In this study, we evaluated whether derived from marrow-derived MSCs (BMSCs) preconditioned with a low dose of dimethyloxaloylglycine (DMOG), DMOG-MSC-Exos, exert superior proangiogenic activity in regeneration the underlying mechanisms involved. Methods To investigate effects...

The microneedle (MN) provides a promising strategy for transdermal delivery of exosomes (EXO), in which the therapeutic effects and clinical applications are greatly reduced by fact that EXO can only partially reach injury site passive diffusion. Here, we designed detachable MN array to deliver modified nitric oxide nanomotor (EXO/MBA) Achilles tendinopathy (AT) healing. With releasing EXO/MBA, l-arginine was converted NOS or ROS as driving force. Benefiting from motion ability property MPC...

Distraction osteogenesis (DO) is an effective but lengthy procedure to fully induce bone regeneration in large defects. Accumulating evidence supports the role of exosomes secreted by endothelial progenitor cells (EPC-Exos) stimulating angiogenesis, which closely coupled with osteogenesis. This study aimed investigate whether EPC-Exos promote during DO rats. Exosomes were isolated from supernatants rat marrow EPCs via ultracentrifugation and characterized transmission electron microscopy,...

Abstract Diabetic foot ulcers are a major health‐care burden worldwide. One primary cause of the delayed wound healing in diabetic patients is impaired function hypoxia‐inducible factor‐1α/vascular endothelial growth factor (HIF‐1α/VEGF) axis, which results compromised neovascularization response to hypoxia. In present study, we aimed investigate effect roxadustat, novel HIF prolyl‐4‐hydroxylase inhibitor, on angiogenesis and its therapeutic cutaneous rats. vitro, found that roxadustat could...

Biopolymer-based supramolecular hydrogels cross-linked by host–guest interactions are usually mechanically weak as shown in "inverted vials" instead of freestanding 3D constructs. Herein, we describe a novel macromer (HGM) approach for preparation biopolymer-based hydrogels. Host–guest macromers formed molecular self-assembly between adamantane-functionalized hyaluronic acid (ADxHA) guest polymers and monoacrylated β-cyclodextrins (mono-Ac-βCD) host monomers. Supramolecular readily prepared...

The influence of low-temperature conditioning (LTC) treatment on chilling injury, glycine betaine content, and energy metabolism in loquat fruit at 1 °C storage was investigated. results indicated that LTC significantly reduced injury index, ion leakage, malondialdehyde content fruit. Betaine aldehyde hydrogenase (BADH) activity endogenous (GB) loquats treated with were higher than those control Moreover, induced activities metabolism-associated enzymes, including H+-adenosine...

The morphologies of gold nanoparticles (NPs) affect their tumor accumulation through enhanced permeability and retention effect. However, detailed information mechanisms NPs' characteristics affecting are limited. aim this study is to evaluate the effects shape active targeting ligands theranostic NPs on therapeutic efficacy, elucidate underlying mechanism. Methods:αvβ3 integrin-targeted, cisplatin-loaded radioisotope iodine-125 labeled spherical rod-shaped nano probes (RGD-125IPt-AuNPs...

Stem cell-derived exosomes have exhibited promise for applications in tissue regeneration. However, one major problem stem exosome therapies is identifying appropriate source cells. In the present study, we aimed to compare bone regenerative effect of secreted by marrow mesenchymal cells (BMSCs) derived from type 1 diabetes rats (dBMSC-exos) and BMSCs normal (nBMSC-exos). were isolated with streptozotocin-induced rats. dBMSC-exos nBMSC-exos an ultracentrifugation method identified. The...

Damaged endothelial cells and downregulated osteogenic ability are two key pathogenic mechanisms of glucocorticoid (GC)-induced osteonecrosis the femoral head (ONFH). Recent studies suggested that transplantation CD34+ stem cell-derived exosomes (CD34+-Exos) can treat ischemic diseases by promoting neovascularization miR-26a is an important positive regulator osteogenesis. Moreover, biological effect closely related to their cargo miRNAs. However, it not clear whether increasing abundance in...

High glucose (HG)-mediated bone marrow mesenchymal stem cell (BMSC) dysfunction plays a key role in impaired formation induced by type 1 diabetes mellitus (T1DM). Morroniside is an iridoid glycoside derived from the Chinese herb Cornus officinalis, and it has abundant biological activities associated with metabolism tissue regeneration. However, effects underlying mechanisms of morroniside on HG-induced BMSC remain poorly understood.Alkaline phosphatase (ALP) staining, ALP activity Alizarin...

Abstract Background Osteogenesis is tightly coupled with angiogenesis during bone repair and regeneration. However, the underlying mechanisms linking these processes remain largely undefined. The present study aimed to test hypothesis that epidermal growth factor-like domain-containing protein 6 (EGFL6), an angiogenic factor, also functions in marrow mesenchymal stem cells (BMSCs), playing a key role interaction between osteogenesis angiogenesis. Methods We evaluated how EGFL6 affects...

The emergence of multi-drug resistant (MDR) pathogens is a major public health concern, posing substantial global economic burden. Photothermal therapy (PTT) at mild temperature presents promising alternative to traditional antibiotics due its biological safety and ability circumvent drug resistance. However, the efficacy PTT limited by bacterial thermotolerance. Herein, nanocomposite, BP@Mn-NC, comprising black phosphorus nanosheets manganese-based nanozyme (Mn-NZ) developed, which...

Tendon injures are common orthopedic conditions, but tendon development and the pathogenesis of injures, such as tendinopathy, remain largely unknown have limited clinical therapy. Studies on tenogenic differentiation at molecular level may help in developing novel therapeutic strategies. As regulators, long noncoding RNAs (lncRNAs) been found to widespread biological functions, emerging evidence demonstrates that lncRNAs play important regulatory roles cell tissue regeneration. In this...

Distraction osteogenesis (DO) is one of the most dramatic reconstructive techniques for inducing bone regeneration, but it involves an undesirably long period consolidation. Developing innovative approaches to enhance consolidation a burning need. Human fetal mesenchymal stem cells (hFMSCs) have been shown express more primitive developmental genes than those human adult (hAMSCs), which preferable source cell therapy and tissue regeneration. In present study, we investigated immunogenicity...

Rehmanniae Radix is a traditional herbal medicine in East Asia that has been widely used to treat patients with osteoporosis. However, the effect of catalpol, primary active principle component Radix, on function bone marrow mesenchymal stem cells (BMSCs) and underlying molecular mechanisms associated its activity remain poorly understood. The catalpol proliferation BMSCs was evaluated using Cell Counting Kit-8 assay. Alkaline phosphatase (ALP) staining, ALP Alizarin Red staining were...

Both osteoblasts and preosteoclasts contribute to the coupling of osteogenesis angiogenesis, regulating bone regeneration. Astragaloside IV (AS-IV), a glycoside cycloartane-type triterpene derived from Chinese herb Astragalus membranaceus, exhibits various biological activities, including stimulating angiogenesis attenuating ischemic-hypoxic injury. However, effects underlying mechanisms AS-IV in osteogenesis, osteoclastogenesis, regeneration remain poorly understood. In present study, we...

Cobalt (Co) ions, which can mimic hypoxia to promote angiogenesis, exhibit great potential for bone repair. However, a key point the use of Co ions is that their release profile should be controllable and, more importantly, suitable regeneration process. Here, 2-ethylimidazole (eIm) was introduced into zeolitic imidazolate framework-67 (ZIF-67) slow down Co-ion and fabricate eIm-doped ZIF-67 (eIm/ZIF-67), combined gelatin methacrylate (GelMA) obtain an in situ photo-cross-linking...

Distraction osteogenesis (DO) is a unique technique for promoting bone formation in clinical practice. However the underlying mechanism remains elusive. As epigenetic mediators, microRNAs have been reported to play important roles regulating osteogenesis. In this study, after successfully established DO model of rats, microRNA microarray was performed find molecular targets DO. Total 100 were identified as differently expressed, with miR-503 being one most significantly up-regulated miRNAs...

Hydrogel-based chondrocyte implantation presents a promising tissue engineering strategy for cartilage repair. However, the widely used elastic hydrogels usually restrict cell volume expansion and induce dedifferentiation of encapsulated chondrocytes. To address this limitation, photoannealed granular hydrogel (GH) composed hyaluronic acid, polyethylene glycol, gelatin was formulated regeneration in study. The unannealed GH prepared by Diels-Alder cross-linked microgels could be mixed with...

Casein and α-lactalbumin (α-LA) are the main allergens in cow's milk, which can affect skin, respiratory system, gastrointestinal tract, even cause anaphylactic shock. Developing a rapid sensitive detection method of casein α-LA is still pursued. Herein, surface-enhanced Raman scattering based lateral flow assay (SERS-LFA) for highly milk food was established effectively preventing allergic symptoms. [email protected] nanoparticles ([email NPs) were synthesized as SERS active substrate to...